A tyrosinase fluorescent probe with large Stokes shift and high fluorescence enhancement for effective identification of liver cancer cells

Sun, Qi; Guo, Yun; Li, Xiang; Luo, Xiaogang; Qiu, Yuan; Liu, Genyan

doi:10.1016/j.saa.2022.121831

Cited by 9 publications

(3 citation statements)

References 42 publications

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“…With the addition of LFP, the fluorescence peak of TYR was redshifted, indicating a decrease in the energy required for TYR to maintain its own structure and a change in its conformation (Chen et al, 2022). The results suggested that compounds in LFP especially flavonoids may combine with TYR to quench its intrinsic fluorescence and affect the microenvironment hydrophobicity of fluorescent residues in tyrosinase (Sun et al, 2023). The combination could be due to the hydrophobic interaction between flavonoids in LFP and amino acid residues in tyrosinase, resulting in the exposure of protein polypeptide chains to the environment, thereby inhibiting the catalytic reaction of tyrosinase (Lai et al, 2023).…”

Section: Effect Of Lfp On Conformation Of Tyrosinasementioning

confidence: 96%

Inhibitive Mechanism of Loquat Flower Isolate on Tyrosinase Activity and Melanin Synthesis in Mouse Melanoma B16 Cells

Chen,

Tao,

Wang

et al. 2024

Preprint

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Melanin is extensively distributed in organisms and believed to be synthetized by tyrosinase (TYR), leading to aberrant coloring and skin conditions when over-produced. Loquat (Eriobotrya japonica (Thunb.) Lindl.) flowers contain large amounts of bioactive substances currently few studied on their suppressive capabilities to melanin synthesis. Its isolate product (LFP) was obtained by ethanol solution extraction and resin purification, of which the inhibitory mechanism on TYR activity was elaborated by enzyme kinetics and multi-spectroscopy determinations and the impact on related proteins’ expression in mouse melanoma B16 cells was analyzed with Western blotting. HPLC-MS analysis indicated that LFP was identified into 137 compounds, with 12 including flavonoids (quercetin, isorhamnoin, p-coumaric acid, etc.), cinnamic acid and its derivatives as well as benzene and its derivatives owning TYR inhibitory activities. LFP inhibited TYR activity in a concentration-dependent manner with its IC50 value being 2.8 mg/mL and an anti-competitive mechanism involved altering the enzyme's conformation. LFP reduced the expression of TYR, TRP1, and TRP2 in melanoma B16 cells, hence inhibiting the synthesis of melanin. Considered collectively, LFP would be a viable option for treating hyperpigmentation caused by tyrosinase, providing a theoretical basis for the value-added utilization of loquat flower resource in beauty and aging related functional products.

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Section: Effect Of Lfp On Conformation Of Tyrosinasementioning

confidence: 96%

Inhibitive Mechanism of Loquat Flower Isolate on Tyrosinase Activity and Melanin Synthesis in Mouse Melanoma B16 Cells

Chen,

Tao,

Wang

et al. 2024

Preprint

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“…Thus HFC-TYR had the potential to become a specific probe for liver cancer diagnosis. 78 In 2022, Duan et al utilized the reaction of ExPh2-CN and acetyl chloride to synthesize the probe ExPh2-CNAc2 for the detection of CE (Fig. 31G).…”

Section: Enzyme-targeting Probesmentioning

confidence: 99%

mentioning

confidence: 99%

Activatable fluorescent probes for early diagnosis and evaluation of liver injury

Lin,

Jiang,

Liu

et al. 2024

Analyst

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A turn-on fluorescence probe for imaging tyrosinase at the wound site in broken tail of zebrafish

Chen,

Ji,

Sun

et al. 2024

Bioorganic Chemistry

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A tyrosinase fluorescent probe with large Stokes shift and high fluorescence enhancement for effective identification of liver cancer cells

Cited by 9 publications

References 42 publications

Inhibitive Mechanism of Loquat Flower Isolate on Tyrosinase Activity and Melanin Synthesis in Mouse Melanoma B16 Cells

Inhibitive Mechanism of Loquat Flower Isolate on Tyrosinase Activity and Melanin Synthesis in Mouse Melanoma B16 Cells

Activatable fluorescent probes for early diagnosis and evaluation of liver injury

A turn-on fluorescence probe for imaging tyrosinase at the wound site in broken tail of zebrafish

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