A Unique VP4 Gene Allele Carried by an Unusual Bovine Rotavirus Strain, 993/83

Isegawa, Yuji; Nakagomi, Osamu; Brüssow, Harald; Minamoto, Nobuyuki; Nakagomi, Toyoko; Ueda, Shigeo

doi:10.1006/viro.1994.1043

Cited by 22 publications

(9 citation statements)

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“…Strains belonging to serotypes G6, G8 and G10 are the major pathogens in cattle, though rare occurrence of strains belonging to G1, G2, G7 and G11 has been reported [6,31,34]. The predominant bovine serotypes G6, G8 and G10 possess either of the three VP4 types P6 [1], P7 [5] and P8 [11] [19,53].…”

Section: Introductionmentioning

confidence: 99%

Prevalence of, and antigenic variation in, serotype G10 rotaviruses and detection of serotype G3 strains in diarrheic calves: Implications for the origin of G10P11 or P11 type reassortant asymptomatic strains in newborn children in India

Varshney

Jagannath

Vethanayagam

et al. 2002

Archives of Virology

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Previous studies have shown predominant association of G10P11 type bovine rotavirus-derived reassortant strains with asymptomatic infections in newborn children in India. To understand the epidemiological and genetic basis for the origin of these strains in humans, the relative frequencies of different serotypes among bovine rotaviruses (BRVs) isolated from southern, western and central regions of the country were determined by subgroup and serotype analysis as well as nucleotide (nt) sequence analysis of the genes encoding the outer capsid proteins VP4 and VP7. Since the human G10P11 asymptomatic neonatal strain I321 possessed NSP1 from a human rotavirus, to determine its genetic origin in the bovine strains, comparative analysis of partial gene sequences from representative G10P11 strains was also carried out. The following observations were of great epidemiological significance, (i) G10P11 strains predominated in all the three regions with frequencies ranging between 55.6% and 85.2%. In contrast to the high prevalence of G6 strains in other countries, only one G6 strain was detected in this study and G8 strains represented 5.8% of the isolates, (ii) among the G10 strains, in serotyping ELISA, four patterns of reactivity were observed that appeared to correlate with the differences in electropherotypic patterns and amino acid (aa) sequence of the VP7, (iii) surprisingly, strains belonging to serotype G3 were detected more frequently (10.7%) than those of serotypes G6 and G8 combined, while strains representing the new serotype (G15) were observed in a single farm in Bangalore, and (iv) about 3.9% of the isolates were nontypeable as they exhibited high cross-reactivity to the serotyping MAbs used in the study. Comparative analysis of the VP7 gene sequence from the prototype G3 MAb-reactive bovine strain J63 revealed greatest sequence relatedness (87.6% nt and 96.0% aa) with that of serotype G3 rhesus-monkey strain RRV. It also exhibited high sequence homology with the VP7 from several animal and animal rotavirus-related human G3 strains (Simian SA11; equine ERV316 and FI-14; canine CU-1 and K9; porcine 4F; Feline Cat2 and human HCR3, YO and AU1). Partial nucleotide sequence analysis of the NSP1 gene of J63 showed greatest nt sequence homology (95.9%) to the NSP1 gene allele of the Indian G8 strain, isolated from a diarrheic child, which is likely to have been transmitted directly from cattle and 92.6% homology to that of the bovine G8 strain A5-10 suggesting the likely origin of J63 by gene reassortment between a bovine G8 strain and a G3 animal strain. Prevalence of G10P11 strains in cattle and G10P11 or P11 type reassortant strains in asymptomatic neonates as well as detection of G8P[1] strains in diarrheic children support our hypothesis for bidirectional transmission of rotaviruses between humans and cattle and origin of novel strains catalyzed by the age-old traditions and socio-economic conditions in India.

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Section: Introductionmentioning

confidence: 99%

Prevalence of, and antigenic variation in, serotype G10 rotaviruses and detection of serotype G3 strains in diarrheic calves: Implications for the origin of G10P11 or P11 type reassortant asymptomatic strains in newborn children in India

Varshney

Jagannath

Vethanayagam

et al. 2002

Archives of Virology

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“…Viruses classified as group A rotavirus (RV-A), including BoRV-A, possess two independent neutralization antigens, namely, VP4 and VP7, on the outer capsid, that specify the P type (for proteasesensitive protein) and G type (for glycoprotein), respectively (Estes, 2001;Kapikian et al, 2001). To date, six P types (P6[1], P7 [5], P8[11], P11[14], P[17], and P [21]) and 8 G types (G1, G3, G5, G6, G7, G8, G10 and G15) have been reported among BoRV-A (Matsuda et al, 1990;Blackhall et al, 1992;Brüssow et al, 1992;Isegawa et al, 1994;Fukai et al, 1999Fukai et al, , 2005Rao et al, 2000;Alfieri et al, 2004). Types G2 and G11 in BoRV-A have been detected using PCRderived cDNA probes (Hussein et al, 1993).…”

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Molecular characterization of a novel bovine group A rotavirus

Fukai

Takahashi

Tajima

et al. 2007

Veterinary Microbiology

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In this study, by partial sequence analysis of the genome segments encoding VP5* and VP7, we characterized a novel bovine group A rotavirus, namely, Tak2, that was detected from adult cattle diarrhea in Tochigi Prefecture, Japan. The nucleotide (nt) and deduced amino acid (aa) sequences of the genome segments encoding VP5* and half of the amino terminal portion of VP7 of Tak2 revealed a low identity with those of group A rotaviruses carrying previously published P and G type specificities (VP5*: nt identity, 61.6%-67.6% and aa identity, 58.0%-71.4%; half of the amino terminal portion of VP7: nt identity, 57.8%-73.5% and aa identity, 61.2%-70.9%). Additionally, phylogenetic analysis of the nt sequences of the genome segments encoding VP5* and half of the amino terminal portion of VP7 revealed that Tak2 formed a branch separate from the established P and G types. These results suggested that Tak2 could possess novel P and G types yet not reported among group A rotaviruses.

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“…Furthermore, they recently proposed that the term P serotype should be reserved for the VP4 antigenic relationships, and that the term VP4 genotype should be employed for VP4 sequence relationships [19]. According to their proposal, comparative VP4 sequence analysis identified more than 17 VP4 genotypes in human and animal rotavirus strains [4,5,9,14,15,19,20]. In equine rotaviruses, however, only strains H2 and FI14 were subjected to VP4 sequence determination [9].…”

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confidence: 99%

“…G3, G5, G13 and G14 have been found among equine rotavirus isolates [2,3,10,12,13], although G5, G13 and G14 are rarely observed in foal diarrhea [1]. In contrast, the presence of 17 different P serotypes (or VP4 genotypes) has been elucidated by serological analysis using antisera against baculovirus-expressed VP4 proteins or by VP4 sequence analysis [4,5,9,14,15,19,20]. In equine rotaviruses, VP4 sequence analysis was applied only to strain H2 and the strain was found to have a distinct VP4 genotype [9].…”

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confidence: 98%