A tissue-specific, injectable acellular gel for the treatment of chronic vocal fold scarring

Xu, Chet C.; Mau, Ted

doi:10.1016/j.actbio.2019.08.025

Cited by 16 publications

(12 citation statements)

References 82 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These resulted suggested that the digested form retained some of the bioactivity or components recognizable by the kidney stem cells even after pepsin digestion . Furthermore, Xu et al identified some of the previously discussed VFLP-ECM proteins as fragments larger than 7 kDa in their derived bovine VF-ECMh with an aligned discussion regarding their potential effect on the TGF-β1 pathway . In summary, the proteomic comparison between the intact VFLP-ECM and UBM-ECM confirmed the distinctive composition of the VFLP-ECM when compared to a commercially available scaffold and highlights potential compositional differences that could reduce myofibroblast activation in vivo.…”

Section: Discussionmentioning

confidence: 78%

Porcine Vocal Fold Lamina Propria-Derived Biomaterials Modulate TGF-β1-Mediated Fibroblast Activation in Vitro

Mora-Navarro

Badileanu

Martins

et al. 2020

ACS Biomater. Sci. Eng.

View full text Add to dashboard Cite

The vocal fold lamina propria (VFLP), one of the outermost layers of the vocal fold (VF), is composed of tissue-specific extracellular matrix (ECM) proteins and is highly susceptible to injury. Various biomaterials have been clinically tested to treat voice disorders (e.g., hydrogels, fat, and hyaluronic acid), but satisfactory recovery of the VF functionality remains elusive. Fibrosis or scar formation in the VF is a major challenge, and the development and refinement of novel therapeutics that promote the healing and normal function of the VF are needed. Injectable hydrogels derived from native tissues have been previously reported with major advantages over synthetic hydrogels, including constructive tissue remodeling and reduced scar tissue formation. This study aims to characterize the composition of a decellularized porcine VFLP-ECM scaffold and the cytocompatibility and potential antifibrotic properties of a hydrogel derived from VFLP-ECM. In addition, we isolated potential matrix-bound vesicles (MBVs) and macromolecules from the VFLP-ECM that also downregulated smooth muscle actin ACTA2 under transforming growth factor-beta 1 (TGF-β1) stimulation. The results provide evidence of the unique protein composition of the VFLP-ECM and the potential link between the components of the VFLP-ECM and the inhibition of TGF-β1 signaling observed in vitro when transformed into injectable forms.

show abstract

Section: Discussionmentioning

confidence: 78%

Porcine Vocal Fold Lamina Propria-Derived Biomaterials Modulate TGF-β1-Mediated Fibroblast Activation in Vitro

Mora-Navarro

Badileanu

Martins

et al. 2020

ACS Biomater. Sci. Eng.

View full text Add to dashboard Cite

show abstract

“…However, natural materials do have the potential to induce inflammatory activity via immunogenic impurities or antigens. [126,127] For instance, most dECM-based vocal fold biomaterials are animal sourced (e.g., bovine, [128,129] porcine, [130] etc. ), which may increase the potential for xenogeneic immune responses following in vivo implantation.…”

Section: Association Between Chemical Properties and Immunological Outcomesmentioning

confidence: 99%

“…With technical advances in biomaterial fabrication, current trends have been steered toward biomaterial designs that recapitulate the native microstructure of the organ. That said, a balance between design complexity and manufacturing capabilities is (2010) [128] Bovine [129] Porcine bladder ECM 2016) [208] a)…”

Section: What Is Next For Vocal Fold Regenerative Biomaterials?mentioning

confidence: 99%

Progress in Vocal Fold Regenerative Biomaterials: An Immunological Perspective

Coburn

et al. 2021

Advanced NanoBiomed Research

View full text Add to dashboard Cite

The vocal folds are functional organs located in the larynx that play a critical role in our daily breathing, speech, and swallowing functions. A key structural feature of the vocal folds is their delicate mucosa, which consists of a thin, layered epithelium and underlying extracellular matrix (ECM)-rich lamina propria. [1,2] Irreversible changes to the vocal fold mucosa, such as scarring, atrophy, and sulcus vocalis, require regenerative technologies to stimulate a controlled regrowth of the tissue. An increasing number of biomaterial systems have been proposed with the concurrent capability of refilling and regenerating severely damaged or absent vocal fold mucosae in situ (see review on public health significance in the study by Green et al. [3] ).Notably, as the vocal folds are located at the narrowest point of the airway, adverse hostÀbiomaterial responses can impair upper airway patency and create life-threatening complications. For instance, 2À5% of patients were reported to have postoperative inflammatory reactions including edema, dysphonia, and dyspnea to injectable biomaterials derived from hyaluronic acid (HA). [4][5][6] Although the incidence is rare, severe airway reactions could lead to an emergency admission for intensive care and require intubation procedures for breathing support. [5,7] Inevitably, this immunemediated rejection response is detrimental to the longevity, performance, and integration of the biomaterial in the vocal fold mucosa.Upon biomaterial introduction into a host, the immune system quickly responds to the iatrogenic trauma and foreign

show abstract

“…Therefore, to successfully treat VF scarring, the normal structure of the ECM and the pliability of the SLP need to be restored. 8,9 In the last 10 years, several cytokines have been thoroughly investigated. Among these cytokines, the available evidence regarding prostaglandin E2 10 (PGE2), granulocyte-macrophage colony-stimulating factor 11 (GM-CSF), interleukin-10 12 (IL-10), transforming growth factor-β3 13 (TGF-β3), basic fibroblast growth factor (bFGF), 14 and hepatocyte growth factor (HGF) 15 suggests that cytokine treatment improves subjective parameters, histological examination results, and cell fibrosis-related behavior and shows potential for restoring VF scars.…”

Section: Introductionmentioning

confidence: 99%

“…The main histological characteristics of VF scarring are disorganized composition of the extracellular matrix (ECM) and reduced pliability of the superficial layer of the lamina propria (SLP) within the VF in a delayed wound‐healing process; 5–7 these characteristics have been confirmed in both animal models and human pathological tissue. Therefore, to successfully treat VF scarring, the normal structure of the ECM and the pliability of the SLP need to be restored 8,9 . In the last 10 years, several cytokines have been thoroughly investigated.…”

Section: Introductionmentioning

confidence: 99%

The Role of Cytokines in Modulating Vocal Fold Fibrosis: A Contemporary Review

Fan

2020

The Laryngoscope

View full text Add to dashboard Cite

Objectives Vocal fold (VF) scarring and laryngeal stenosis are a significant clinical challenge. Excessive scar formation causes low voice quality or even life‐threatening obstructions. Cytokines are thought to modulate multiple steps of the establishment of VF fibrosis, but there is no systematic report regarding their role in modulating VF fibrosis. This review aims to investigate the role of cytokines in modulating vocal fold fibrosis. Study Design Literature review. Methods This review searched for all relevant peer publications in English for the period 2009 to 2019 in the PubMed database using search terms: “laryngeal stenosis,” “vocal fold scarring,” and “cytokines.” A thorough investigation of the methods and results of the reviewed studies was performed. Results Comprehensive research in various studies, including analyses of prostaglandin E2 (PGE2), granulocyte‐macrophage colony‐stimulating factor (GM‐CSF), hepatocyte growth factor (HGF), basic fibroblast growth factor (bFGF), transforming growth factor‐β3 (TGF‐β3), and interleukin‐10 (IL‐10), supports cytokine therapy for VF scarring and laryngeal stenosis to some extent. A few clinical studies on this topic support the conclusion that HGF and bFGF can be selected as effective drugs, and no serious side effects were found. Conclusions This review describes the potential of cytokines for modulating the process of VF fibrogenesis, although cytokines are still an unproven treatment method. As no ideal drugs exist, cytokines may be considered the candidate treatment for preventing VF fibrogenesis. Laryngoscope, 131:139–145, 2021

show abstract

A tissue-specific, injectable acellular gel for the treatment of chronic vocal fold scarring

Cited by 16 publications

References 82 publications

Porcine Vocal Fold Lamina Propria-Derived Biomaterials Modulate TGF-β1-Mediated Fibroblast Activation in Vitro

Porcine Vocal Fold Lamina Propria-Derived Biomaterials Modulate TGF-β1-Mediated Fibroblast Activation in Vitro

Progress in Vocal Fold Regenerative Biomaterials: An Immunological Perspective

The Role of Cytokines in Modulating Vocal Fold Fibrosis: A Contemporary Review

Contact Info

Product

Resources

About