“…After stopping confocal analysis, allantoides were fixed and immunolabeled with anti-PECAM-1 and phospho-Histone 3 antibodies as described previously (Baumer et al, 2006). domains 1-8 of VE-PTP, pAb D17 against the extracellular membrane proximal fibronectin type III-like domain of VE-PTP, mAb 3G1 against Tie-2 ( Koblizek et al, 1997), mAb against human Tie-2 (Millipore), pAb against Tie-1 (Santa Cruz Biotechnology, Inc.), pAb against phospho-Tie-2-Tyr992, pAb against phospho-Erk1/2-Thr202/Tyr204 pAb and mAb against Erk1/2, pAb against phospho-Akt-Ser473, pAb against Akt (Cell Signaling Technology), mAb 11D4.1 and pAb C5 against murine VE-cadherin (Gotsch et al, 1997), pAb against human VE-cadherin (Santa Cruz Biotechnology, Inc.), mAb 1G8 against mouse ESAM (Nasdala et al, 2002), pAb against human ESAM , mAb against PECAM-1 1G5.1 and 5D2.6 (Wegmann et al, 2006), mAb against plakoglobin (BD Biosciences), mAb 4G10 against phosphotyrosine (Millipore), and mAb and pAb against phospho-Histone H3-Ser10 (Millipore). Secondary antibodies were purchased from Dianova; Alexa 488-, Alexa 633-, and Alexa 568-coupled antibodies from Invitrogen; and DyLight680 and DyLight800-coupled antibodies from Thermo Fisher Scientific.…”