A validated eco-friendly HPLC-FLD for analysis of the first approved antiviral remdesivir with other potential add-on therapies for COVID-19 in human plasma and pharmaceuticals

Abstract

An HPLC-FLD method was proposed to determine remdesivir, hydroxychloroquine, and levofloxacin in their pure form and in human plasma, with high sensitivity and a wide linear range. Validation was done as per ICH and FDA guidelines, and the method greenness was evaluated.