A trace amine, tyramine, functions as a neuromodulator in Drosophila melanogaster

Nagaya, Yuki; Kutsukake, Mayako; Chigusa, Sadao I.; Komatsu, Akira

doi:10.1016/s0304-3940(02)00596-7

Cited by 110 publications

(107 citation statements)

References 18 publications

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“…These observations were extended by Nagaya et al (2002) who studied the actions of TYR and OCT in a Drosophila mutant known as honoka (hono). This mutant fly carries a Pelement inserted 100 bases upstream of the OCT/TYR receptor gene cloned by Arakawa et al (1990) and Saubou et al (1990).…”

Section: Invertebrate Trace Amine-activated Receptorsmentioning

confidence: 92%

“…It has long been appreciated that TAs, in particular OCT and TYR, are likely neurotransmitters, and not neuromodulators, that substitute for NE in invertebrates Saavedra & Axelrod, 1976;Roberston & Juorio, 1976;Axelrod & Saavedra, 1977;Evans & O'Shea;1977;Roeder, 1999) although Nagaya et al, (2002) found TYR to function as a neuromodulator in Drosophila melanogaster and Donini et al (2004) reported evidence that TYR functions as both a neurotransmitter and neuromodulator in the locust oviduct.…”

Section: 42mentioning

confidence: 99%

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Trace amine-associated receptor 1—Family archetype or iconoclast?

Grandy

2007

Pharmacology & Therapeutics

178

211

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Interest has recently been rekindled in receptors that are activated by low molecular weight, noncatecholic, biogenic amines that are typically found as trace constituents of various vertebrate and invertebrate tissues and fluids. The timing of this resurgent focus on receptors activated by the 'trace amines' (TAs) β-phenylethylamine (PEA), tyramine (TYR), octopamine (OCT), synephrine (SYN), and tryptamine (TRYP) is the direct result of two publications that appeared in 2001 describing the cloning of a novel G protein-coupled receptor (GPCR) referred to by their discoverers as TA1 (Borowsky et al., 2001) and TAR1 (Bunzow et al., 2001). When heterologously expressed in Xenopus laevis oocytes and various eukaryotic cell lines recombinant rodent and human TA receptors dosedependently couple to the stimulation of cAMP production. Structure-activity profiling based on this functional response has revealed that in addition to the TAs, other biologically active compounds containing a 2 carbon aliphatic side chain linking an amino group to at least one benzene ring are potent and efficacious TA receptor agonists with amphetamine, methamphetamine, 3-iodothyronamine, thyronamine, and dopamine among the most notable. Almost 100 years after the search for TA receptors began numerous TA1/TAR1-related sequences, now called Trace AmineAssociated Receptors (TAARs), have been identified in the genome of every species of vertebrate examined to date. Consequently, even though heterologously expressed TAAR1 fits the pharmacological criteria established for a bona fide TA receptor a major challenge for those working in the field is to discern the in vivo pharmacology and physiology of each purported member of this extended family of GPCRs. Only then will it be possible to establish whether TAAR1 is the family archetype or an iconoclast.

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Section: Invertebrate Trace Amine-activated Receptorsmentioning

confidence: 92%

Section: 42mentioning

confidence: 99%

Trace amine-associated receptor 1—Family archetype or iconoclast?

Grandy

2007

Pharmacology & Therapeutics

178

211

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“…TA-and OA-specific neurons, receptors, signaling pathways, and phenotypes have been described in both insects (Saudou et al, 1990) and nematodes . In insects, TA and OA have opposite effects on a variety of behaviors, including locomotion, excitatory junction potentials in muscle strips, and chloride ion conductance across Malpighian tubules in Drosophila (Nagaya et al, 2002;Blumenthal, 2003;Saraswati et al, 2003). In general, insect GPCRs with a preference for TA couple to a decrease in cAMP levels, whereas GPCRs with a preference for OA couple to an elevation in cAMP levels (Roeder, 2005).…”

Section: Figurementioning

confidence: 99%

Tyramine and Octopamine Independently Inhibit Serotonin-Stimulated Aversive Behaviors inCaenorhabditis elegansthrough Two Novel Amine Receptors

Wragg¹,

Hapiak²,

Miller³

et al. 2007

J. Neurosci.

115

146

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Biogenic amines modulate key behaviors in both vertebrates and invertebrates. In Caenorhabditis elegans, tyramine (TA) and octopamine (OA) inhibit aversive responses to 100%, but not dilute (30%) octanol. TA and OA also abolish food-and serotonin-dependent increases in responses to dilute octanol in wild-type but not tyra-3(ok325) and f14d12.6(ok371) null animals, respectively, suggesting that TA and OA modulated responses to dilute octanol are mediated by separate, previously uncharacterized, G-protein-coupled receptors. TA and OA are high-affinity ligands for TYRA-3 and F14D12.6, respectively, based on their pharmacological characterization after heterologous expression. f14d12.6::gfp is expressed in the ASHs, the neurons responsible for sensitivity to dilute octanol, and the sra-6-dependent expression of F14D12.6 in the ASHs is sufficient to rescue OA sensitivity in f14d12.6(ok371) null animals. In contrast, tyra-3::gfp appears not to be expressed in the ASHs, but instead in other neurons, including the dopaminergic CEP/ADEs. However, although dopamine (DA) also inhibits 5-HT-dependent responses to dilute octanol, TA still inhibits in dop-2; dop-1; dop-3 animals that do not respond to DA and cat-2(tm346) and Pdat-1::ICE animals that lack significant dopaminergic signaling, suggesting that DA is not an intermediate in TA inhibition. Finally, responses to TA and OA selectively desensitize after preexposure to the amines. Our data suggest that although tyraminergic and octopaminergic signaling yield identical phenotypes in these olfactory assays, they act independently through distinct receptors to modulate the ASH-mediated locomotory circuit and that C. elegans is a useful model to study the aminergic modulation of sensory-mediated locomotory behaviors.

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“…While all octopaminergic neurons are by definition also tyraminergic, there have been tyraminergic neurons described in D. melanogaster, L. migratoria and C. elegans that do not contain OA (Nagaya et al, 2002;Donini and Lange, 2004;Alkema et al, 2005, respectively). Furthermore, several studies have demonstrated that tyramine has physiological effects that are independent, and often antagonistic to, OA (see Roeder et al, 2003).…”

Section: Cloning and Sequence Analysis Of The Msoarmentioning

confidence: 99%

The cloning of one putative octopamine receptor and two putative serotonin receptors from the tobacco hawkmoth, Manduca sexta

Dacks

Christensen

et al. 2006

Insect Biochemistry and Molecular Biology

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Serotonin and octopamine (OA) are biogenic amines that are active throughout the nervous systems of insects, affecting sensory processing, information coding and behavior. As an initial step towards understanding the modulatory roles of these amines in olfactory processing we cloned two putative serotonin receptors (Ms5HT1A and Ms5HT1B) and one putative OA (MsOAR) receptor from the moth Manduca sexta. Ms5HT1A and Ms5HT1B were both similar to 5HT1-type receptors but differed from each other in their N-terminus and 3rd cytoplasmic loop. Ms5HT1A was nearly identical to a serotonin receptor from Heliothis virescens and Ms5HT1B was almost identical to a serotonin receptor from Bombyx mori. The sequences for homologs of Ms5HT1A from B. mori and Ms5HT1B from H. virescens were also obtained, suggesting that the Lepidoptera likely have at least two serotonin receptors. The MsOAR shares significant sequence homology with pharmacologically characterized OA receptors, but less similarity to putative OA/tyramine receptors from the moths B. mori and H. virescens. Using the MsOAR sequence, fragments encoding putative OA receptors were obtained from B. mori and H. virescens, suggesting that MsOAR is the first OA receptor cloned from a lepidopteran.

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A trace amine, tyramine, functions as a neuromodulator in Drosophila melanogaster

Cited by 110 publications

References 18 publications

Trace amine-associated receptor 1—Family archetype or iconoclast?

Trace amine-associated receptor 1—Family archetype or iconoclast?

Tyramine and Octopamine Independently Inhibit Serotonin-Stimulated Aversive Behaviors inCaenorhabditis elegansthrough Two Novel Amine Receptors

The cloning of one putative octopamine receptor and two putative serotonin receptors from the tobacco hawkmoth, Manduca sexta

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