A tomato ER-type Ca2+-ATPase, LCA1, has a low thapsigargin-sensitivity and can transport manganese

Johnson, Neil A.; Liu, Fengli; Weeks, Phillip D.; Hentzen, Audrey E.; Kruse, Hilary P.; Parker, Jennifer J.; Laursen, Mette; Nissen, Poul; Costa, Charles J.; Gatto, Craig

doi:10.1016/j.abb.2008.11.010

Cited by 23 publications

(19 citation statements)

References 59 publications

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“…The finding that M535L protects K616 from excess Mn 2+ suggests that the viral protein transports manganese in addition to calcium. This behaviour differs from other IIB type Ca 2+ pumps and resembles IIA Ca 2+ pumps ECA1 or ECA3 from A. thaliana or LCA1 from tomato that confer tolerance to Mn 2+ (Wu et al , 2002; Mills et al , 2008; Johnson et al , 2009). However, in contrast to ECA1 and ECA3, M535L does not reverse the toxic effect of Zn 2+ (results not shown).…”

Section: Resultsmentioning

confidence: 71%

“…In plants that lack SPCA pumps, only Ca 2+ -ATPases belonging to type IIA subgroup, such as ECA1 and ECA3 from A. thaliana and LCA1 from tomato, function as Ca 2+ /Mn 2+ pumps (Wu et al , 2002; Mills et al , 2008; Li et al , 2008; Johnson et al , 2009). The best characterized Ca 2+ /Mn 2+ -transporting pump is PMR1, a yeast protein localized in the Golgi apparatus.…”

Section: Discussionmentioning

confidence: 99%

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A functional calcium-transporting ATPase encoded by chlorella viruses

Bonza

Martin

Kang

et al. 2010

Journal of General Virology

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Calcium-transporting ATPases (Ca2+ pumps) are major players in maintaining calcium homeostasis in the cell and have been detected in all cellular organisms. Here, we report the identification of two putative Ca2+ pumps, M535L and C785L, encoded by chlorella viruses MT325 and AR158, respectively, and the functional characterization of M535L. Phylogenetic and sequence analyses place the viral proteins in group IIB of P-type ATPases even though they lack a typical feature of this class, a calmodulin-binding domain. A Ca2+ pump gene is present in 45 of 47 viruses tested and is transcribed during virus infection. Complementation analysis of the triple yeast mutant K616 confirmed that M535L transports calcium ions and, unusually for group IIB pumps, also manganese ions. In vitro assays show basal ATPase activity. This activity is inhibited by vanadate, but, unlike that of other Ca2+ pumps, is not significantly stimulated by either calcium or manganese. The enzyme forms a 32P-phosphorylated intermediate, which is inhibited by vanadate and not stimulated by the transported substrate Ca2+, thus confirming the peculiar properties of this viral pump. To our knowledge this is the first report of a functional P-type Ca2+-transporting ATPase encoded by a virus.

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Section: Resultsmentioning

confidence: 71%

Section: Discussionmentioning

confidence: 99%

A functional calcium-transporting ATPase encoded by chlorella viruses

Bonza

Martin

Kang

et al. 2010

Journal of General Virology

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“…Plant P-type Ca 2+ -ATPases are divided into two groups, 2A and 2B, whereby the first one does not contain an N-terminal autoinhibitory domain (Johnson et al, 2009). Those P 2A -type Ca 2+ -ATPases have also a role in Mn 2+ transport.…”

Section: Manganese Transport Proteins In Plantsmentioning

confidence: 99%

Manganese in Plants: From Acquisition to Subcellular Allocation

et al. 2020

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Manganese (Mn) is an important micronutrient for plant growth and development and sustains metabolic roles within different plant cell compartments. The metal is an essential cofactor for the oxygen-evolving complex (OEC) of the photosynthetic machinery, catalyzing the water-splitting reaction in photosystem II (PSII). Despite the importance of Mn for photosynthesis and other processes, the physiological relevance of Mn uptake and compartmentation in plants has been underrated. The subcellular Mn homeostasis to maintain compartmented Mn-dependent metabolic processes like glycosylation, ROS scavenging, and photosynthesis is mediated by a multitude of transport proteins from diverse gene families. However, Mn homeostasis may be disturbed under suboptimal or excessive Mn availability. Mn deficiency is a serious, widespread plant nutritional disorder in dry, well-aerated and calcareous soils, as well as in soils containing high amounts of organic matter, where bio-availability of Mn can decrease far below the level that is required for normal plant growth. By contrast, Mn toxicity occurs on poorly drained and acidic soils in which high amounts of Mn are rendered available. Consequently, plants have evolved mechanisms to tightly regulate Mn uptake, trafficking, and storage. This review provides a comprehensive overview, with a focus on recent advances, on the multiple functions of transporters involved in Mn homeostasis, as well as their regulatory mechanisms in the plant's response to different conditions of Mn availability.

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“…A tomato ECA, LCA1 (Lycopersicon esculentum Ca 2+ -ATPases), shows high amino acid sequence similarity to ECA2 (77%) in comparison to ECA1 and ECA3 (Pittman et al, 1999). LCA1 can also complement the pmr1 yeast mutant, suggesting that it is also an endocompartment-localized Mn 2+ pump (Johnson et al, 2009). Additional study of ECAs in A. thaliana , rice and other crop species is required to clarify their role in Mn homeostasis in planta .…”

Section: Manganese Transportersmentioning

confidence: 99%

Mn-euvering manganese: the role of transporter gene family members in manganese uptake and mobilization in plants

2014

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Manganese (Mn), an essential trace element, is important for plant health. In plants, Mn serves as a cofactor in essential processes such as photosynthesis, lipid biosynthesis and oxidative stress. Mn deficient plants exhibit decreased growth and yield and are more susceptible to pathogens and damage at freezing temperatures. Mn deficiency is most prominent on alkaline soils with approximately one third of the world's soils being too alkaline for optimal crop production. Despite the importance of Mn in plant development, relatively little is known about how it traffics between plant tissues and into and out of organelles. Several gene transporter families have been implicated in Mn transport in plants. These transporter families include NRAMP (natural resistance associated macrophage protein), YSL (yellow stripe-like), ZIP (zinc regulated transporter/iron-regulated transporter [ZRT/IRT1]-related protein), CAX (cation exchanger), CCX (calcium cation exchangers), CDF/MTP (cation diffusion facilitator/metal tolerance protein), P-type ATPases and VIT (vacuolar iron transporter). A combination of techniques including mutant analysis and Synchrotron X-ray Fluorescence Spectroscopy can assist in identifying essential transporters of Mn. Such knowledge would vastly improve our understanding of plant Mn homeostasis.

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A tomato ER-type Ca2+-ATPase, LCA1, has a low thapsigargin-sensitivity and can transport manganese

Cited by 23 publications

References 59 publications

A functional calcium-transporting ATPase encoded by chlorella viruses

A functional calcium-transporting ATPase encoded by chlorella viruses

Manganese in Plants: From Acquisition to Subcellular Allocation

Mn-euvering manganese: the role of transporter gene family members in manganese uptake and mobilization in plants

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