A Transcriptomic Analysis of Stylo [Stylosanthes guianensis (Aubl.) Sw.] Provides Novel Insights Into the Basis of Salinity Tolerance

Liu, Yiming; Kong, Danyu; Yang, Hubiao; Douxchamps, Sabine; Atieno, Mary; Xu, Bin; Wang, Wenqiang; Liu, Guodao

doi:10.3389/fsufs.2022.725656

Cited by 4 publications

(3 citation statements)

References 24 publications

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“…This study introduced the first nuclear genome assembly of a species of the genus Stylosanthes . Despite the availability of transcriptomic studies [ 13 , 14 , 15 , 16 , 17 ], only one initiative has addressed the genome (in that case, from chloroplast [ 18 ]) of representatives of the mentioned genus.…”

Section: Discussionmentioning

confidence: 99%

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Bridging the Gap: Combining Genomics and Transcriptomics Approaches to Understand Stylosanthes scabra, an Orphan Legume from the Brazilian Caatinga

Ferreira-Neto,

da Silva,

Binneck

et al. 2023

Plants

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Stylosanthes scabra is a scientifically orphaned legume found in the Brazilian Caatinga biome (a semi-arid environment). This work utilized omics approaches to investigate some ecophysiological aspects of stress tolerance/resistance in S. scabra, study its genomic landscape, and predict potential metabolic pathways. Considering its high-confidence conceptual proteome, 1694 (~2.6%) proteins were associated with resistance proteins, some of which were found in soybean QTL regions that confer resistance to Asian soybean rust. S. scabra was also found to be a potential source of terpenes, as biosynthetic gene clusters associated with terpene biosynthesis were identified in its genome. The analysis revealed that mobile elements comprised approximately 59% of the sequenced genome. In the remaining 41% of the sections, some of the 22,681 protein-coding gene families were categorized into two informational groups: those that were specific to S. scabra and those that expanded significantly compared to their immediate ancestor. Biological process enrichment analyses indicated that these gene families play fundamental roles in the adaptation of S. scabra to extreme environments. Additionally, phylogenomic analysis indicated a close evolutionary relationship between the genera Stylosanthes and Arachis. Finally, this study found a high number (57) of aquaporin-encoding loci in the S. scabra genome. RNA-Seq and qPCR data suggested that the PIP subfamily may play a key role in the species’ adaptation to water deficit conditions. Overall, these results provide valuable insights into S. scabra biology and a wealth of gene/transcript information for future legume omics studies.

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Section: Discussionmentioning

confidence: 99%

“…To date, this is the only study to present transcriptomic data for S. scabra . For the genus Stylosanthes, there are reports of only four more studies [ 14 , 15 , 16 , 17 ] addressing high-throughput gene expression studies, all on Stylosanthes guianensis .…”

Section: Introductionmentioning

confidence: 99%

Bridging the Gap: Combining Genomics and Transcriptomics Approaches to Understand Stylosanthes scabra, an Orphan Legume from the Brazilian Caatinga

Ferreira-Neto,

da Silva,

Binneck

et al. 2023

Plants

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show abstract

“…Reyan 2]; Jia et al, 2020 [ S. guianensis ]; Jiang et al, 2021 [ S. guianensis cv. Reyan 2]; and Liu et al, 2022 [ S. guianensis [Aubl.] Sw.]) addressing high‐throughput gene expression studies for the Stylosanthes genus.…”

Section: Introductionmentioning

confidence: 99%

Dehydration response in Stylosanthes scabra: Transcriptional, biochemical, and physiological modulations

Ferreira‐Neto

Araújo

Silva

et al. 2022

Physiologia Plantarum

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Stylosanthes scabra, popularly known as stylo, is native to the Brazilian Caatinga semiarid region and stands out as a drought‐tolerant shrub forage crop. This work provides information about the plant response during the first 48 h of water deficit, followed by a rehydration treatment. Besides root transcriptomics data, 13 physiological or biochemical parameters were scrutinized. Additionally, RNA‐Seq annotated transcripts not associated with the “Viridiplantae” clade were taxonomically categorized. It was found that S. scabra quickly perceives and recovers from the oscillations of the imposed water regime. Physiologically, mechanisms that minimize evapotranspiration or protect the photosynthetic apparatus stood out. Biochemically, it was found that the root tissue invests in synthesizing compounds that can act as osmolytes (proline and sugars), emphasizing the importance of osmoregulation to water deficit acclimation. Consistently, transcriptome and qPCR analyses showed that a set of enriched biological processes with upregulated (UR) transcripts were involved in protective functions against reactive oxygen species or encoding enzymes of important metabolic pathways, which might contribute to S. scabra response to water deficit. Additionally, several UR kinases and transcription factors were identified. Finally, in an innovative approach, some naturally occurring microbial groups (such as Schizosaccharomyces, Bradyrhizobium, etc.) were identified in the S. scabra roots. This study reveals insights into the physiological, biochemical, and molecular mechanisms underlying the S. scabra response to water deficit and provides candidate genes that may be useful in developing drought‐tolerant crop varieties through biotechnological applications.

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Harnessing genomic technologies for one health solutions in the tropics

Calcino,

Cooke,

Cowman

et al. 2024

Global Health

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A Transcriptomic Analysis of Stylo [Stylosanthes guianensis (Aubl.) Sw.] Provides Novel Insights Into the Basis of Salinity Tolerance

Cited by 4 publications

References 24 publications

Bridging the Gap: Combining Genomics and Transcriptomics Approaches to Understand Stylosanthes scabra, an Orphan Legume from the Brazilian Caatinga

Bridging the Gap: Combining Genomics and Transcriptomics Approaches to Understand Stylosanthes scabra, an Orphan Legume from the Brazilian Caatinga

Dehydration response in Stylosanthes scabra: Transcriptional, biochemical, and physiological modulations

Harnessing genomic technologies for one health solutions in the tropics

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