2020
DOI: 10.5194/egusphere-egu2020-17791
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A unified protocol for the high-throughput measurement of PLFA, NLFA, GLFA and sterols from soil

Abstract: <p>Phospholipid fatty acids (PLFA) are widely used as biomarkers for soil microbial biomass. In more recent years, neutral lipid fatty acids (NLFA) have additionally been used as storage biomarkers. Both lipid classes are usually separated via silica solid phase extraction (SPE) after extraction with a mixture of chloroform, methanol and citric acid buffer. However, in recent years several studies reported incomplete or inconsistent separation of lipid classes, depending on minor differences in t… Show more

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“…Ergosterol and PLFA could be both considered as bioindicators of fungal biomass but since they corresponded to different lipid classes, they could be affected variously by agricultural practices [63]. Moreover, the 18S rDNA amplification was also used to address the abundance of fungi in soils.…”
Section: Ergosterol Analysismentioning
confidence: 99%
“…Ergosterol and PLFA could be both considered as bioindicators of fungal biomass but since they corresponded to different lipid classes, they could be affected variously by agricultural practices [63]. Moreover, the 18S rDNA amplification was also used to address the abundance of fungi in soils.…”
Section: Ergosterol Analysismentioning
confidence: 99%