A Taguchi design approach for the enhancement of a detergent‐biocompatible alkaline thermostable protease production by Streptomyces mutabilis strain TN‐X30

Abstract

The ability of microorganisms to grow at high temperature, alkaline pH, and high salinity makes them an attractive target for enzyme-production with several industrial applications. One strain TN-X30 has been selected as protease producer and identified as Streptomyces mutabilis after a phenotypic and molecular study. Its production of protease was improved using Taguchi L27 design. The strategy was carried out to identify the optimum levels and the interaction of the screened factors. Following this step, maximum protease activity (10,895 U/ml) was achieved after 6-days of incubation. The TN-X30 protease activity had an optimum of pH and temperature of 10 and 65 C, respectively. Thermodynamic parameters at 60 C were enthalpy 14.26 kJ/mol, entropy À220 J/mol/K, and Gibbs free energy 90.53 kJ/mol. TN-X30 protease production displayed a 16-fold increase reaching 175,000 U/ml in a 100-L fermentor. Furthermore, the lyophilization in presence of sorbitol enhanced the stability of the TN-X30 protease which remained active at 75% after 24-months of storage. The lyophilized TN-X30 protease exhibited exceptional stability indexes in presence of some known commercialized detergent components as NEODOL ® 25-7, Dehydol ® LT 7, Na 2 CMC, Galaxy LAS, Galaxy LES 70, Galaxy 110, Galaxy CAPB Plus, and Sulfacid K. The lyophilized enzyme also displayed high stability with respect to both solid and liquid detergents. Finally, TN-X30 protease exhibited remarkable destaining of blood, egg, and chocolate stained cloth pieces. These findings may promote TN-X30 protease for use as bioadditive in detergent formulation, thereby reducing environmental chemical threat.

Characterization of Detergent-Compatible Lipases from Candida albicans and Acremonium sclerotigenum under Solid-State Fermentation

“…The addition of enzymes to laundry detergent can improve washing performance and reduce the amount of detergent needed, thus providing an economic benefit by decreasing the cost and chemical burden caused by detergents . Laundry detergents contain a variety of components, including bleaching agents, builders, and enzymes, which work together to prevent soil deposition onto fabrics, inhibit corrosion, add alkalinity, and maintain buffering capacity . In addition to bleaching agents, builders, and enzymes, laundry detergents also contain surfactants, which reduce the surface tension at the interfaces of oil and water and make it easier to remove dirt.…”

Purification, Biochemical and Kinetic Characterization of a Novel Alkaline sn-1,3-Regioselective Triacylglycerol Lipase from Penicilliumcrustosum Thom Strain P22 Isolated from Moroccan Olive Mill Wastewater

“…Laundry detergents are a mixture of large components, such as bleaching agents, surfactants, enzymes, and builders, preventing the deposition of soil onto fabrics, preventing corrosion, adding alkalinity, and retaining buffering capacity [ 68 ]. Bleaching agents provide the bleaching action through oxidation, whilst the surfactants decrease the surface tension at the interfaces, thus improving the repulsive force and promoting the cleaning process.…”

“…The compatibility of the PCrL with some solid and liquid laundry detergents was determined as detailed previously [ 68 ]. Briefly, each commercial laundry detergent was dissolved in tap water to reach a concentration of 7 mg/mL and treated for 1 h at 65 °C to deactivate endogenous lipases.…”

Valorization of Potato Peels Starch for Efficient β‐Cyclodextrin Production and Purification through an Eco‐Friendly Process

“…[4,5,6] Cyclization remains the most important bio-products including enzymes and CDs. [23,24,25] Within this framework, the Taguchi plan and Response Surface Methodology (RSM) were widely applied in various biotechnological processes.…”

“…[ 22 ] The experimental design approach is also a strategy commonly applied for the production enhancement of bio‐products including enzymes and CDs. [ 23,24,25 ] Within this framework, the Taguchi plan and Response Surface Methodology (RSM) were widely applied in various biotechnological processes.…”