A Turbidity Test Based Centrifugal Microfluidics Diagnostic System for Simultaneous Detection of HBV, HCV, and CMV

Chang, Hung-Cheng; Chao, Yuan-Hung; Yen, Jia‐Yush; Yu, Yi‐Hsien; Lee, Chun-Nan; Ho, Bing-Ching; Liu, Kou−Chen; Fang, Jiunn; Lin, Chii‐Wann; Lee, Jiun-Haw

doi:10.1155/2015/306708

Cited by 6 publications

(6 citation statements)

References 26 publications

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“…All digital PCR experiments followed the MIQE guidelines for dPCR. 60 The mix used for lyophilization included 2.5 μL of PCR mix, 150 nM primers and probe mix, and 5% trehalose. The sample solution consisted of RNase-free H 2 O, template DNA, and 1.5 μL of 0.2% Tween-20.…”

Section: Methodsmentioning

confidence: 99%

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A Self-Priming Digital Polymerase Chain Reaction Chip for Multiplex Genetic Analysis

et al. 2020

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Digital PCR (polymerase chain reaction) is a powerful and attractive tool for the quantification of nucleic acids. However, the multiplex detection capabilities of this system are limited or require expensive instrumentation and reagents, all of which can hinder multiplex detection goals. Here, we propose strategies toward solving these issues regarding digital PCR. We designed and tested a self-priming digital PCR chip containing 6plex detection capabilities using monochrome fluorescence, which has six detection areas and four-layer structures. This strategy achieved multiplex digital detection by the use of self-priming to preintroduce the specific reaction mix to a certain detection area. This avoids competition when multiple primer pairs coexist, allowing for multiplexing in a shorter time while using less reagents and low-cost instruments. This also prevents the digital PCR chip from experiencing long sample introduction time and evaporation. For further validation, this multiplex digital PCR chip was used to detect five types of EGFR (epidermal growth factor receptor) gene mutations in 15 blood samples from lung cancer patients. We conclude that this technique can precisely quantify EGFR mutations in high-performance diagnostics. This multiplex digital detection chip is a simple and inexpensive test intended for liquid biopsies. It can be applied and used in prenatal diagnostics, the monitoring of residual disease, rapid pathogen detection, and many other procedures.

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Section: Methodsmentioning

confidence: 99%

“…All digital PCR experiments followed the MIQE guidelines for dPCR . The mix used for lyophilization included 2.5 μL of PCR mix, 150 nM primers and probe mix, and 5% trehalose.…”

Section: Methodsmentioning

confidence: 99%

A Self-Priming Digital Polymerase Chain Reaction Chip for Multiplex Genetic Analysis

et al. 2020

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“…HBV is highly contagious and spreads through bodily fluids such as blood, saliva and semen. HBV infection can cause cirrhosis, liver failure or hepatocellular carcinoma (Estevez et al, 2017;Lavanchy, 2004;Sarrazin, 2016).Investigators also use microfluidics to perform convenient and accurate diagnoses for these viruses (Chang et al, 2015;Vaghi et al, 2016). Li et al (Li et al, 2019b) described a disc chip to detect HBV in whole blood.…”

Section: Other Representative Virusesmentioning

confidence: 99%

Advanced “lab-on-a-chip” to detect viruses – Current challenges and future perspectives

Zhuang

Yin

et al. 2020

Biosensors and Bioelectronics

131

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“…On-CD amplification is completed within 60 min with a detection limit of 380 copies. Chang et al [ 231 ] developed a similar multiplex LAMP system for the detection of hepatitis B (HBV), hepatitis C (HCV), and cytomegalo virus (CMV) utilizing Coriolis force for flow control. Oh et al [ 232 ] developed the LAMP-based device shown in Figure 13 for multiplexed detection of four types of foodborne pathogens in milk samples.…”

Section: Novel Microfluidic Actuatorsmentioning

confidence: 99%

Towards Multiplex Molecular Diagnosis—A Review of Microfluidic Genomics Technologies

Basha

Yousuff

et al. 2017

Micromachines

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Highly sensitive and specific pathogen diagnosis is essential for correct and timely treatment of infectious diseases, especially virulent strains, in people. Point-of-care pathogen diagnosis can be a tremendous help in managing disease outbreaks as well as in routine healthcare settings. Infectious pathogens can be identified with high specificity using molecular methods. A plethora of microfluidic innovations in recent years have now made it increasingly feasible to develop portable, robust, accurate, and sensitive genomic diagnostic devices for deployment at the point of care. However, improving processing time, multiplexed detection, sensitivity and limit of detection, specificity, and ease of deployment in resource-limited settings are ongoing challenges. This review outlines recent techniques in microfluidic genomic diagnosis and devices with a focus on integrating them into a lab on a chip that will lead towards the development of multiplexed point-of-care devices of high sensitivity and specificity.

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A Turbidity Test Based Centrifugal Microfluidics Diagnostic System for Simultaneous Detection of HBV, HCV, and CMV

Cited by 6 publications

References 26 publications

A Self-Priming Digital Polymerase Chain Reaction Chip for Multiplex Genetic Analysis

A Self-Priming Digital Polymerase Chain Reaction Chip for Multiplex Genetic Analysis

Advanced “lab-on-a-chip” to detect viruses – Current challenges and future perspectives

Towards Multiplex Molecular Diagnosis—A Review of Microfluidic Genomics Technologies

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