A Two-Component System Acquired by Horizontal Gene Transfer Modulates Gene Transfer and Motility via Cyclic Dimeric GMP

Pallegar, Purvikalyan; Canuti, Marta; Langille, Evan; Peña‐Castillo, Lourdes; Lang, Andrew S.

doi:10.1016/j.jmb.2020.07.001

Cited by 9 publications

(8 citation statements)

References 75 publications

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“…Next, the corresponding response regulator mediates the cellular response. Histidine kinase and reaction regulator are the largest gene families in Gram-negative bacteria and cyanobacteria in particular [ 32 , 33 , 34 , 35 ]. AauSR is a sensor of glutamate.…”

Section: Resultsmentioning

confidence: 99%

Transcriptomic Analysis of Drug-Resistance Acinetobacter baumannii under the Stress Condition Caused by Litsea cubeba L. Essential Oil via RNA Sequencing

Yang

Hao

Jiang

et al. 2021

Genes

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Litsea cubeba L. essential oil (LCEO) can affect the growth of drug-resistance bacteria. However, research on stress response of drug-resistant A. baumannii under sub-lethal LCEO concentrations had been limited so far. Therefore, transcriptomic analysis of A. baumannii under 1/2 minimum inhibitory concentration (MIC, 0.54 mg/mL) of LCEO was performed. Results of transcriptomic analysis showed that 320/352 genes were significantly up/down-regulated, respectively, in LCEO-treated A. baumannii. Both up and down-regulated genes were significantly enriched in three GO terms (oxidation-reduction process; oxidoreductase activity; oxidoreductase activity, acting on the CH-CH group of donors), which indicated that the redox state of A. baumannii was significantly affected by LCEO. LCEO may also inhibit aerobic respiration, synthesis of ketone bodies and the metabolism of some amino acids while, meanwhile, promoting fatty acid degradation of A. baumannii according to Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment. The permeability and the stress of cell membrane of A. baumannii were significantly affected by LCEO. After crystal violet dyeing, the biofilm formation of A. baumannii was promoted/inhibited by extremely low/relatively high concentration of LCEO, respectively. LCEO and chloramphenicol have synergistic growth inhibitory effect against A. baumannii according to the Fractional Inhibitory Concentration Index (FICI) value = 0.375. Our results indicate that the growth of A. baumannii was inhibited by LCEO, and give insights into the stress response of A. baumannii under sub-lethal concentrations of LCEO. These results provided evidence that A. baumannii was inhibited by LCEO, and expanded knowledges of stress response of A. baumannii under sub-lethal concentration of LCEO.

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Section: Resultsmentioning

confidence: 99%

Transcriptomic Analysis of Drug-Resistance Acinetobacter baumannii under the Stress Condition Caused by Litsea cubeba L. Essential Oil via RNA Sequencing

Yang

Hao

Jiang

et al. 2021

Genes

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“…Therefore, we suggest that SqrR regulates the levels of enzymes that metabolize c-di-GMP, resulting in changes in the levels of c-di-GMP which, in turn, regulate the levels of RcGTA [ 21 ]. It has been reported that c-di-GMP-mediated gene transfer is regulated by a two-component system composed of a sensor histidine kinase encoded by rcc00621 and a c-di-GMP catabolic enzyme encoded by rcc00620 as a response regulator [ 49 ]. Our findings indicate a new regulatory pathway of gene transfer via the control of c-di-GMP levels by SqrR.…”

Section: Discussionmentioning

confidence: 99%

Persulfide-Responsive Transcription Factor SqrR Regulates Gene Transfer and Biofilm Formation via the Metabolic Modulation of Cyclic di-GMP in Rhodobacter capsulatus

et al. 2022

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Bacterial phage-like particles (gene transfer agents—GTAs) are widely employed as a crucial genetic vector in horizontal gene transfer. GTA-mediated gene transfer is induced in response to various stresses; however, regulatory mechanisms are poorly understood. We found that the persulfide-responsive transcription factor SqrR may repress the expression of several GTA-related genes in the photosynthetic bacterium Rhodobacter capsulatus. Here, we show that the sqrR deletion mutant (ΔsqrR) produces higher amounts of intra- and extracellular GTA and gene transfer activity than the wild type (WT). The transcript levels of GTA-related genes are also increased in ΔsqrR. In spite of the presumption that GTA-related genes are regulated in response to sulfide by SqrR, treatment with sulfide did not alter the transcript levels of these genes in the WT strain. Surprisingly, hydrogen peroxide increased the transcript levels of GTA-related genes in the WT, and this alteration was abolished in the ΔsqrR strain. Moreover, the absence of SqrR changed the intracellular cyclic dimeric GMP (c-di-GMP) levels, and the amount of c-di-GMP was correlated with GTA activity and biofilm formation. These results suggest that SqrR is related to the repression of GTA production and the activation of biofilm formation via control of the intracellular c-di-GMP levels.

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“…Hence, effective production and release of mature RcGTA particles is dependent on an intact phosphorylation cascade from the response regulator CckA to CtrA via ChpT (Farrera-Calderon et al, 2021;Wang et al, 2014;Westbye et al, 2018). High levels of intracellular c-di-GMP stimulate the phosphatase activity of CckA and help to maintain a higher concentration of unphosphorylated CtrA (Farrera-Calderon et al, 2021;Pallegar et al, 2020aPallegar et al, , 2020b. In its unphosphorylated state, CtrA is required for transcription of gafA (Fogg, 2019).…”

Section: Regulation Of the Rcgta Operonsmentioning

confidence: 99%

“…Low c-di-GMP levels stimulate CckA kinase activity, leading to CtrA phosphorylation (Farrera-Calderon et al, 2021;Pallegar et al, 2020aPallegar et al, , 2020b. CtrA-P also increases expression of the Per-Arnt-Sim (PAS) domain protein DivL, which further enhances CckA kinase activity (Fogg, 2019;Westbye et al, 2018).…”

Section: Regulation Of the Rcgta Operonsmentioning

confidence: 99%

“…The GTA genes are often dispersed at multiple genomic locations (Hynes et al, 2016;Motro et al, 2009), and co-ordinated expression initiates from a small subset of the bacterial population (Fogg, 2019;Fogg et al, 2012;Hynes et al, 2012;Que ´batte and Dehio, 2019). The timing and regulation of GTA production are tightly controlled by interlinked host regulatory circuits, including quorum sensing (Koppenho ¨fer et al, 2019;Leung et al, 2012), stringent response (Que ´batte et al, 2017;Westbye et al, 2017), SOS response (Kuchinski et al, 2016), cyclic diguanylate (c-di-GMP) (Pallegar et al, 2020a;2020b), and the pleiotropic transcription factor CtrA (Lang and Beatty, 2000;Westbye et al, 2018). In R. capsulatus, these complex pathways are integrated via a specific GTA transcriptional regulator, GafA (Fogg, 2019), and a repeats in toxin superfamily-domain (RTX) extracellular repressor, rcc00280 (Ding et al, 2019;Westbye et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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A Two-Component System Acquired by Horizontal Gene Transfer Modulates Gene Transfer and Motility via Cyclic Dimeric GMP

Cited by 9 publications

References 75 publications

Transcriptomic Analysis of Drug-Resistance Acinetobacter baumannii under the Stress Condition Caused by Litsea cubeba L. Essential Oil via RNA Sequencing

Transcriptomic Analysis of Drug-Resistance Acinetobacter baumannii under the Stress Condition Caused by Litsea cubeba L. Essential Oil via RNA Sequencing

Persulfide-Responsive Transcription Factor SqrR Regulates Gene Transfer and Biofilm Formation via the Metabolic Modulation of Cyclic di-GMP in Rhodobacter capsulatus

The archetypal gene transfer agent RcGTA is regulated via direct interaction with the enigmatic RNA polymerase omega subunit

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