A Type IIa crustin from the pink shrimp Farfantepenaeus paulensis (crusFpau) is constitutively synthesized and stored by specific granule-containing hemocyte subpopulations

Bandeira, Paula Terra; Vernal, Javier; Matos, Gabriel Machado; Farias, Natanael Dantas; Terenzi, Hernán; Pinto, Aguinaldo Roberto; Barracco, Margherita Anna; Rosa, Rafael Diego

doi:10.1016/j.fsi.2019.12.055

Cited by 20 publications

(20 citation statements)

References 40 publications

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“…Thus, there may be a phagocytic subpopulation that becomes functional soon after entering the circulation. The heterogeneity of cells in the same morphological group is supported by previous findings showing their difference in staining properties 24 – 26 , gene expression patterns 27 , 28 , or immune function 23 , 29 .…”

Section: Discussionsupporting

confidence: 79%

“…HCs are thought to be immature hemocytes occasionally released from HPT under certain conditions 14 . The existence of subpopulations of the morphological types of hemocytes has been proposed in decapods based on their staining property 24 – 26 , gene expression pattern 27 , 28 , or immune function 23 , 29 , but with no in-depth study.…”

Section: Introductionmentioning

confidence: 99%

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Crayfish hemocytes develop along the granular cell lineage

Zheng

et al. 2021

Sci Rep

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Despite the central role of hemocytes in crustacean immunity, the process of hemocyte differentiation and maturation remains unclear. In some decapods, it has been proposed that the two main types of hemocytes, granular cells (GCs) and semigranular cells (SGCs), differentiate along separate lineages. However, our current findings challenge this model. By tracking newly produced hemocytes and transplanted cells, we demonstrate that almost all the circulating hemocytes of crayfish belong to the GC lineage. SGCs and GCs may represent hemocytes of different developmental stages rather than two types of fully differentiated cells. Hemocyte precursors produced by progenitor cells differentiate in the hematopoietic tissue (HPT) for 3 ~ 4 days. Immature hemocytes are released from HPT in the form of SGCs and take 1 ~ 3 months to mature in the circulation. GCs represent the terminal stage of development. They can survive for as long as 2 months. The changes in the expression pattern of marker genes during GC differentiation support our conclusions. Further analysis of hemocyte phagocytosis indicates the existence of functionally different subpopulations. These findings may reshape our understanding of crustacean hematopoiesis and may lead to reconsideration of the roles and relationship of circulating hemocytes.

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Section: Discussionsupporting

confidence: 79%

Section: Introductionmentioning

confidence: 99%

Crayfish hemocytes develop along the granular cell lineage

Zheng

et al. 2021

Sci Rep

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“…The antimicrobial properties of crustins have been reported by many research groups [32,33]. Generally, crustins exhibit a broad-spectrum of antibacterial activities against Gram-positive and Gram-negative bacteria and fungi [34].…”

Section: Discussionmentioning

confidence: 99%

A Crustin from Hydrothermal Vent Shrimp: Antimicrobial Activity and Mechanism

et al. 2021

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Crustin is a type of antimicrobial peptide and plays an important role in the innate immunity of arthropods. We report here the identification and characterization of a crustin (named Crus1) from the shrimp Rimicaris sp. inhabiting the deep-sea hydrothermal vent in Manus Basin (Papua New Guinea). Crus1 shares the highest identity (51.76%) with a Type I crustin of Penaeus vannamei and possesses a whey acidic protein (WAP) domain, which contains eight cysteine residues that form the conserved ‘four-disulfide core’ structure. Recombinant Crus1 (rCrus1) bound to peptidoglycan and lipoteichoic acid, and effectively killed Gram-positive bacteria in a manner that was dependent on pH, temperature, and disulfide linkage. rCrus1 induced membrane leakage and structure damage in the target bacteria, but had no effect on bacterial protoplasts. Serine substitution of each of the 8 Cys residues in the WAP domain did not affect the bacterial binding capacity but completely abolished the bactericidal activity of rCrus1. These results provide new insights into the characteristic and mechanism of the antimicrobial activity of deep sea crustins.

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“…Crustins are commonly reported to have antimicrobial activity against gram-positive bacteria, gram-negative bacteria, fungi and even viruses in vitro, such as Lv-SWD4 (15), SpCrus4 (16), SpCrus5 (17), SpCrus6 (8), crusFpau (18), EsDWD (19), PtCrustin2 (20), and CrustinPm7 (21). They also have good resistance to important clinical pathogens such as Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Candida albicans, and several common aquatic pathogens including Aeromonas hydrophila, Vibrio parahaemolyticus and Vibrio anguillarum.…”

Section: Introductionmentioning

confidence: 99%

A New Crustin Gene Homolog SpCrus8 Identified in Scylla paramamosain Exerting In Vivo Protection Through Opsonization and Immunomodulation

Jiang

Chen

et al. 2022

Front. Immunol.

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Crustins are the most abundant class of antimicrobial peptides in crustaceans and are essential for protecting animals from infection. Among them, type II crustins usually exhibit potent antimicrobial activity. Interestingly, in this study, a newly identified type II crustin gene homolog (named SpCrus8) from mud crab Scylla paramamosain, the recombinant proteins of which (rSpCrus8 and rTrx-SpCrus8) showed no obvious antibacterial effects, but could significantly reduce the bacterial load in crab hemolymph and improve the survival rate of crabs infected with Vibrio alginolyticus. The immune-related function of SpCrus8 and the underlying mechanism deserve further investigation. It was found that the SpCrus8 gene was widely distributed in various tissues of adult crabs. In the hepatopancreas of crabs infected with V. alginolyticus or Staphylococcus aureus, transcripts of the SpCrus8 gene were remarkably induced, indicating that the SpCrus8 gene was involved in the immune response to bacterial infection in vivo. In addition, rSpCrus8 and rTrx-SpCrus8 had strong binding activity not only to microbial surface components (lipopolysaccharide, lipoteichoic acid, peptidoglycan, and glucan), but also to the tested bacteria (S. aureus, Pseudomonas aeruginosa and V. alginolyticus). Notably, rSpCrus8 and rTrx-SpCrus8 could significantly promote hemocyte phagocytosis. After rSpCrus8 and rTrx-SpCrus8 treatment, a large number of fluorescent microspheres were observed to aggregate into clusters and be phagocytosed by multiple hemocytes, while hemocytes in the control group phagocytosed only individual microspheres, indicating that SpCrus8 played an important role in opsonization. When the SpCrus8 gene was knocked down, the expression levels of the key phagocytosis-related genes SpRab5 and SpRab7 were significantly downregulated, as well as the IMD signaling pathway genes SpIKKβ and SpRelish, and another crustin gene SpCrus5. Correspondingly, all the SpIKKβ, SpRelish and SpCrus5 genes were significantly upregulated after rSpCrus8 treatment, suggesting that SpCrus8 might be involved in the immunomodulation of S. paramamosain. Taken together, this study revealed the immune-related functions of the SpCrus8 gene in opsonization and regulation, which will help us further understand the role of the crustin gene family in the immune system of mud crabs and provide new insights into the function of type II crutins.

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A Type IIa crustin from the pink shrimp Farfantepenaeus paulensis (crusFpau) is constitutively synthesized and stored by specific granule-containing hemocyte subpopulations

Cited by 20 publications

References 40 publications

Crayfish hemocytes develop along the granular cell lineage

Crayfish hemocytes develop along the granular cell lineage

A Crustin from Hydrothermal Vent Shrimp: Antimicrobial Activity and Mechanism

A New Crustin Gene Homolog SpCrus8 Identified in Scylla paramamosain Exerting In Vivo Protection Through Opsonization and Immunomodulation

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