A two‐photon europium complex as specific endoplasmic reticulum probe

Abstract

Highly emissive europium complexes with specific endoplasmic reticulum localization potential includes several advantages such as fast uptake, long resident lifetime, low dosage requirement, low cytotoxicity and highly emissive two-photon induced f-f emission imaging.

Bionanoprobes with Excellent Two‐Photon‐Sensitized Eu3+ Luminescence Properties for Live Cell Imaging

“…[6,7] The excitation energy transfer from dpbt to Eu 3 + in [EuA C H T U N G T R E N N U N G (tta) 3 dpbt] is dominated by a singlet energy-transfer pathway. [6, 7d] [Eu-A C H T U N G T R E N N U N G (tta) 3 dpbt] nanoparticles protected by cetyltrimethyl ammonium bromide (CTAB) in water/methanol mixtures exhibited excellent visible-light-sensitized and two-photon-sensitized luminescence properties with a d F value of 3. 2 10 5 GM (1 GM = 10 À50 cm 4 s photon À1 particle…”

“…Herein, we report novel nanospheres (EuLNPs), characterized by encapsulation of [EuA C H T U N G T R E N N U N G (tta) 3 A stable colloidal solution of EuLNPs in water was prepared easily by adding an acetone solution containing [Eu-A C H T U N G T R E N N U N G (tta) 3 dpbt] and PMMA-co-MAA to pure water and the treatment processes described in the Experimental Section. Due to the potential applications ranging from controlled release to rheological modifications, the micellization behavior of the block copolymer poly(methyl methacrylate-blockmethacrylic acid) (PMMA-b-MAA) and its core shell-like micelles in aqueous medium have been well studied.…”

“…[2][3][4] Due to the characteristic luminescence properties of Eu 3 + complexes, such as the characteristic narrow-line emission in the red-light wavelength region with acceptable transparence for many biosamples, high luminescence quantum yields, large Stokes shifts, and long luminescence lifetime (millisecond order), the TPS Eu 3 + luminescence is of significance for developing bioanalysis or bioimaging technologies.…”

“…The maximal molar extinction coefficient (e) of [EuA C H T U N G T R E N N U N G (tta) 3 dpbt] molecules in the EuLNPs colloidal solution was estimated to be 6.5 10 4 m À1 cm À1 at 412 nm, which was approximate to that of [EuA C H T U N G T R E N N U N G (tta) 3 ).…”

“…[11] In the calculation of the luminescence quantum yield of prepared nanoparticles, the influence of scattering intensity was not removed, that is, the luminescence quantum yield of [Eu-A C H T U N G T R E N N U N G (tta) 3 dpbt] in EuLNPs is higher than that of the nanoparticles. Assuming that the density of EuLNPs is about 1.1 g cm À3 (the densities of PMMA-co-MAA and [Eu-A C H T U N G T R E N N U N G (tta) 3 dpbt] are 1.16 and 0.92 g cm À3 , respectively), it can be estimated that each nanosphere with the average diameter (d av = 52 nm) contains about 3500 [EuA C H T U N G T R E N N U N G (tta) 3 dpbt] molecules (see the Supporting Information for details).…”