A tool set to allow rapid screening of dog families with PRA for association with candidate genes

Abstract

This tool set was used quickly and efficiently to narrow down 45 candidate genes for recessively inherited PRA in two types of dogs with known mutations and one type of dog with an unknown mutation.

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“…A previously developed panel of microsatellite markers flanking 45 PRA candidate genes was used to initially screen three affected SWDs for an association between the markers and the phenotype. 5 Genes were excluded if (a) one affected dog was heterozygous for both markers and/or (b) more than one affected dog was heterozygous for one marker. Allele sizes had to be more than one repeat size different to be considered separate alleles, (see [5][6][7] for detailed descriptions of exclusion analysis).…”

“…5 Genes were excluded if (a) one affected dog was heterozygous for both markers and/or (b) more than one affected dog was heterozygous for one marker. Allele sizes had to be more than one repeat size different to be considered separate alleles, (see [5][6][7] for detailed descriptions of exclusion analysis). After the first round of exclusions, the remaining unexcluded genes were screened with two additional affected SWDs and three phenotypically normal SWDs (dam, sire, and sibling).…”

“…A marker panel consisting of 90 microsatellites flanking 45 PRA candidate genes was utilized to rapidly screen these genes for association with PRA in the affected SWDs. 5 Initially, three affected dogs were genotyped in the panel and 39/45 genes were excluded (data not shown). A gene was excluded if there was at least 1 dog genotyped with an allele size difference greater than ±1 repeat in at least 1 of the markers.…”