“…pullulans can have a complex life-cycle involving hyaline hyphae, yeast-like cells, melanin-pigmented hyphae, swollen cells and chlamydospores (Cooke, 1962;Durrell, 1968;Pechak and Crang, 1977;Gadd, 1980;Gadd and Griffiths, 1980). It has been the subject of a number of studies on cellular differentiation but many of the results presented so far are inconsistent (Brown, 1965;Ramos and Garcia Acha, 1975;Sevilla et al, 1977;Dominguez et al, 1978;Bermejo et al, 1981;Park, 1982a,b).…”