A Thermosetting Oil for Droplet‐Based Real‐Time Monitoring of Digital PCR and Cell Culture

Wu, Wenrui; Zhou, Shufang; Hu, Jiumei; Wang, Guoping; Ding, Xiong; Gou, Tong; Sun, Jingjing; Zhang, Tao; Mu, Ying

doi:10.1002/adfm.201803559

Cited by 20 publications

(13 citation statements)

References 25 publications

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“…Through distributing targets into isolated microreactions with nanoliter or picoliter volume, dPCR can realize single nucleic acid detection after amplification but suffers from long reaction time. [ 147,148 ] Considering the short reaction time of photonic PCR, some groups have done preliminary work in exploring photonic digital NAAR to achieve ultrafast and ultrasensitive infective pathogens detection. [ 140,149 ] Photonic digital NAAR with the merits of high sensitivity and short reaction time show bright prospective in POC diagnosis and could be a powerful technology in response to the next virus outbreak.…”

Section: Other Methods With Opticsmentioning

confidence: 99%

On‐Chip Optical Detection of Viruses: A Review

Shi

Liu

et al. 2021

Advanced Photonics Research

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The current outbreak of the coronavirus disease‐19 (COVID‐19) pandemic worldwide has caused millions of fatalities and imposed a severe impact on our daily lives. Thus, the global healthcare system urgently calls for rapid, affordable, and reliable detection toolkits. Although the gold‐standard nucleic acid amplification tests have been widely accepted and utilized, they are time‐consuming and labor‐intensive, which exceedingly hinder the mass detection in low‐income populations, especially in developing countries. Recently, due to the blooming development of photonics, various optical chips have been developed to detect single viruses with the advantages of fast, label‐free, affordable, and point of care deployment. Herein, optical approaches especially in three perspectives, e.g., flow‐free optical methods, optofluidics, and surface‐modification‐assisted approaches, are summarized. The future development of on‐chip optical‐detection methods in the wave of emerging new ideas in nanophotonics is also briefly discussed.

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Section: Other Methods With Opticsmentioning

confidence: 99%

On‐Chip Optical Detection of Viruses: A Review

Shi

Liu

et al. 2021

Advanced Photonics Research

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“…The droplet dPCR images with uneven illumination are acquired from the previous work of Wu et al in Zhejiang University [23]. And the experimental dataset of Chip-based dPCR with complex irregular noise is derived from the work of our lab.…”

Section: A Dataset Resource and Preparationmentioning

confidence: 99%

A New Few-Shot Learning Method of Digital PCR Image Detection

Zhang

Chen

et al. 2021

IEEE Access

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With the global pandemic of infectious diseases, the demand for accurate nucleic acid detection is daily increasing. The traditional threshold-based algorithms are adopted as the mainstream for processing the images of digital polymerase chain reaction (dPCR) now, but they are facing huge challenges on complex problems such as irregular noise, uneven illumination, and the lack of data. So, this paper proposed a novel few-shot learning method based on our improved YOLOv3 model with fast processing speed and high accuracy to deal with complicated situations. Besides, to reduce the requirement of the large training dataset and annotation time of deep neural networks, we proposed the Random Background Transfer Method (RBTM) and Source Traceability Annotation Method (STAM) as the data augmentation and annotation method separately, which exploit the prior knowledge of the data and successfully realized the few-shot learning. Bases on the domain knowledge of dPCR images, our method could effectively augment images and reduce the labeling time by 70% while retaining the visually prominent features and improves the detection accuracy from 63.96% of the traditional threshold-based algorithm to as high as 98.98%. With the optimal processing speed and accuracy, our method is the state-of-art strategy for the detection of dPCR images now.

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“…In recent years, droplet-based microfluidic PCR is being widely used in the amplification of nucleic acids 31 . In comparison to typical microfluidic PCR, droplet-based microfluidic PCR benefits from low reagent consumption 32 , low waste production 33 , negligible sample contamination 34 , insignificant surface adsorption 35 , high-speed reactions 36 , and independent monitoring of each droplet 37 , 38 .…”

Section: Introductionmentioning

confidence: 99%

A plasmonic gold nanofilm-based microfluidic chip for rapid and inexpensive droplet-based photonic PCR

Jalili

Bagheri

Shamloo

et al. 2021

Sci Rep

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Polymerase chain reaction (PCR) is a powerful tool for nucleic acid amplification and quantification. However, long thermocycling time is a major limitation of the commercial PCR devices in the point-of-care (POC). Herein, we have developed a rapid droplet-based photonic PCR (dpPCR) system, including a gold (Au) nanofilm-based microfluidic chip and a plasmonic photothermal cycler. The chip is fabricated by adding mineral oil to uncured polydimethylsiloxane (PDMS) to suppress droplet evaporation in PDMS microfluidic chips during PCR thermocycling. A PDMS to gold bonding technique using a double-sided adhesive tape is applied to enhance the bonding strength between the oil-added PDMS and the gold nanofilm. Moreover, the gold nanofilm excited by two light-emitting diodes (LEDs) from the top and bottom sides of the chip provides fast heating of the PCR sample to 230 °C within 100 s. Such a design enables 30 thermal cycles from 60 to 95 °C within 13 min with the average heating and cooling rates of 7.37 ± 0.27 °C/s and 1.91 ± 0.03 °C/s, respectively. The experimental results demonstrate successful PCR amplification of the alcohol oxidase (AOX) gene using the rapid plasmonic photothermal cycler and exhibit the great performance of the microfluidic chip for droplet-based PCR.

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A Thermosetting Oil for Droplet‐Based Real‐Time Monitoring of Digital PCR and Cell Culture

Cited by 20 publications

References 25 publications

On‐Chip Optical Detection of Viruses: A Review

On‐Chip Optical Detection of Viruses: A Review

A New Few-Shot Learning Method of Digital PCR Image Detection

A plasmonic gold nanofilm-based microfluidic chip for rapid and inexpensive droplet-based photonic PCR

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