A unique kinesin‐like protein, Klp8, is involved in mitosis and cell morphology through microtubule stabilization

Abstract

Kinesins are microtubule (MT)‐based motors involved in various cellular functions including intracellular transport of vesicles and organelles, and dynamics of chromosomes during cell division. The fission yeast Schizosaccharomyces pombe expresses nine kinesin‐like proteins (klps). Klp8 is one of them and has not been characterized yet though it has been reported to localize at the division site. Here, we studied function and localization of Klp8 in S. pombe cells. The gene klp8+ was not essential for both viability and cytoskeletal organization. Klp8‐YFP was concentrated as medial cortical dots during interphase, and organized into a ring at the division site during mitosis. The Klp8 ring seemed to be localized in the space between the actomyosin contractile ring and the plasma membrane. The Klp8 ring shrank as cytokinesis proceeded. In klp8‐deleted (Δ) cells, the speed of spindle elongation during anaphase B was slowed down. Overproduction of Klp8 caused bent or elongated cells, in which MTs were abnormally elongated and less dynamic than those in normal cells. Deletion of klp8+ gene suppressed the delay in mitotic entry in blt1Δ cells. These results suggest that Klp8 is involved in mitosis and cell morphology through MT stabilization.

Kinesin family member 2A high expression correlates with advanced tumor stages and worse prognosis in non‐small cell lung cancer patients

“…The kinesin proteins are microtubules depolymerases, which are involved in mitosis, cell migration, trafficking, and cell signaling . KIF2A, as one member of the kinesin protein family, has attracted a lot of attention due to its implication in the development and progression of several tumors .…”

Kinesin Motors in the Filamentous Basidiomycetes in Light of the Schizophyllum commune Genome

“…In the budding yeast Saccharomyces cerevisiae Meyen ex E.C. Hansen five [ 16 ], in the fission yeast Schizosaccaromyces pombe Lindner nine [ 17 , 18 ], in the filamentous ascomycetes Aspergillus nidulans (Eidam) Winter and Neurospora crassa Shear et Dodge [ 18 , 19 ], and in the basidiomycete smut U. maydis [ 20 ], nine to eleven kinesin motor proteins have been identified, respectively, but an extensive survey of the kinesins found in filamentous basidiomycetes (Agaricomycetes) is missing.…”

“…In the budding yeast, the five kinesin genes belong to four kinesin families [ 16 ]. S. pombe kinesin-8 and -14 families [ 17 ], and in A. nidulans kinesin-3 family, have each two members [ 19 ].…”

Regulación de la citoquinesis por Rgf1 y la ruta de integridad

“…Durante interfase una fracción de Mid1, quien también se une a membranas y oligomeriza, interacciona con Cdr2 y juntos promueven el reclutamiento de otros componentes a los nodos. Aquí se incluyen las kinasas de ciclo Wee1 y su inhibidor Cdr1, que contribuyen al control del tamaño celular (Moseley et al 2009), y otros nodos interfásicos que permanecen tras la separación celular (Akamatsu et al 2014) formados por la proteína de unión a membrana Blt1 (Guzman-Vendrell et al 2013), la kinesina Klp8 (Kashiwazaki et al 2019), el Rho-GEF Gef2 (Ye et al 2012) y la proteína asociada Nod1 (Jourdain et al 2013;Zhu et al 2013).…”