A technique for retrograde intubation in mice

Zhao, Xiaohui; Wu, Nan; Zhou, Jian; Yang, Yongyao; Fang, Yuqiang; Cheng, Wei; Ma, Ruiyan; Tian, Ying; Huang, Lan

doi:10.1038/laban0306-39

Cited by 20 publications

(14 citation statements)

References 4 publications

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“…Repeated measures of airway resistance at different time points has been reported by others 16 . Zhao, et al used a retrograde technique by puncturing the trachea and feeding a guide wire through the hypo-and oropharynx 17 . More recently Singer, et al have performed selective intubation of the left mainstem bronchus in the mouse using an operating microscope and fluoroscopy to document the location of the catheter 18 .…”

Section: Discussionmentioning

confidence: 99%

Endotracheal Intubation in Mice <em>via</em> Direct Laryngoscopy Using an Otoscope

Thomas

Dumouchel

et al. 2014

JoVE

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Mice, both wildtype and transgenic, are the principal mammalian model in biomedical research currently. Intubation and mechanical ventilation are necessary for whole animal experiments that require surgery under deep anesthesia or measurements of lung function. Tracheostomy has been the standard for intubating the airway in these mice to allow mechanical ventilation. Orotracheal intubation has been reported but has not been successfully used in many studies because of the substantial technical difficulty or a requirement for highly specialized and expensive equipment. Here we report a technique of direct laryngoscopy using an otoscope fitted with a 2.0 mm speculum and using a 20 G intravenous catheter as an endotracheal tube. We have used this technique extensively and reliably to intubate and conduct accurate assessments of lung function in mice. This technique has proven safe, with essentially no animal loss in experienced hands. Moreover, this technique can be used for repeated studies of mice in chronic models.

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Section: Discussionmentioning

confidence: 99%

Endotracheal Intubation in Mice <em>via</em> Direct Laryngoscopy Using an Otoscope

Thomas

Dumouchel

et al. 2014

JoVE

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“…e intubation method was adapted from the operation guidelines by Hallowell EMC and previous literature [25][26][27]. Mice were anesthetized intraperitoneally (i.p.)…”

Section: Tracheal Infection With Influenza a Virus (H1n1)mentioning

confidence: 99%

Secretory IgA in Mucosa of Pharynx and Larynx Plays an Important Role against Influenza A Virus Infection in Kidney Yang Deficiency Syndrome Model

Zhao

Yuan

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Objective. Influenza virus poses a major threat to human health and has serious morbidity and mortality which commonly occurs in high-risk populations. Pharynx and larynx of the upper respiratory tract mucosa is the first defense line against influenza virus infection. However, the ability of the pharynx and larynx organ to eliminate the influenza pathogen is still not clear under different host conditions. Methods. In this study, a mouse model of kidney yang deficiency syndrome (KYDS) was used to mimic high-risk peoples. Two different methods of influenza A (H1N1) virus infection by nasal dropping or tracheal intubation were applied to these mice, which were divided into four groups: normal intubation (NI) group, normal nasal dropping (ND) group, model intubation (MI) group, and model nasal dropping (MD) group. e normal control (NC) group was used as a negative control. Body weight, rectal temperature, and survival rate were observed every day. Histopathologic changes, visceral index, gene expressions of H1N1, cytokine expressions, secretory IgA (SIgA) antibodies of tracheal lavage fluids in the upper respiratory tract, and bronchoalveolar lavage fluids were analyzed by ELISA. Results. e MD group had an earlier serious morbidity and mortality than the others. MI and NI groups became severe only in the 6 th to 7 th day after infection. e index of the lung increased significantly in NI, MI, and MD groups. Conversely, indices of the thymus and spleen increased significantly in NC and ND groups. H&E staining showed severe tissue lesions in MD, MI, and NI groups. H1N1 gene expressions were higher in the MD group compared with the MI group on the 3 rd day; however, the MD group decreased significantly on the 7 th day. IL-6 levels increased remarkably, and SIgA expressions decreased significantly in the MD group compared with the NC group. Conclusions. SIgA secretions are influenced directly by different conditions of the host in the pharynx and larynx in the upper respiratory tract mucosa. In the KYDS virus disease mode, SIgA expressions could be inhibited severely, which leads to serious morbidity and mortality after influenza A virus infection. e SIgA expressions of the pharynx and larynx would be an important target in highrisk populations against the influenza A virus for vaccine or antiviral drugs research.

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“…Many researchers have postulated various methods to carry out intubation of the mouse lung in order to assess both pulmonary function and BAL in the individual mice with longitudinal studies [19][20][21][22][23]. Such techniques appear to be largely successful but they are quite expensive and require intense training for the operator.…”

Section: Clinical Utility Of Balf Collectionmentioning

confidence: 99%

Aspiration techniques for bronchoalveolar lavage in translational respiratory research: Paving the way to develop novel therapeutic moieties

Dua

Shukla

Hansbro³

2017

J Biol Methods

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Bronchoalveolar lavage (BAL) is a simple, yet informative tool in understanding the immunopathology of various lung diseases via quantifying various inflammatory cells, cytokines and growth factors. At present, this traditional method is often blended with several robust and sophisticated molecular and biological techniques sustaining the significance and longevity of this technique. Crucially, the existence of slightly distinct approaches and variables employed at different laboratories around the globe in performing BAL aspiration indeed demands an utmost need to optimize and develop an effective, cost-effective and a reproducible technique. This mini review will be of importance to the biological translational scientist, particularly respiratory researchers in understanding the fundamentals and approaches to apply and consider with BAL aspiration techniques. This will ensure generating a meaningful and clinically relevant data which in turn accelerate the development of new and effective therapeutic moieties for major respiratory conditions.

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A technique for retrograde intubation in mice

Cited by 20 publications

References 4 publications

Endotracheal Intubation in Mice <em>via</em> Direct Laryngoscopy Using an Otoscope

Endotracheal Intubation in Mice <em>via</em> Direct Laryngoscopy Using an Otoscope

Secretory IgA in Mucosa of Pharynx and Larynx Plays an Important Role against Influenza A Virus Infection in Kidney Yang Deficiency Syndrome Model

Aspiration techniques for bronchoalveolar lavage in translational respiratory research: Paving the way to develop novel therapeutic moieties

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