A transgenic Cre mouse line for the study of cortical and hippocampal development

Zhou, Wei; Zhang, Ying; Li, Yiping; Wei, Yu‐Sheng; Liu, Guang; Liu, De-Pei; Pleasure, Samuel J.; Xie, Wei; Zhao, Chunjie

doi:10.1002/dvg.20611

Cited by 5 publications

(6 citation statements)

References 23 publications

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“…Male mice at 8–12 weeks of age were used for ribosome pull-down and RNA-seq experiments. The following mouse strains were used: RiboTag ( Sanz et al, 2009 ), Adv Cre ( Zhou et al, 2010 ; da Silva et al, 2011 ), Isl1 Cre ( Yang et al, 2006 ), Dhh Cre ( Jaegle et al, 2003 ), Runx3 Cre ( Levanon et al, 2011 ), PGK Cre ( Lallemand et al, 1998 ), and ApoE null ( Piedrahita et al, 1992 ). Wistar rats were used for proteomics analyses and C57BL/6 YFP16 mice ( Feng et al, 2000 ) for functional validation of selected candidates.…”

Section: Methodsmentioning

confidence: 99%

Translatome Regulation in Neuronal Injury and Axon Regrowth

Rozenbaum

Rajman

Rishal

et al. 2018

eNeuro

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Transcriptional events leading to outgrowth of neuronal axons have been intensively studied, but the role of translational regulation in this process is not well understood. Here, we use translatome analyses by ribosome pull-down and protein synthesis characterization by metabolic isotopic labeling to study nerve injury and axon outgrowth proteomes in rodent dorsal root ganglia (DRGs) and sensory neurons. We identify over 1600 gene products that are primarily translationally regulated in DRG neurons after nerve injury, many of which contain a 5’UTR cytosine-enriched regulator of translation (CERT) motif, implicating the translation initiation factor Eif4e in the injury response. We further identified approximately 200 proteins that undergo robust de novo synthesis in the initial stages of axon growth. ApoE is one of the highly synthesized proteins in neurons, and its receptor binding inhibition or knockout affects axon outgrowth. These findings provide a resource for future analyses of the role of translational regulation in neuronal injury responses and axon extension.

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Section: Methodsmentioning

confidence: 99%

Translatome Regulation in Neuronal Injury and Axon Regrowth

Rozenbaum

Rajman

Rishal

et al. 2018

eNeuro

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“…Wnt3, Wnt5a, Wnt7a, and Wnt7b are all present in the mammalian hippocampus, although Wnt3 is not widely expressed (Rosso et al 2005; Davis et al 2008). The Wnt receptors Fzd3, Fzd5, Fzd8, and Fzd9 are also all expressed in the postnatal hippocampus (Davis et al 2008; Zhou et al 2010). These Wnts and Fzds are therefore natural candidates for considering the role of Wnt signaling at mammalian CNS synapses.…”

Section: Wnts and Synapse Formation In The Mammalian Cnsmentioning

confidence: 99%

Synaptic Wnt signaling—a contributor to major psychiatric disorders?

Okerlund

Cheyette

2011

J Neurodevelop Disord

132

100

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Wnt signaling is a key pathway that helps organize development of the nervous system. It influences cell proliferation, cell fate, and cell migration in the developing nervous system, as well as axon guidance, dendrite development, and synapse formation. Given this wide range of roles, dysregulation of Wnt signaling could have any number of deleterious effects on neural development and thereby contribute in many different ways to the pathogenesis of neurodevelopmental disorders. Some major psychiatric disorders, including schizophrenia, bipolar disorder, and autism spectrum disorders, are coming to be understood as subtle dysregulations of nervous system development, particularly of synapse formation and maintenance. This review will therefore touch on the importance of Wnt signaling to neurodevelopment generally, while focusing on accumulating evidence for a synaptic role of Wnt signaling. These observations will be discussed in the context of current understanding of the neurodevelopmental bases of major psychiatric diseases, spotlighting schizophrenia, bipolar disorder, and autism spectrum disorder. In short, this review will focus on the potential role of synapse formation and maintenance in major psychiatric disorders and summarize evidence that defective Wnt signaling could contribute to their pathogenesis via effects on these late neural differentiation processes.

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“…Lgr5-EGFP-IRES-creERT2 mice (Stock #008875, Jackson Laboratory) and Rosa26-tdTomato reporter mice (Stock #007914, Jackson Laboratory) of both sexes were used in the experiments (Madisen et al, 2010). The Fzd9-CreER mice were a gift from Prof Chunjie Zhao from Southeast University (Zhou et al, 2010). We performed all animal procedures according to protocols that were approved by the Animal Care and Use Committee of Southeast University and that were consistent with the National Institute of Health’s Guide for the Care and Use of Laboratory Animals.…”

Section: Methodsmentioning

confidence: 99%

“…Frizzled-9 (Fzd9) plays crucial roles in brain development, neuromuscular junction assembly, new bone formation, and tumor suppression (Winn et al, 2005; Zhao et al, 2005; Albers et al, 2011; Avasarala et al, 2013b; Heilmann et al, 2013; Aviles et al, 2014; Ramírez et al, 2016). In the brain, Fzd9 is selectively expressed in both the developing and adult hippocampus and is used to mark neural stem cells together with other markers (Van Raay et al, 2001; Zhao and Pleasure, 2004, 2005; Pollard et al, 2008; Trubiani et al, 2010; Zhou et al, 2010; Pedersen et al, 2012; Tian et al, 2012). In human bone marrow and chorionic placenta-derived mesenchymal stem cells, Fzd9 is also expressed on the cell surface and is used as a marker to isolate these cells (Battula et al, 2007, 2008; Buhring et al, 2007; Trubiani et al, 2010; Tran et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Frizzled-9+ Supporting Cells Are Progenitors for the Generation of Hair Cells in the Postnatal Mouse Cochlea

Zhang

Liu

Dong

et al. 2019

Front. Mol. Neurosci.

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Lgr5+ cochlear supporting cells (SCs) have been reported to be hair cell (HC) progenitor cells that have the ability to regenerate HCs in the neonatal mouse cochlea, and these cells are regulated by Wnt signaling. Frizzled-9 (Fzd9), one of the Wnt receptors, has been reported to be used to mark neuronal stem cells in the brain together with other markers and mesenchymal stem cells from human placenta and bone marrow. Here we used Fzd9-CreER mice to lineage label and trace Fzd9+ cells in the postnatal cochlea in order to investigate the progenitor characteristic of Fzd9+ cells. Lineage labeling showed that inner phalangeal cells (IPhCs), inner border cells (IBCs), and third-row Deiters’ cells (DCs) were Fzd9+ cells, but not inner pillar cells (IPCs) or greater epithelial ridge (GER) cells at postnatal day (P)3, which suggests that Fzd9+ cells are a much smaller cell population than Lgr5+ progenitors. The expression of Fzd9 progressively decreased and was too low to allow lineage tracing after P14. Lineage tracing for 6 days in vivo showed that Fzd9+ cells could also generate similar numbers of new HCs compared to Lgr5+ progenitors. A sphere-forming assay showed that Fzd9+ cells could form spheres after sorting by flow cytometry, and when we compared the isolated Fzd9+ cells and Lgr5+ progenitors there were no significant differences in sphere number or sphere diameter. In a differentiation assay, the same number of Fzd9+ cells could produce similar amounts of Myo7a+ cells compared to Lgr5+ progenitors after 10 days of differentiation. All these data suggest that the Fzd9+ cells have a similar capacity for proliferation, differentiation, and HC generation as Lgr5+ progenitors and that Fzd9 can be used as a more restricted marker of HC progenitors.

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A transgenic Cre mouse line for the study of cortical and hippocampal development

Cited by 5 publications

References 23 publications

Translatome Regulation in Neuronal Injury and Axon Regrowth

Translatome Regulation in Neuronal Injury and Axon Regrowth

Synaptic Wnt signaling—a contributor to major psychiatric disorders?

Frizzled-9+ Supporting Cells Are Progenitors for the Generation of Hair Cells in the Postnatal Mouse Cochlea

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