A TaqMan PCR assay for detection of DGAT1 K232A polymorphism in cattle

Babii, Anna V.; Andreichenko, Irina N.; Brigida, Artyom Vladimirovich; Kovalchuk, Svetlana N.

doi:10.3934/agrfood.2018.3.306

Cited by 5 publications

(1 citation statement)

References 25 publications

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“…Moreover, the limitation of this technique may be overcome by other sensitive, speci c, with potential for detecting rare alleles and reliable techniques such as the polymerase chain reaction-high resolution melting (PCR-HRM) method. HRM relies on changes in the DNA melting temperature and gradual monitoring of uorescence as the DNA strands break (Babii et al 2018;Herrmann et al 2006). The accuracy of genotyping can be compared with that of other techniques, such as sequencing.…”

Section: Association Of Vdr Gene Variants With Hdp Detected Using Dif...mentioning

confidence: 99%

A plethora of laboratory protocols for vitamin D receptor (VDR) gene variants detection: a systematic review of associations with hypertensive disorders of pregnancy

Ibrahim,

Basri,

Jamil

et al. 2023

Preprint

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Introduction: Hypertensive disorders of pregnancy constitute the major cause of maternal morbidity and mortality. Genetic variation involving VDR gene variants was thought to play a significant role in aetiopathogenesis of HDP. Vitamin D receptor (VDR) gene polymorphisms are thought to be implicated in the development of hypertensive disorders of pregnancy (HDP). However, the association of the variants with HDP is inconsistently reported. The study aims to review the laboratory protocols of VDR variant detection and association with HDP. Methods This study involved one or more of the major VDR gene variants (FokI, BsmI, ApaI, and TaqI) in HDP. The Web of Science, PubMed, Scopus, MEDLINE and CINAHL databases were searched for articles. Preferred Reporting Items for Systematic Review and Meta-analysis (PRISMA) was used. The study was registered in the PROSPERO database (registration number CRD42022362561). Results Our analysis of VDR variant detection protocols revealed that approximately 6 (67%) studies used polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP), of which 3 (33%) reported a significant association with the FokI variant. Two (22%) of the studies used TaqMan PCR and found an association with the FokI variant. Only 1 (11%) study utilized allele-specific PCR (AS-PCR) to genotype the ApaI variant. Based on the analysis of the variants with populations, 4 studies (44%) reported an association with the FokI variant in Asians. Two studies (22%) reported that the BsmI variant is common among Caucasians. Conclusions The detection protocols evaluated were found to be sensitive in detecting some variants in certain populations but not in others, however, the variants were found to be population-specific. Our findings could potentially be useful in stimulating the discovery of distinct biomarkers specific to various populations and could as well prompt the personalised management of hypertension in pregnancy.

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Section: Association Of Vdr Gene Variants With Hdp Detected Using Dif...mentioning

confidence: 99%