A Tetraploid Induction in Hypericum patulum Thunberg by Colchicine Soaking Treatment

Abstract

-This study aimed to get the basic data on the breeding of good varieties in Hypericum patulum Thunberg. The optimum materials, concentration and soaking time were examined to identify the effective approach to induce tetraploid plant by colchicine treatment to cultivate the varieties. For the seed germination rate of seed by colchicine treatment, the higher colchicine concentration was and the longer soaking time was, the more the germination rate decreased. While individuals were germinated in 16 test groups except control group (no treatment group), all the plants were diploid and no tetraploid was induced. For the plant regeneration rate by colchicine treatment on the explant of Hypericum patulum Thunberg that was under in vitro culture, the higher the colchicine concentration increased, the ress the regeneration rate. While total 147 individuals were regenerated in all treatment, when the explant was soaking treatment in more than 0.05% for over 6 hours, tetraploid could be obtained. In the soaking treatment of 0.05% for over 6 hours, tetraploid could be obtained. In particular, for the soaking treatment in 0.05% for 12 hours, 8 tetraploids were induced, which was about 47.1% of the number of plant regenerated. In accordance with the observation on doubling of DNA contents in leaf in order to identify polyploidy, the peak DNA content of G1 phase was 94.5 for diploid and 192.5 for tetraploid. It confirmed doubling of DNA content. Furthermore, the number of chloroplasts per guard cell depending on polyploid was around 10 in diploid and 17 to 19 in tetraploid, which were around 1.7 to 1.9 times as much as diploid.

Effect of Colchicine on the Induction of Prunella vulgaris for. albiflora Nakai

“…However, in the case of plots treated for 6 and 12 hours at the concentration of 0.05%, and the inducted 18, 19 tetraploid plants showed the highest results respectively. These results were very similar to the results that the tetraploid plants induction using in vitro culture of Hypericum perforatum, the highest induction rate was shown when immersed with colchicine 0.05% for 12 hours (Kwon et al, 2013).…”

Effect of Colchicine on Chromosome Doubling in Codonopsis lanceolata

“…Polyploid organisms have multiplied sets of the same genomes, and in comparison to diploids, there is no qualitative change in genetic information, but, because of the quantitative increase of genetic materials, formative and physiological characters of the plant change (Kwon et al, 2013;Lapin, 1975). Artificial induction of tetraploid plants is generally achieved based on colchicine concentration, treatment time, and treatment method (Kim et al, 2003;Suh, 2005).…”