A two-step morphology-PCR strategy for the identification of nematode larvae recovered from muscles after artificial digestion at meat inspection

Karadjian, Grégory; Kaestner, Carolyn; Laboutière, Lisa; Adicéam, Emilie; Wagner, Thomas; Johne, Annette; Thomas, Myriam; Polack, Bruno; Mayer‐Scholl, Anne; Vallée, Isabelle

doi:10.1007/s00436-020-06899-7

Cited by 11 publications

(16 citation statements)

References 39 publications

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“…Further, the 18S PCR protocol published by Karadjian et al [ 3 ] for the identification of nematodes was applied to 20 randomly selected AM samples, followed by a sequence analysis to determine the suitability of the PCR for the detection and identification of A. alata .…”

Section: Methodsmentioning

confidence: 99%

“…In addition, the three samples containing Opisthioglyphe ranae larvae were examined using this 18S PCR [ 3 ], followed by a sequence analysis, giving a total of 23 larvae-containing samples that were tested by this PCR.…”

Section: Methodsmentioning

confidence: 99%

“…Both the Alaria spp.-specific PCR and the 18S PCR were carried out using the protocols published by Riehn et al [ 22 ] and Karadjian et al [ 3 ], respectively.…”

Section: Methodsmentioning

confidence: 99%

“…All PCR products generated by the 18S PCR [ 3 ] were sent to Eurofins Genomics (Ebersberg, Germany) for sequence analysis.…”

Section: Methodsmentioning

confidence: 99%

“…2020/1478 [ 2 ]. During the inspection of game meat, wide varieties of parasites, which do not belong to the Trichinella genus, are frequently detected [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

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Development of a Novel Method for Identification of Alaria alata Mesocercariae by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

et al. 2021

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Alaria (A.) alata mesocercariae (AM) have increasingly appeared as incidental findings during the mandatory inspection of wild boars for Trichinella in many European countries. An Alaria spp.-specific PCR is available for the identification of AM; however, it is time- and cost-intensive. Therefore, we propose a rapid and cost-efficient MALDI-TOF assay for the identification of AM in wild boar meat that can be applied in routine diagnostics. In this study, a fast and methodologically simple protocol for the protein extraction of AM from different host species in different countries was established, and an AM-specific reference spectra database was created as part of the ongoing development of an existing Trichinella spp. database. A formic acid protein extraction was performed after pooling 10 AM from the same host individual. In total, 61 main spectra profiles (MSPs) from different host individuals were stored in an AM-specific MSP library. The cluster analysis of these 61 MSPs indicated a possible variation within the A. alata species with a tentative association with the geographical origin of the host, but not the host species. This MALDI-TOF assay allows for a fast verification of the AM isolates, which is the next step in the development of a universal database for the identification of several parasites isolated from meat.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…Both the Alaria spp.-specific PCR and the 18S PCR were carried out using the protocols published by Riehn et al [ 22 ] and Karadjian et al [ 3 ], respectively.…”

Section: Methodsmentioning

confidence: 99%

“…All PCR products generated by the 18S PCR [ 3 ] were sent to Eurofins Genomics (Ebersberg, Germany) for sequence analysis.…”

Section: Methodsmentioning

confidence: 99%

“…2020/1478 [ 2 ]. During the inspection of game meat, wide varieties of parasites, which do not belong to the Trichinella genus, are frequently detected [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations