A Thermostable Type I Collagen from Swim Bladder of Silver Carp (Hypophthalmichthys molitrix)

Abstract

As a major component of the extracellular matrix, collagen has been used as a biomaterial for many purposes including tissue engineering. Commercial collagen derived from mammals is associated with a risk of prion diseases and religious restrictions, while fish-derived collagen can avoid such issues. In addition, fish-derived collagen is widely available and low-cost; however, it often suffers from poor thermal stability, which limits its biomedical application. In this study, collagen with a high thermal stability was successfully extracted from the swim bladder of silver carp (Hypophthalmichthys molitrix) (SCC). The results demonstrated that it was a type I collagen with high purity and well-preserved triple-helix structure. Amino acid composition assay showed that the amounts of threonine, methionine, isoleucine and phenylalanine in the collagen of swim bladder of silver carp were higher than those of bovine pericardium. After adding Tris solution, swim-bladder-derived collagen could form fine and dense collagen fibers. In particular, SCC exhibited a higher thermal denaturation temperature (40.08 °C) compared with collagens from the swim bladder of grass carp (Ctenopharyngodon idellus) (GCC, 34.40 °C), bovine pericardium (BPC, 34.47 °C) and mouse tail (MTC, 37.11 °C). Furthermore, SCC also showed DPPH radical scavenging ability and reducing power. These results indicate that SCC presents a promising alternative source of mammalian collagen for pharmaceutical and biomedical applications.

Swim bladder-derived biomaterials: structures, compositions, properties, modifications, and biomedical applications

“…The swim bladder is composed of high-value substances such as collagen, elastin, and polysaccharides [ 47 , 60 ]. Collagen and elastin make up collagen and elastic fibers, respectively.…”

“…Collagen in the swim bladder is type I collagen [ 61 ], which consists of two α1 and one α2 chains, all of which are left-handed polypeptide chains, arranged in a right-handed triple helix structure. Each chain contains approximately 1,014 amino acids, among which glycine is the most abundant (30%), followed by proline, hydroxyproline, threonine, methionine, isoleucine, and phenylalanine [ 60 , 62 ]. The composition of swim bladder collagen (SBC) is similar to that of traditional bovine pericardium collagen [ 12 ], but the amino acid content in different fish species differs from that in mammals.…”

“…The composition of swim bladder collagen (SBC) is similar to that of traditional bovine pericardium collagen [ 12 ], but the amino acid content in different fish species differs from that in mammals. For example, the amounts of threonine, methionine, isoleucine, and phenylalanine in Silver carp SBC are higher than those in bovine pericardium, but the amounts of other amino acids are similar or lower than those in bovine pericardium [ 60 , 63 ].…”