A two-photon metal-organic framework nanoprobe with catalytic hairpin assembly for amplified MicroRNA imaging in living cells and tissues

Yang, Chan; Wang, Kun; Li, Zihong; Mo, Liuting; Lin, Weiying

doi:10.1016/j.snb.2022.131593

Cited by 22 publications

(11 citation statements)

References 45 publications

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“…Dual-mode nanoprobes enter into tissue sections by osmosis. 34 First, the AuNCs@SiO 2 -BHQ2-GO nanoprobe was cultured with rat liver tissue slices for 4.0 h. The nanoprobes were then removed from the tissue surface by repeated cleaning. Finally, Z-scan imaging was performed on rat liver tissue slices by OP and TP microscopy, respectively.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…Fluorescence scanning imaging of rat liver tissue sections was performed to verify whether the dual-mode nanoprobe AuNCs@SiO 2 -BHQ2-GO could achieve fluorescence imaging of deep tissues. Dual-mode nanoprobes enter into tissue sections by osmosis . First, the AuNCs@SiO 2 -BHQ2-GO nanoprobe was cultured with rat liver tissue slices for 4.0 h. The nanoprobes were then removed from the tissue surface by repeated cleaning.…”

Section: Resultsmentioning

confidence: 99%

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Dual-Mode Gold Nanocluster-Based Nanoprobe Platform for Two-Photon Fluorescence Imaging and Fluorescence Lifetime Imaging of Intracellular Endogenous miRNA

Li,

Yu,

Feng

et al. 2023

Anal. Chem.

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Bioimaging is widely used in various fields of modern medicine. Fluorescence imaging has the advantages of high sensitivity, high selectivity, noninvasiveness, in situ imaging, and so on. However, one-photon (OP) fluorescence imaging has problems, such as low tissue penetration depth and low spatiotemporal resolution. These disadvantages can be solved by two-photon (TP) fluorescence imaging. However, TP imaging still uses fluorescence intensity as a signal. The complexity of organisms will inevitably affect the change of fluorescence intensity, cause false-positive signals, and affect the accuracy of the results obtained. Fluorescence lifetime imaging (FLIM) is different from other kinds of fluorescence imaging, which is an intrinsic property of the material and independent of the material concentration and fluorescence intensity. FLIM can effectively avoid the fluctuation of TP imaging based on fluorescence intensity and the interference of autofluorescence. Therefore, based on silica-coated gold nanoclusters (AuNCs@SiO 2 ) combined with nucleic acid probes, the dual-mode nanoprobe platform was constructed for TP and FLIM imaging of intracellular endogenous miRNA-21 for the first time. First, the dual-mode nanoprobe used a dual fluorescence quencher of BHQ2 and graphene oxide (GO), which has a high signal-to-noise ratio and anti-interference. Second, the dual-mode nanoprobe can detect miR-21 with high sensitivity and selectivity in vitro, with a detection limit of 0.91 nM. Finally, the dual-mode nanoprobes performed satisfactory TP fluorescence imaging (330.0 μm penetration depth) and FLIM (τ ave = 50.0 ns) of endogenous miR-21 in living cells and tissues. The dual-mode platforms have promising applications in miRNA-based early detection and therapy and hold much promise for improving clinical efficacy.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Dual-Mode Gold Nanocluster-Based Nanoprobe Platform for Two-Photon Fluorescence Imaging and Fluorescence Lifetime Imaging of Intracellular Endogenous miRNA

Li,

Yu,

Feng

et al. 2023

Anal. Chem.

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“…Yang et al reported a novel two-photon nanoprobe TP-CHA-UiO-66, 105 using MOF as a nanocarrier and hairpins as a two-photon fluorescent signal amplifier for miRNA imaging of tumour cells and deep tissues. Where TP was a fluorophore, and its excellent imaging performance could facilitate and boost imaging of tumour-associated miRNAs in live tumour cells and tissues.…”

Section: Application Of Mofs In the Detection Of Organic Moleculesmentioning

confidence: 99%

Metal–organic frameworks (MOFs) as apt luminescent probes for the detection of biochemical analytes

et al. 2023

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“…The authors have cited additional references within the Supporting Information. [25][26][27][28][29]…”

Section: Supporting Informationmentioning

confidence: 99%

An Intelligent Laser‐Free Photodynamic Therapy Based on Endogenous miRNA‐Amplified CRET Nanoplatform**

Weng

Wang

et al. 2023

Chemistry A European J

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Laser-free photodynamic therapy (PDT) is a promising noninvasive therapeutic modality for deep-seated tumor, yet is constrained by low efficiency due to the limited stimulation strategies. Herein, a novel miRNA-responsive laser-free PDT was developed through metal-organic frameworks (MOFs)-mediated chemiluminescence resonance energy transfer (CRET) nanoplatform. The photosensitizer chlorin e6 (Ce6)-loaded MOFs were functionalized with hairpin nucleic acids for sensitive responsiveness of tumor biomarker miRNA through catalytic hairpin assembly (CHA), which enabled the amplified assembly of horseradish peroxidase (HRP)-mimicking hemin/G-quadruplex DNAzyme on MOFs. Simultaneously, the on-MOF assembled DNAzymes efficiently catalyzed chemiluminescence reaction to stimulate adjacent Ce6 in the presence of luminol and H 2 O 2 , thus allowing the CRET-mediated Ce6 luminescence and reactive oxygen species (ROS) generation for self-illuminating PDT. The CRET nanoplatform achieved significant malignant cell apoptosis and tumor inhibition effects without external laser irradiation. It is envisioned that the miRNA-amplified CRET nanoplatform might be a selective and highly efficient antitumor nanomedicine for precise theranostic.

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A two-photon metal-organic framework nanoprobe with catalytic hairpin assembly for amplified MicroRNA imaging in living cells and tissues

Cited by 22 publications

References 45 publications

Dual-Mode Gold Nanocluster-Based Nanoprobe Platform for Two-Photon Fluorescence Imaging and Fluorescence Lifetime Imaging of Intracellular Endogenous miRNA

Dual-Mode Gold Nanocluster-Based Nanoprobe Platform for Two-Photon Fluorescence Imaging and Fluorescence Lifetime Imaging of Intracellular Endogenous miRNA

Metal–organic frameworks (MOFs) as apt luminescent probes for the detection of biochemical analytes

An Intelligent Laser‐Free Photodynamic Therapy Based on Endogenous miRNA‐Amplified CRET Nanoplatform**

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