A Temperature‐Responsive Boronate Core Cross‐Linked Star (CCS) Polymer for Fast and Highly Efficient Enrichment of Glycoproteins

Abstract

Fast and highly efficient enrichment and separation of glycoproteins is essential in many biological applications, but the lack of materials with high capture capacity, fast, and efficient enrichment/separation makes it a challenge. Here, a temperature‐responsive core cross‐linked star (CCS) polymer with boronate affinity is reported for fast and efficient enriching and separating of glycoproteins from biological samples. The temperature‐responsive CCS polymers containing boronic acid in its polymeric arms and poly(N‐isopropyl acrylamide) in its cross‐linked core are prepared using reversible addition‐fragmentation chain transfer polymerization via an “arm‐first” methodology. The soluble boronate polymeric arms of the CCS polymers provide a homogeneous reaction system and facilitate interactions between boronic acid and glycoproteins, which leads to a fast binding/desorption speed and high capture capacity. Maximum binding capacity of the prepared CCS polymer for horseradish peroxidase is determined to be 210 mg g−1, which can be achieved within 20 min. More interestingly, the temperature‐responsive CCS polymers exhibit rapid reversible thermal‐induced volume phase transition by increasing the temperature from 15 to 30 °C, resulting in a facile and convenient sample collection and recovery for the target glycoproteins. Finally, the temperature‐responsive CCS polymer is successfully applied to enrichment of low abundant glycoproteins.

Hydrophilic carrageenan functionalized magnetic carbon‐based framework linked by silane coupling agent for the enrichment of N‐glycopeptides from human saliva

“…The enrichment recovery of Carr@PEI@mSiO 2 @G@ Fe 3 O 4 on the glycopeptides was evaluated via the isotope labeling method. In brief, IgG was dealt with trypsin in H 2 16 O and H 2 18 O to get 16 O-and 18 O-labeled IgG digests.…”

“…At present, the main enrichment methods that have been developed and used include lectin affinity method [14], boronic acid method [15,16], hydrazine chemical method [17], hydrophilic interaction method, and so on [18]. Among them, it is worth mentioning that because the hydrophilic interaction method has significant advantages such as low bias to different glycopeptides, better compatibility, high throughput, simplicity, and efficiency, it is widely used in the enrichment and separation of glycopeptides [19][20][21][22][23][24].…”

Preparation of Boronic Acid‐Functionalized Silica Nanocomposites for Selective Enrichment of Glycoproteins

“…modified the boronic acid groups simply on the surface of magnetic nanospheres based on the click chemistry and applied them to separate and enrich glycoproteins, which had outstanding performance and promising potential in real biological samples. Chen et al . prepared temperature‐responsive core cross‐linked star (CCS) polymer to apply for enrichment of low abundant glycoproteins, using reversible addition‐fragmentation chain transfer polymerization via an ‘arm‐first’ method.…”