A testis-derived macroporous 3D scaffold as a platform for the generation of mouse testicular organoids

Topraggaleh, Tohid Rezaei; Valojerdi, Mojtaba Rezazadeh; Montazeri, Leila; Baharvand, Hossein

doi:10.1039/c8bm01001c

Cited by 67 publications

(37 citation statements)

References 49 publications

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“…We observed that the percentage of swelling slowly increased with the increase of the incubation time. Two groups of SDS 0.5, 30 and ST 0.5 for 30 min showed the maximum absorption of water after 72 h, about 60%, which is consistent with the similar investigations elsewhere [47]. Pore size is critical Fig.…”

Section: Discussionsupporting

confidence: 91%

“…In our study, the percentage of scaffolds degradation slowly increased with increasing the incubation time, the weight of the scaffold remained intact, nearly 80 percent, following 30 days incubation. SDS 0.5, 30, and ST 0.5 for 30 min showed the maximum degradation rate because of having the highest hydrophilicity [47]. The swelling behavior of scaffolds facilitates cell migration, proliferation, and oxygen absorption and waste disposal [48].…”

Section: Discussionmentioning

confidence: 98%

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Optimization of decellularized human placental macroporous scaffolds for spermatogonial stem cells homing

Asgari

Najafi

et al. 2021

J Mater Sci: Mater Med

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Decellularized scaffolds have been found to be excellent platforms for tissue engineering applications. The attempts are still being made to optimize a decellularization protocol with successful removal of the cells with minimal damages to extracellular matrix components. We examined twelve decellularization procedures using different concentrations of Sodium dodecyl sulfate and Triton X-100 (alone or in combination), and incubation time points of 15 or 30 min. Then, the potential of the decellularized scaffold as a three-dimensional substrate for colony formation capacity of mouse spermatogonial stem cells was determined. The morphological, degradation, biocompatibility, and swelling properties of the samples were fully characterized. The 0.5%/30 SDS/Triton showed optimal decellularization with minimal negative effects on ECM (P ≤ 0.05). The swelling ratios increased with the increase of SDS and Triton concentration and incubation time. Only 0.5%/15 and 30 SDS showed a significant decrease in the SSCs viability compared with other groups (P < 0.05). The SSCs colony formation was clearly observed under SEM and H&E stained slides. The cells infiltrated into the subcutaneously implanted scaffold at days 7 and 30 post-implantation with no sign of graft rejection. Our data suggest the %0.5/30 SDS/Triton as an excellent platform for tissue engineering and reproductive biology applications.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 98%

Optimization of decellularized human placental macroporous scaffolds for spermatogonial stem cells homing

Asgari

Najafi

et al. 2021

J Mater Sci: Mater Med

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“…These structures showed the development of a functional testicular blood barrier between the Sertoli cells without differentiation of the spermatogonial cells [105]. An additional study showed the development of mouse spermatogonial cells to meiotic and post meiotic cells in organoids when cultured in fabricated testis-derived scaffolds [106].…”

Section: Testicular Organoidsmentioning

confidence: 98%

Approaches and Technologies in Male Fertility Preservation

Huleihel

Lunenfeld

2020

IJMS

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Male fertility preservation is required when treatment with an aggressive chemo-/-radiotherapy, which may lead to irreversible sterility. Due to new and efficient protocols of cancer treatments, surviving rates are more than 80%. Thus, these patients are looking forward to family life and fathering their own biological children after treatments. Whereas adult men can cryopreserve their sperm for future use in assistance reproductive technologies (ART), this is not an option in prepubertal boys who cannot produce sperm at this age. In this review, we summarize the different technologies for male fertility preservation with emphasize on prepubertal, which have already been examined and/or demonstrated in vivo and/or in vitro using animal models and, in some cases, using human tissues. We discuss the limitation of these technologies for use in human fertility preservation. This update review can assist physicians and patients who are scheduled for aggressive chemo-/radiotherapy, specifically prepubertal males and their parents who need to know about the risks of the treatment on their future fertility and the possible present option of fertility preservation.

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“…7 Paclitaxel and docetaxel are two classical microtubule inhibitors that exert their activity by promoting tubulin polymerization and stabilization of microtubules, which results in G2-M phase arrest and mitotic cell death. 8,9 They have been widely adopted in treating breast cancer and pancreatic cancer patients. Despite being quite effective, severe hypersensitivity and other potentially dramatic side effects can be caused by paclitaxel and docetaxel.…”

Section: Introductionmentioning

confidence: 99%

<p>The Anti-Tumor Effect of Nab-Paclitaxel Proven by Patient-Derived Organoids</p>

Xiao

Chen

Wei

et al. 2020

OTT

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Background: Nab-paclitaxel has been widely used in treating breast cancer and pancreatic patients for its low toxicity and high efficiency. However, its role in gastric cancer (GC) remains ambiguous. The aim of our study was to test the anti-tumor activity of nab-paclitaxel using GC patient-derived organoids. Methods: By using the organoid culture system, we describe the establishment of human gastric cancer organoid lines from surgical samples of three patients with gastric cancer. The consistency of these organoids with original cancer tissues was evaluated by histopathological examination. The characteristics of the cancer organoids were tested using immunofluorescence (IF) staining. Using organoids, the anti-tumor efficiencies of nab-paclitaxel, 5-Fu and epirubicin were compared by CCK8 assay and Annexin V-FITC/PI staining. Results: Three organoids were successfully established and passaged. The morphology of the established GC organoids was consistent with original cancer tissues. The IC50 of nabpaclitaxel was 3.68 μmol/L in hGCO1, 2.41 μmol/L in hGCO2 and 2.91 μmol/L in hGCO3, which was significantly lower than those of 5-FU (72.99 μmol/L in hGCO1, 28.32 μmol/L in hGCO2 and 2.91 μmol/L in hGCO3) and epirubicin (25.85μmol/L in hGCO1, 15.15 μmol/L in hGCO2 and 7.60 μmol/L in hGCO3). When each organoid lines were treated with nabpaclitaxel for increasing period of time, the percentage of the apoptotic cells in each organoid increased accordingly. Conclusion: Nab-paclitaxel showed strong anti-tumor activity and had the potential to become front-line drug for treating GC patients. Gastric cancer organoid may be a good tool to predict in vivo response to drugs.

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A testis-derived macroporous 3D scaffold as a platform for the generation of mouse testicular organoids

Abstract: Extracellular matrix-derived scaffolds provide an efficient platform for the generation of organ-like structures.

Cited by 67 publications

References 49 publications

Optimization of decellularized human placental macroporous scaffolds for spermatogonial stem cells homing

Optimization of decellularized human placental macroporous scaffolds for spermatogonial stem cells homing

Approaches and Technologies in Male Fertility Preservation

<p>The Anti-Tumor Effect of Nab-Paclitaxel Proven by Patient-Derived Organoids</p>

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