A Technique for Quantitative Measurement of Endotoxin in Human Plasma

Detection of endotoxins with the Limulus test in burned and unburned mice infected with different species of gramnegative bacteria

“…Endotoxins can be bound to plasma proteins and may be released by the 'pH shift' method described by Reinhold & Fine (1971). In these experiments all plasma was treated with the 'pH shift' method (vide infra).…”

“…Endotoxins were freed from mouse plasma proteins by 'pH shift' method (Reinhold & Fine, 1971). To 1'0 ml.…”

Endotoxin Testing

“…These include addition of perchloric acid (78), chloroform extraction (7,78,79), adsorption of endotoxin on plastic beads (7, SO), alkalinization (81), pH shift (82,83), ammonium sulphate precipitation (7), ether extraction (7), gel filtration (7,82), water dilution (82) and various dilution-heating procedures (7,65,70,(83)(84)(85)(86).…”

MARINE MICROBIOLOGY. Five-Year Progress Report, 1967--1972.

“…During the last year we embarked on a new study involving bacterial for the detection of endotoxins (Levin and Bang, 1964;Rojas-Corona et al, 1969;Reinhold and Fine, 1971;Levin et al, 1970 Since the test is specific for bacterial lipopolysaccharide4 the bacterial biomass can easily be distinguished from the biomass of other organisms .…”