A tandem-repeat galectin-1 from Apostichopus japonicus with broad PAMP recognition pattern and antibacterial activity

Zhang, Chi; Xue, Zhuang; Yu, Zichao; Wang, Hui; Liu, Yu; Li, Huan; Wang, Lingling; Li, Chenghua; Song, Linsheng

doi:10.1016/j.fsi.2020.02.011

Cited by 21 publications

(7 citation statements)

References 45 publications

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“…An interesting example is the presence in many organisms of pattern recognition receptors (known as PRRRs), which are part of the innate immune system, and are deputed to recognition and binding of conserved pathogen associated molecular patterns (known as PAMPs). These tandem repeats often present antibacterial activity when used as peptides [96]. It is likely that in the octopus suckers, tandem repeats play several roles and their eventual release into the medium may be critical for immunity similarly to what has been found for other AMPs present as tandem repeats in host proteins [97][98][99].…”

Section: Discussionmentioning

confidence: 86%

OctoPartenopin: Identification and Preliminary Characterization of a Novel Antimicrobial Peptide from the Suckers of Octopus vulgaris

et al. 2020

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Microorganism resistance to conventional antibiotics represents one of the major global health concerns. This paper focuses on a peptide (OctoPartenopin) extracted from suckers of Octopus vulgaris; bioassay-guided chromatographic fractionation was used to identify this sequence, which holds significant antibacterial activity against Gram-positive and Gram-negative bacteria. OctoPartenopin is encrypted within the calponin sequence and was associated with the high levels of proteolytic activity already reported in octopus arm suckers. We synthesized the parent peptide and four analogues; all peptide were tested for their antibacterial and antibiofilm activities. Preliminary antibiofilm experiments showed that that one of the analogues had the best activity in both inhibition and eradication of biofilm of all three microorganisms tested. The occurrence of OctoPartenopin in arm suckers provided novel speculative information on animal behavior, as concerns maternal care of fertilized eggs. Our results highlight that suckers are a rich source of multifaceted peptides to develop alternative antimicrobial agents and food preservatives.

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Section: Discussionmentioning

confidence: 86%

OctoPartenopin: Identification and Preliminary Characterization of a Novel Antimicrobial Peptide from the Suckers of Octopus vulgaris

et al. 2020

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“…The absorbance of the samples was detected at 405 nm using a microplate reader (Tecan, Infinite M2000 pro, Switzerland). Samples with P (sample)-B (blank)/N (negative)-B (blank) > 2.1 were considered positive (Zhang et al, 2020).…”

Section: Pamp Binding Assay Of Rpdef3mentioning

confidence: 99%

Antimicrobial Defensin and DNA Traps in Manila Clam Ruditapes philippinarum: Implications for Their Roles in Immune Responses

Han

Chen

et al. 2021

Front. Mar. Sci.

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Although defensins have been isolated from a variety of metazoan, their role in cellular immunity has not been answered. In the study, we found that the hemocytes of the Manila clams Ruditapes philippinarum release defensin (designated as Rpdef3) in response to Vibrio parahaemolyticus challenge. The antimicrobial Rpdef3 was proved to be involved in the extracellular traps (ETs) that hemocytes released in response to Vibrio challenge. Scanning electron microscopy observation proved the patterns how ETs eliminate invading bacteria. Furthermore, Rpdef3 involved in ETs had broad-spectrum antimicrobial effect on both Gram-negative bacteria and Gram-positive bacteria. ELISA assay revealed that Rpdef3 could bind lipopolysaccharides and peptidoglycan in a dose-dependent manner. As concerned to the antibacterial mechanisms, Rpdef3 can cause bacterial membrane permeabilization, leading to cell death. As a result, Rpdef3 might contribute to the trap and the elimination of invading Vibrio in clam ETs. Taken together, our study suggest that the formation of ETs is a defense mechanism triggered by bacterial stimulation, coupled with antibacterial defensin.

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“…It is an opportunistic pathogen, which is a major cause of invasive fungal disease, principally in immunocompromised individuals, such as patients with organ transplantation and HIV infection or patients undergoing chemotherapy. The superficial mucosal and dermal infections caused by C. albicans can be disseminated to bloodstream infections with a mortality rate higher than 40% [6].…”

Section: Introductionmentioning

confidence: 99%

The Membranotropic Peptide gH625 to Combat Mixed Candida albicans/Klebsiella pneumoniae Biofilm: Correlation between In Vitro Anti-Biofilm Activity and In Vivo Antimicrobial Protection

Maione

Alteriis

Carraturo

et al. 2021

JoF

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The antibiofilm activity of a gH625 analogue was investigated to determine the in vitro inhibition and eradication of a dual-species biofilm of Candida albicans and Klebsiella pneumoniae, two leading opportunistic pathogens responsible for several resistant infections. The possibility of effectively exploiting this peptide as an alternative anti-biofilm strategy in vivo was assessed by the investigation of its efficacy on the Galleria mellonella larvae model. Results on larvae survival demonstrate a prophylactic efficacy of the peptide towards the infection of each single microorganism but mainly towards the co-infection. The expression of biofilm-related genes in vivo showed a possible synergy in virulence when these two species co-exist in the host, which was effectively prevented by the peptide. These findings provide novel insights into the treatment of medically relevant bacterial–fungal interaction.

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A tandem-repeat galectin-1 from Apostichopus japonicus with broad PAMP recognition pattern and antibacterial activity

Cited by 21 publications

References 45 publications

OctoPartenopin: Identification and Preliminary Characterization of a Novel Antimicrobial Peptide from the Suckers of Octopus vulgaris

OctoPartenopin: Identification and Preliminary Characterization of a Novel Antimicrobial Peptide from the Suckers of Octopus vulgaris

Antimicrobial Defensin and DNA Traps in Manila Clam Ruditapes philippinarum: Implications for Their Roles in Immune Responses

The Membranotropic Peptide gH625 to Combat Mixed Candida albicans/Klebsiella pneumoniae Biofilm: Correlation between In Vitro Anti-Biofilm Activity and In Vivo Antimicrobial Protection

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