A. tumefaciens-mediated transient expression as a tool for antigen production for cucurbit yellow stunting disorder virus

Steel, Elspeth; Barker, Ian K.; Danks, C.; Coates, D. M.; Boonham, Neil

doi:10.1016/j.jviromet.2009.09.024

Cited by 8 publications

(4 citation statements)

References 21 publications

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“…However, ELISA has many limitations which restrict its use for accurate detection of plant virus especially when the virus titer is low or the virus strains are closely related [23]. Therefore, nucleic acid based polymerase chain reaction (PCR/RT-PCR) is a method of choice for indexing of different viruses to overcome this problem [18,25].…”

Section: Confirmation Of Ctv By Pcr/rt-pcrmentioning

confidence: 99%

High incidence of citrus tristeza virus in mandarin (Citrus reticulata) in North-East states of India

et al. 2017

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Khasi mandarin is one of the most remunerative crop in North Eastern Hills region of India playing a very critical role in the socio-economic upliftment of the people. The orchards of this region were found to express the typical symptoms of citrus tristeza virus (CTV). An extensive survey of the Khasi mandarin orchard for six North Eastern states of India namely Arunachal Pradesh, Meghalaya, Assam, Nagaland, Sikkim and Tripura, was carried out to establish the identity of these viruses using ELISA and PCR/RT-PCR techniques. Out of 300 Khasi mandarin tree samples collected from the six states, 172 were found to be positive for CTV infection by DAS-ELISA indicating 57.33% overall CTV disease incidence. Results revealed presence of CTV in all the surveyed states showing a maximum incidence of 66.00% in Arunachal Pradesh followed by 62.00% in Assam, 60.00% in Meghalaya and Nagaland, 54.00% in Sikkim and 42.33% in Tripura. Higher CTV concentration was recorded in the age group > 15 years (69.09%) followed by 10-15 (57%) and 5-10 years (43.33%). However, this study, to the best of our knowledge, is the first report for the detection of CTV in Khasi mandarin from Arunachal Pradesh, Nagaland and Tripura and also the first authentic survey of overall disease incidence of CTV Khasi mandarins from the six major mandarin growing North Eastern states of India.

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Section: Confirmation Of Ctv By Pcr/rt-pcrmentioning

confidence: 99%

High incidence of citrus tristeza virus in mandarin (Citrus reticulata) in North-East states of India

et al. 2017

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“…Several studies developed antibodies for CYSDV and CCYV CP expressed in E. coli cells [33,41]. Recently, Steel et al [77] introduced a novel technique to produce CYSDV CP using ''Agrobacterium tumefaciens-mediated transient expression system'' in plants. These antibodies have been used successfully in DAS-ELISA, plate trapped antibody-ELISA (PTA-ELISA), tissue-blot immuno assay (TBIA), dot-blot immuno assay (DBIA), immuno-capture reverse transcription polymerase chain reaction (IC-RT-qPCR) and immuno-electron microscopy (IEM) [33,77].…”

Section: Diagnosismentioning

confidence: 99%

“…Recently, Steel et al [77] introduced a novel technique to produce CYSDV CP using ''Agrobacterium tumefaciens-mediated transient expression system'' in plants. These antibodies have been used successfully in DAS-ELISA, plate trapped antibody-ELISA (PTA-ELISA), tissue-blot immuno assay (TBIA), dot-blot immuno assay (DBIA), immuno-capture reverse transcription polymerase chain reaction (IC-RT-qPCR) and immuno-electron microscopy (IEM) [33,77]. In general, TBIA is a preferred technique for rapid field surveys and may be just slightly less sensitive than RT-PCR [1,82].…”

Section: Diagnosismentioning

confidence: 99%

Whitefly-transmitted criniviruses of cucurbits: current status and future prospects

Abrahamian

Abou‐Jawdah

2013

VirusDis.

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In the past decade, crinviruses have gained interest due to their rapid widespread and destructive nature for cucurbit cultivation. Several members of the genus Crinivirus are considered emerging viruses. Currently, four criniviruses: Beet pseudo-yellows virus, Cucurbit chlorotic yellows virus, Cucurbit yellow stunting disorder virus and Lettuce infectious yellows virus have been reported to infect field-or greenhouse-grown cucurbits. Apart from their cucurbit hosts, criniviruses infect other cash crops and weeds. Criniviruses are exclusively transmitted by whiteflies. The virion titer and the vector genus or species complex are predominant factors affecting virus transmission. These criniviruses maintain genetic stability with limited intra-species variability. They share similar core genome structure and replication strategies with some variations in the non-core proteins and downstream replication processes. Management of the diseases induced by criniviruses relies on integrated disease management strategies and on resistant varieties, when available. This review will cover their epidemiology, molecular biology, detection and management.

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“…(Hourani & Abou-Jawdah, 2003;M. Jacquemond et al, 2009;Kubota et al, 2011;Steel et al, 2010). In most reports, due to the symptoms similarity between CCYV and CYSDV, DAS-ELISA was used for a first evaluation and RT-PCR for an accurate detection of both CYSDV and CCYV Al-Saleh et al, 2015;.…”

Section: Diagnostic Methodsmentioning

confidence: 99%

First report of Cucurbit Chlorotic Yellows Virus (CCYV) and Tomato Leaf Curl New Delhi Virus (ToLCNDV) in Algeria and lack of evidence for seed transmission of ToLCNDV in melon and pumpkin.

Kheireddine

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Emerging plant viruses in the Mediterranean Basin have arisen in the last decades becoming an important threat to crop production in this region. The spread of new viral diseases has become one of the main factors limiting agronomic and economic yields of cultivated species of Cucurbitaceae family, the second in importance after Solanaceae. A wide cluster of elements modulates and favors the emergence of these pathogens, including biological, ecological, social, and cultural factors. Global warming favors vector movement to new geographies, while global commercial networks lead to germplasm trade among countries, allowing the long-distance dissemination of viruses. Moreover, the intensive agricultural model, in which prevail genetically homogeneous cultivars, prompts pathogens outbreaks within new geographic areas at a fast rate.One of the key components to develop control strategies, and secure horticultural production, is to identify the type and distribution of viruses present in crops. However, such information in Algeria is scarce and very limited. The objective of this Doctoral Thesis was to identify and determine the prevalence and distribution of the most common viruses on cultivated cucurbit plants (zucchini, cucumber, and melon), and get information about their genetic variability and mode of dispersion.In this work, cucurbit chlorotic yellows virus (CCYV) was first detected infecting cucumber and zucchini in Algeria. CCYV (genus Crinivirus, family Closteroviridae) is part of a complex of whitefly-transmitted viruses that cause yellowing disease in cucurbits. Determination of the complete CP, and partial RdRp and Hsp70 sequences of an Algerian CCYV isolate was conducted to unveil the evolutionary relationships with the published isolates in databases. The phylogenetic analysis showed that the Algerian isolate clustered into group I together with the majority of the reported CCYV isolates. The genetic variation within this group was very low (nucleotide identity higher than 99.5%) despite their extensive and discontinuous geographical distribution, plant species (cultivated or weeds) and year of collection. This low genetic divergence could be due to the recentness of this virus that did not face major changes for the present time. In our case, the presence of CCYV was only detected in mixed infection with tomato leaf curl New Delhi virus (ToLCNDV). In future works, it will be interesting to check the likely synergistic or antagonistic interaction between these two viruses that may affect the symptoms development and spread between crops. SUMMARY viiiToLCNDV (genus Begomovirus, family Geminiviridae) was also first detected infecting cucurbit plants in Algeria. ToLCNDV is a bipartite begomovirus that causes destructive epidemics on economically valuable cucurbit and solanaceous crops. The complete DNA-A and DNA-B genomic sequences of a ToLCNDV isolate from cucumber (ToLCNDV-Biskra) were obtained.Alignments of the nucleotide sequences of DNA-A and DNA-B revealed identities of 98.7 and 97.6%, respectively, ...

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A. tumefaciens-mediated transient expression as a tool for antigen production for cucurbit yellow stunting disorder virus

Cited by 8 publications

References 21 publications

High incidence of citrus tristeza virus in mandarin (Citrus reticulata) in North-East states of India

High incidence of citrus tristeza virus in mandarin (Citrus reticulata) in North-East states of India

Whitefly-transmitted criniviruses of cucurbits: current status and future prospects

First report of Cucurbit Chlorotic Yellows Virus (CCYV) and Tomato Leaf Curl New Delhi Virus (ToLCNDV) in Algeria and lack of evidence for seed transmission of ToLCNDV in melon and pumpkin.

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