A TCM formula comprising Sophorae Flos and Lonicerae Japonicae Flos alters compositions of immune cells and molecules of the STAT3 pathway in melanoma microenvironment

Liu, Yuxi; Bai, Jing‐Xuan; Li, Ting; Fu, Xiu‐Qiong; Guo, Hui; Zhu, Peili; Chan, Yuen-Cheung; Chou, Ji‐Yao; Yin, Chang-Cheng; Li, Jun‐Kui; Wang, Yaping; Chen, Ying‐Jie; Yu, Zhi‐Ling

doi:10.1016/j.phrs.2019.02.020

Cited by 36 publications

(28 citation statements)

References 34 publications

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“…Human sICAM-1 (Soluble) Enzyme-linked immunosorbent assay (ELISA) Kit was purchased from ExCell Bio (Shanghai, China). Conditioned media from A375 cells after 24 h of LPS (1 µg/mL) or MPLAs (1 µg/mL) stimulation were subjected to ELISA assays following the manufacturer's instructions 12 .…”

Section: Enzyme-linked Immunosorbent Assay (Elisa) Assaymentioning

confidence: 99%

“…Primary antibodies used in this study were Ki-67 (Abcam: ab16667), CD31 (Abcam: ab9498), E-cadherin (CST: #3195), N-cadherin (CST: #13116) and Vimentin (CST: #5741). Positive signals were developed using diaminobenzidine (DAB) substrate (Dako Carpinteria, CA, USA) under the manufacturer recommended conditions and photographed 12 . Experiment and result assessment were conducted blind.…”

Section: Immunohistochemistry Stainingmentioning

confidence: 99%

“…Spleens and tumors from tumor-bearing mice were used to prepare single cell suspensions. Cells incubated with fluorochrome-conjugated antibodies were detected using flow cytometry (12). The anti-CD3 (553063), anti-CD4 (553651), anti-CD8 (551162), anti-NK 1.1 (553164), anti-CD25 (553866), anti-CD86 (553691), anti-CD11c (553802), anti-Gr-1 (561105), anti-CD11b (557397), anti-Foxp3 (563101) and anti-F4/80 (564227) antibodies were bought from BD Biosciences (US).…”

Section: Flow Cytometric Immunophenotypingmentioning

confidence: 99%

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Activation of STAT3 is a key event in TLR4 signaling-mediated melanoma progression

Liu

Wang

et al. 2020

Cell Death Dis

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Malignant melanoma is aggressive and has a high mortality rate. Toll-like receptor 4 (TLR4) has been linked to melanoma growth, angiogenesis and metastasis. However, signal transduction mediated by TLR4 for driving melanoma progression is not fully understood. Signal transducer and activator of transcription 3 (STAT3) has been identified as a major oncogene in melanoma progression. We found: that TLR4 expression positively correlates with activation/phosphorylation of STAT3 in human melanoma samples; that TLR4 ligands activate STAT3 through MYD88 and TRIF in melanoma cells; and that intratumoral activation of TLR4 increases STAT3 activation in the tumor and promotes tumor growth, angiogenesis, epithelial-mesenchymal transition (EMT) and the formation of an immunosuppressive tumor microenvironment in mice. Further, we found that the effects mediated by activating TLR4 are weakened by suppressing STAT3 function with a dominant negative STAT3 variant in melanoma. Collectively, our work identifies STAT3 activation as a key event in TLR4 signaling-mediated melanoma progression, shedding new light on the pathophysiology of melanoma.

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Section: Enzyme-linked Immunosorbent Assay (Elisa) Assaymentioning

confidence: 99%

Section: Immunohistochemistry Stainingmentioning

confidence: 99%

Section: Flow Cytometric Immunophenotypingmentioning

confidence: 99%

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Activation of STAT3 is a key event in TLR4 signaling-mediated melanoma progression

Liu

Wang

et al. 2020

Cell Death Dis

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“…Dioscin (purity >98% as determined by high-performance liquid chromatography) was obtained from Shanghai Yuanye Bio-Technology Co., Ltd. Human A375 melanoma cells, murine B16F10 melanoma cells and murine L929 fibroblasts were obtained from the American Type Culture Collection (ATCC). The B16 STAT3C (overexpressing a constitutively active STAT3 mutant, STAT3C Flag pRc/CMV) and B16 NC (expressing the empty vector, pcDNA 3.0) cell lines were previously established (8). The plasmids were obtained from AddGene Inc. (cat.…”

Section: Methodsmentioning

confidence: 99%

“…Activation of STAT3 promotes cell proliferation, inhibits cell apoptosis and facilitates cell migration in melanoma (7). Overexpression of a dominant-negative STAT3 variant has been demonstrated to result in growth inhibition and regression, and prevents metastasis of melanoma cells in vivo (8). Furthermore, STAT3 knockdown with siRNAs in melanoma cells has been demonstrated to induce apoptosis, and inhibit proliferation and migration (9).…”

Section: Introductionmentioning

confidence: 99%

Inhibiting the Src/STAT3 signaling pathway contributes to the anti‑melanoma mechanisms of dioscin

Liu

Chen

et al. 2020

Oncol Lett

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Late stage melanoma is associated with a high mortality rate. Signal transducer and activator of transcription 3 (STAT3) is currently a target for melanoma treatment as it is constitutively activated with high frequency in melanoma. Dioscin is a natural steroid saponin that is present in several medical herbs. A previous study demonstrated that dioscin inhibits STAT3 signaling in a cerebral ischemia-reperfusion injury rat model. Furthermore, dioscin has been reported to exert anti-melanoma effects in B16 melanoma cells and a B16 allograft mouse model. The present study investigated whether inhibition of STAT3 signaling is involved in the anti-melanoma effects of dioscin. The results of the present study demonstrated that dioscin significantly decreased viability, induced apoptosis and suppressed migration of human A375 melanoma cells and murine B16F10 melanoma cells. Furthermore, dioscin inhibited the phosphorylation of STAT3 and Src (an upstream kinase of STAT3), and downregulated mRNA levels of STAT3-targeted genes, including B-cell lymphoma-2, cyclin D1 and matrix metalloproteinase-2. In addition, overexpression of STAT3 decreased the anti-proliferative effects of dioscin. Overall, the results of the present study indicate that inhibiting the Src/STAT3 signaling pathway contributes to the antimelanoma molecular mechanisms of dioscin. These results provide further pharmacological groundwork for developing dioscin as a novel anti-melanoma agent.

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Leukocyte modulation by natural products from herbal medicines and potential as cancer immunotherapy

Yang

Wang²,

Zhang

2022

Journal of Leukocyte Biology

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Cancer constitutes a kind of life‐threatening disease that is prevalent throughout the world. In light of limitations in conventional chemotherapies or radiotherapies, cancer immunotherapy has emerged as a potent strategy in treating cancer. In cancer immunotherapy, preliminary studies have demonstrated that cancer immune surveillance serves a crucial role in tumor initiation, progression, and metastasis. Herbal medicines and natural products, which serve as alternative medicines, are involved in the modulation of tumor immunosurveillance to enhance antitumor activity. Accordingly, this review aimed to summarize the modulation function of herbal medicines and natural products on tumor immunosurveillance while providing scientific insight into further research on its molecular mechanism and potential clinical applications.

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A TCM formula comprising Sophorae Flos and Lonicerae Japonicae Flos alters compositions of immune cells and molecules of the STAT3 pathway in melanoma microenvironment

Cited by 36 publications

References 34 publications

Activation of STAT3 is a key event in TLR4 signaling-mediated melanoma progression

Activation of STAT3 is a key event in TLR4 signaling-mediated melanoma progression

Inhibiting the Src/STAT3 signaling pathway contributes to the anti‑melanoma mechanisms of dioscin

Leukocyte modulation by natural products from herbal medicines and potential as cancer immunotherapy

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