A Vaccine against Avian Colibacillosis Based on Ultrasonic Inactivation of Escherichia coli

Melamed, Doron; Leitner, G.; Ed, Heller

doi:10.2307/1591289

Cited by 61 publications

(28 citation statements)

References 8 publications

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“…Vaccines against various E. coli serogroups have been produced (1,2,6,7,11,12,14,15,25,27,29,30,32,36,41,45,46,47). The composition of these vaccines has been quite variable and has included live, attenuated E. coli strains (1,25,29,36), inactivated E. coli strains (11,12,32), active immunization with various E. coli antigens (7,30,41,46,47), and passive immunization (2,6,26). While many of these vaccines were effective against homologous challenge, they did not offer broad-range protection against heterologous challenge.…”

Section: Discussionmentioning

confidence: 99%

Recombinant Iss as a Potential Vaccine for Avian Colibacillosis

et al. 2012

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Section: Discussionmentioning

confidence: 99%

Recombinant Iss as a Potential Vaccine for Avian Colibacillosis

et al. 2012

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“…There are reports describing the use of various inactivated vaccine preparations which resulted in some level of protection of chicks against colibacillosis (Deb & Harry, 1978;Melamed et al, 1991) and as did a multivalent pilus vaccine (Gyimah et al, 1986). On the other hand, live vaccines have been found to induce a higher level of immunity (Arp, 1980;Kwaga et al, 1994).…”

Section: Discussionmentioning

confidence: 99%

Vaccination of turkey poults against pathogenicEscherichia coli

1997

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SUMMARYA vaccine made of bacterial membranes which form vesicles has been developed in our laboratory and found to be effective in protecting chickens against challenge with pathogenic E. coli. In the present study we monitored maternal anti-5. coli antibody in turkey poults and found that although at hatch the antibody titre was relatively high, it decreased gradually to a low level by 14 days of age and remained low up to 31 days of age. Both intramuscular and subcutaneous vaccination with membrane vesicles (MV) resulted in increased antibody titres. Poults with high antibody titres could survive challenge with the pathogenic bacteria. Serum from vaccinated poults reduced E. coli bacterial growth rate when added to the growth medium at a high concentration, whereas serum from non-vaccinated poults did not. Lymphocytes from vaccinated poults responded with an increased rate of blastogenesis when MV was added to the cultures. Lymphocytes from non-vaccinated poults were not stimulated to blastogenesis. We concluded that MV can be used as a vaccine to render poults resistant to E. coli by evoking antibody production, stimulating the bacterial-lysis activity of complement, stimulating T cells to proliferate and stimulating cytotoxic T cells involved in disease resistance.

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“…Chickens with greater IgG, IgA and IgM responses to selected cell surface antigens of APEC were protected against experimental challenge than their unvaccinated counterparts (Kariyawasam et al, 2002). Correlation between the antibody titre and lesions was observed following vaccination of chickens with an ultrasonic inactivationbased E. coli vaccine (Melamed et al, 1991). Even passively transferred antibodies protected against homologous challenge with respective virulent APEC strains (Arp, 1980;Bolin & Jensen, 1987;Kariyawasam et al, 2004a).…”

Section: Prospects For the Identification Of Novel Apec Virulence Detmentioning

confidence: 99%

Colibacillosis in poultry: unravelling the molecular basis of virulence of avian pathogenicEscherichia coliin their natural hosts

Dziva¹,

Stevens²

2008

Avian Pathology

305

222

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Avian colibacillosis is caused by a group of pathogens designated avian pathogenic Escherichia coli (APEC). Despite being known for over a century, avian colibacillosis remains one of the major endemic diseases afflicting the poultry industry worldwide. Autologous bacterins provide limited serotype-specific protection, yet multiple serogroups are associated with disease, especially O1, O2 and O78 among many others. Experimental infection models have facilitated the identification of some key APEC virulence genes and have allowed testing of vaccine candidates. Well-recognized virulence factors include Type 1 (F1) and P (Pap/ Prs) fimbriae for colonization, IbeA for invasion, iron acquisition systems, TraT and Iss for serum survival, K and O antigens for anti-phagocytic activity, and a temperature-sensitive haemagglutinin of imprecise function. Intriguingly, these factors do not occur universally among APEC, suggesting the presence of multiple alternative mechanisms mediating pathogenicity. The recent availability of the first complete APEC genome sequence can be expected to accelerate the identification of bacterial genes expressed during infection and required for virulence. High-throughput molecular approaches like signature-tagged transposon mutagenesis have already proved invaluable in revealing portfolios of genes expressed by pathogenic bacteria during infection, and this has enabled identification of APEC O2 factors required for septicaemia in the chicken model. Complimentary approaches, such as in vivo-induced antigen technology, exist to define the activities of APEC in vivo. In recent years, reverse vaccinology and immuno-proteomic approaches have also enabled identification of novel vaccine candidates in other bacterial pathogens. Collectively, such information provides the basis for the development or improvement of strategies to control APEC infections in the food-producing avian species.

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A Vaccine against Avian Colibacillosis Based on Ultrasonic Inactivation of Escherichia coli

Cited by 61 publications

References 8 publications

Recombinant Iss as a Potential Vaccine for Avian Colibacillosis

Recombinant Iss as a Potential Vaccine for Avian Colibacillosis

Vaccination of turkey poults against pathogenicEscherichia coli

Colibacillosis in poultry: unravelling the molecular basis of virulence of avian pathogenicEscherichia coliin their natural hosts

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