A universal fluorescent sensing system for pathogen determination based on loop-mediated isothermal amplification triggering dual-primer rolling circle extension

Recent advances in cascade isothermal amplification techniques for ultra-sensitive nucleic acid detection

“…RCA is a circular replication of the DNA template proceeding hundreds and even thousands of times by isothermal extension through hybridization of template and primer [ [17] , [18] , [19] ]. In RCA, targets are generally used as primers [ 20 ] or linkers [ 21 ] of padlock probe, which can hinder the improvement of sensitivity to some extent [ 22 ]. Dual primers (forward primer and reverse primer) can greatly improve the reaction efficiency with exponential amplification process, contributable to branched rolling circle amplification (BRCA) [ 23 ] and hyperbranched rolling circle amplification (HRCA) [ 24 ].…”

“…Dual primers (forward primer and reverse primer) can greatly improve the reaction efficiency with exponential amplification process, contributable to branched rolling circle amplification (BRCA) [ 23 ] and hyperbranched rolling circle amplification (HRCA) [ 24 ]. Nevertheless, compared with double stranded DNA (dsDNA) products, single stranded DNA (ssDNA) products of RCA are preferable depending on the design of circular DNA template [ 22 ], which is affected by false-positive results caused by off-template polymerase products, contaminants, and side-reactions [ 1 , 25 ].…”

“…Copyright © 2018 Elsevier B·V. (D) Mechanism of RCA-LAMP fluorescence detection [ 22 ]. Copyright © 2021 Elsevier B·V.…”

Recent advances in biological detection with rolling circle amplification: design strategy, biosensing mechanism, and practical applications

“…This method used the LAMP technology to replace the cyclization process of the lock probe and shorten the amplification time. 102 Similarly, Sun et al 103 engaged the ability of metal–organic skeleton compounds to adsorb single-stranded DNA and quench fluorescence. Fluorescence quenching and recovery can be achieved by adsorption and desorption of single-stranded DNA, which can be combined with RCA to detect EScherichia coli O157:H7 ( E. coli O157:H7) (Fig.…”

A cascade amplification strategy based on rolling circle amplification and hybridization chain reaction for ultrasensitive detection of pathogens

“…As a result, even a 10 aM miRNA target can be clearly and accurately detected. In another study, Chen et al 31 combined LAMP with RCA (LAMP-RCA) through the strand displacement strategy to determine pathogens. The LOD for S. typhimurium was calculated to be 32 CFU μL −1 .…”