A unique bacterial secretion machinery with multiple secretion centers

Song, Liqiang; Perpich, John D.; Wu, Chenggang; Doan, Thierry; Nowakowska, Zuzanna; Potempa, Jan; Christie, Peter J.; Cascales, Éric; Lamont, Richard J.; Hu, Bo

doi:10.1073/pnas.2119907119

Cited by 24 publications

(27 citation statements)

References 48 publications

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“…GldJ lipoprotein shares 30% identity with GldK lipoprotein over 382 amino acids 33 . P. gingivalis PorK (GldK homolog) with PorN makes a ring structure of ~8 nm in thickness and 52 nm in diameter 14 , 40 . The thickness of fibers in the multirail structure involving GldJ is almost the same as that of the PorK/PorN ring.…”

Section: Discussionmentioning

confidence: 99%

“…T9SSs were first identified and studied in the nonmotile oral pathogen Porphyromonas gingivalis and in F. johnsoniae [8][9][10][11] . Recent studies have revealed that T9SS is a unique system that is clearly different from other secretion systems in terms of its supramolecular structure and secretion mechanism [12][13][14] . F. johnsoniae cells use the T9SS to secrete dozens of proteins, including soluble extracellular enzymes and motility adhesins that reside on the cell surface [15][16][17] .…”

mentioning

confidence: 99%

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Filamentous structures in the cell envelope are associated with bacteroidetes gliding machinery

et al. 2023

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Many bacteria belonging to the phylum Bacteroidetes move on solid surfaces, called gliding motility. In our previous study with the Bacteroidetes gliding bacterium Flavobacterium johnsoniae, we proposed a helical loop track model, where adhesive SprB filaments are propelled along a helical loop on the cell surface. In this study, we observed the gliding cell rotating counterclockwise about its axis when viewed from the rear to the advancing direction of the cell and revealed that one labeled SprB focus sometimes overtook and passed another SprB focus that was moving in the same direction. Several electron microscopic analyses revealed the presence of a possible multi-rail structure underneath the outer membrane, which was associated with SprB filaments and contained GldJ protein. These results provide insights into the mechanism of Bacteroidetes gliding motility, in which the SprB filaments are propelled along tracks that may form a multi-rail system underneath the outer membrane. The insights may give clues as to how the SprB filaments get their driving force.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Filamentous structures in the cell envelope are associated with bacteroidetes gliding machinery

et al. 2023

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“…GldJ lipoprotein shares 30% identity with GldK lipoprotein over 382 amino acids 33 . P. gingivalis PorK (GldK homolog) with PorN makes a ring structure of ~ 8 nm in thickness and 52 nm in diameter 14,40 . It is worthy of note that the thickness of ber in the multi-rail structure involving GldJ is almost the same as that of the PorK/PorN ring.…”

Section: Discussionmentioning

confidence: 99%

“…T9SSs were rst identi ed and studied in the nonmotile oral pathogen Porphyromonas gingivalis and in F. johnsoniae [8][9][10][11] . Recent studies have revealed that T9SS is a unique system that is clearly different from other secretion systems in terms of its supramolecular structure and secretion mechanism [12][13][14] . F. johnsoniae cells use the T9SS to secrete dozens of proteins including soluble extracellular enzymes and motility adhesins that reside on the cell surface [15][16][17] .…”

Section: Introductionmentioning

confidence: 99%

A Multi-Rail Structure in the Cell Envelope for the Bacteroidetes Gliding Machinery

Shibata¹,

Tahara²,

Katayama³

et al. 2022

Preprint

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Many bacteria belonging to the phylum Bacteroidetes move on solid surfaces, which is called gliding motility. In our previous study with the Bacteroidetes gliding bacterium Flavobacterium johnsoniae, we proposed a helical loop track model, where adhesive SprB filaments are propelled along a left-handed closed helical loop on the cell surface. Attachment of SprB to the substratum results in cell movement. In this study, we observed the gliding cell rotating counterclockwise about its axis when viewed from the rear to the advancing direction of the cell, which was consistent with the helical loop track model. Total internal reflection fluorescence microscopy of SprB on a gliding cell revealed that one labeled SprB focus sometimes overtook and passed another SprB focus that was moving in the same direction, suggesting the presence of multiple lanes in the helical loop track. Several electron microscopic analyses revealed the presence of a multi-rail structure underneath the outer membrane, which was associated with SprB filaments and contained GldJ protein. A similar structure was observed in the distantly related marine gliding Bacteroidetes Saprospira grandis. These results provide new insights into the mechanism of Bacteroidetes gliding motility, in which the SprB filaments are propelled along the multi-rails underneath the outer membrane.

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“…PorK/GldK is an OM lipoprotein whereas PorN/GldN locates in the periplasm [13,21]. In Porphyromonas, the PorK and PorN proteins interact and assemble a 36-mer, 50 nm large ring [20,21]. This ring is located underneath the OM and is connected to 18 PorLM complexes via interactions between PorN and the PorM D4 domains, building an overall birdcage-like structure in the periplasm [13,16,20,25].…”

Section: The Om-associated Complexmentioning

confidence: 99%

A journey with type IX secretion system effectors: selection, transport, processing and activities

et al. 2023

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The type IX secretion system (T9SS) is a multiprotein machine distributed in Bacteroidota and responsible for the secretion of various proteins across the outer membrane. Secreted effectors can be either delivered into the medium or anchored to the cell surface. The T9SS is composed of a transenvelope complex consisting of proton-motive force-dependent motors connected to a membrane-associated ring and outer membrane translocons, and a cell-surface anchoring complex that processes effectors once translocated. The T9SS is involved in pathogenesis, metal acquisition, carbohydrate degradation, S-layer biogenesis and gliding motility. The broad spectrum of functions is linked to a highly versatile repertoire of effectors including metallophores, enzymes, toxins and adhesins, that all possess specific signatures to be recruited and transported by the apparatus. This review summarizes the current knowledge on T9SS substrate secretion signals, transport, processing and activities.

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A unique bacterial secretion machinery with multiple secretion centers

Cited by 24 publications

References 48 publications

Filamentous structures in the cell envelope are associated with bacteroidetes gliding machinery

Filamentous structures in the cell envelope are associated with bacteroidetes gliding machinery

A Multi-Rail Structure in the Cell Envelope for the Bacteroidetes Gliding Machinery

A journey with type IX secretion system effectors: selection, transport, processing and activities

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