A truncated peptide Sp-NPFin from the neuropeptide FII SpNPFII of Scylla paramamosain exhibiting potent antimicrobial activity

Zhang, Hua; Yang, Ying; Zhu, Xuewu; Liu, Yangzhou; Chen, Fangyi; Wang, Kejian

doi:10.1016/j.aquaculture.2020.736145

Cited by 6 publications

(4 citation statements)

References 56 publications

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“…Our current study revealed that LJ-hep2 (66–86) possessed strong antimicrobial activities against these multi-drug resistant bacteria isolated from clinical samples. This result is consistent with the previous findings that the peptides Sp-NPFin [ 35 ] from Scylla paramamosain and AS-hepc3 (48–56) [ 36 ] from A. schlegelii effectively inhibited the growth of several multi-drug resistant bacteria. Currently, AMPs are being explored as alternative therapy to tackle infections caused by multidrug-resistant bacteria [ 37 ].…”

Section: Discussionsupporting

confidence: 93%

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The Antimicrobial Peptide LJ-hep2 from Lateolabrax japonicus Exerting Activities against Multiple Pathogenic Bacteria and Immune Protection In Vivo

Gong

Zhang

et al. 2022

Marine Drugs

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Hepcidin is widely present in many kinds of fish and is an important innate immune factor. A variety of HAMP2-type hepcidins have strong antimicrobial activity and immunomodulatory functions and are expected to be developed as substitutes for antibiotics. In this study, the antimicrobial activity of Hepc2 from Japanese seabass (Lateolabrax japonicus) (designated as LJ-hep2) was investigated using its recombinant precursor protein (rLJ-hep2) expressed in Pichia pastoris and a chemically synthesized mature peptide (LJ-hep2(66–86)). The results showed that both rLJ-hep2 and synthetic LJ-hep2(66–86) displayed broad antimicrobial spectrum with potent activity against gram-negative and gram-positive bacteria, and fungi. Especially, LJ-hep2(66–86) had stronger antimicrobial activity and exhibited potent activity against several clinically isolated multidrug-resistant bacteria, including Acinetobacter baumannii, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and Enterococcus faecium. Moreover, LJ-hep2(66–86) exerted rapid bactericidal kinetic (killed tested bacteria within 2 h), induced significant morphological changes and promoted agglutination of E. coli, P. aeruginosa and Aeromonas hydrophila. The activity of LJ-hep2(66–86) against E. coli, P. aeruginosa and A. hydrophila was stable and remained active when heated for 30 min. In addition, LJ-hep2(66–86) exhibited no cytotoxicity to the mammalian cell line HEK293T and fish cell lines (EPC and ZF4). In vivo study showed that LJ-hep2(66–86) could improve the survival rate of marine medaka (Oryzias melastigma) by about 40% under the challenge of A. hydrophila, indicating its immunoprotective function. Taken together, both rLJ-hep2 and LJ-hep2(66–86) have good prospects to be used as potential antimicrobial agents in aquaculture and medicine in the future.

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Section: Discussionsupporting

confidence: 93%

“…The time-killing kinetics assay was performed as previously described [ 35 , 49 ]. The bacteria E. coli , P. aeruginosa and A. hydrophila were harvested and adjusted to 3.3 × 10 4 CFU/mL as described for the antimicrobial assay.…”

Section: Methodsmentioning

confidence: 99%

The Antimicrobial Peptide LJ-hep2 from Lateolabrax japonicus Exerting Activities against Multiple Pathogenic Bacteria and Immune Protection In Vivo

Gong

Zhang

et al. 2022

Marine Drugs

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“…Further studies are needed to obtain its recombinant protein and confirm its immune-related functions in vitro and in vivo. Other novel AMPs, such as Sparamosin 26–54 [ 17 ], Sphistin [ 7 , 18 ], SpHyastatin [ 19 ], Sp-NPFin [ 20 ], etc., including the above AMPs as mentioned, all play an important role in the innate immunity of S. paramamosin and have good prospects for future applications in aquaculture and medicine. Here, in this study, another novel functional gene with high AMP potential, named Spampcin, was characterized from S. paramamosain and its derived truncated peptide Spa31 had been confirmed to exhibit broad-spectrum and potent antibacterial and antifungal activity, and several shortened Spa31-derived peptides (Spa20, Spa22, and Spa14) also had certain antimicrobial activity.…”

Section: Discussionmentioning

confidence: 99%

“…S. aureus and P. aeruginosa were further selected to determine the bactericidal kinetics of Spa31 following a previous report [ 20 ]. Briefly, the final concentration of Spa31 was adjusted to 3 μM or 6 μM (1 × MIC or 1 × MBC for both bacteria).…”

Section: Methodsmentioning

confidence: 99%

A Novel Antimicrobial Peptide Spampcin56–86 from Scylla paramamosain Exerting Rapid Bactericidal and Anti-Biofilm Activity In Vitro and Anti-Infection In Vivo

Jiang

Chen

Zhang

et al. 2022

IJMS

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The abuse of antibiotics leads to the increase of bacterial resistance, which seriously threatens human health. Therefore, there is an urgent need to find effective alternatives to antibiotics, and antimicrobial peptides (AMPs) are the most promising antibacterial agents and have received extensive attention. In this study, a novel potential AMP was identified from the marine invertebrate Scylla paramamosain and named Spampcin. After bioinformatics analysis and AMP database prediction, four truncated peptides (Spa31, Spa22, Spa20 and Spa14) derived from Spampcin were screened, all of which showed potent antimicrobial activity with different antibacterial spectrum. Among them, Spampcin56–86 (Spa31 for short) exhibited strong bactericidal activity against a variety of clinical pathogens and could rapidly kill the tested bacteria within minutes. Further analysis of the antibacterial mechanism revealed that Spa31 disrupted the integrity of the bacterial membrane (as confirmed by scanning electron microscopy observation, NPN, and PI staining assays), leading to bacterial rupture, leakage of cellular contents (such as elevated extracellular ATP), increased ROS production, and ultimately cell death. Furthermore, Spa31 was found to interact with LPS and effectively inhibit bacterial biofilms. The antibacterial activity of Spa31 had good thermal stability, certain ion tolerance, and no obvious cytotoxicity. It is worth noting that Spa31 could significantly improve the survival rate of zebrafish Danio rerio infected with Pseudomonas aeruginosa, indicating that Spa31 played an important role in anti-infection in vivo. This study will enrich the database of marine animal AMPs and provide theoretical reference and scientific basis for the application of marine AMPs in medical fields.

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A novel antimicrobial peptide Scyreptin1-30 from Scylla paramamosain exhibiting potential therapy of Pseudomonas aeruginosa early infection in a mouse burn wound model

Zhang,

An,

Bai

et al. 2023

Biochemical Pharmacology

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A truncated peptide Sp-NPFin from the neuropeptide FII SpNPFII of Scylla paramamosain exhibiting potent antimicrobial activity

Cited by 6 publications

References 56 publications

The Antimicrobial Peptide LJ-hep2 from Lateolabrax japonicus Exerting Activities against Multiple Pathogenic Bacteria and Immune Protection In Vivo

The Antimicrobial Peptide LJ-hep2 from Lateolabrax japonicus Exerting Activities against Multiple Pathogenic Bacteria and Immune Protection In Vivo

A Novel Antimicrobial Peptide Spampcin56–86 from Scylla paramamosain Exerting Rapid Bactericidal and Anti-Biofilm Activity In Vitro and Anti-Infection In Vivo

A novel antimicrobial peptide Scyreptin1-30 from Scylla paramamosain exhibiting potential therapy of Pseudomonas aeruginosa early infection in a mouse burn wound model

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