A versatile SERS-based immunoassay for immunoglobulin detection using antigen-coated gold nanoparticles and malachite green-conjugated protein A/G

A Fast and Cost-Effective Detection of Melamine by Surface Enhanced Raman Spectroscopy Using a Novel Hydrogen Bonding-Assisted Supramolecular Matrix and Gold-Coated Magnetic Nanoparticles

“…As Fe3O4 nanoparticle and AOA are tens of thousands of times less expensive than monoclonal antibodies used in traditional sandwich immunoassays, and the total analysis time of the assay was less than 1 h, the current method affords promise as a biosensor platform that can be adapted for portable and cost-effective detection applications. In a traditional enzyme-linked immunosorbent assay (ELISA) or the immuno-based SERS detection of melamine, monoclonal or polyclonal antibodies are inevitably used to establish the assay [14,27,28]. In comparison with commercial Fe 3 O 4 nanoparticles, 5-aminoorotic acid, and Au nanoparticles, these antibodies are often tens of thousands of times more expensive and require careful handling and disposal because they are sensitive to temperature and pH value.…”

“…The regression of the logarithmic peak intensity at 1510 cm −1 versus the melamine concentration produced a linear plot with linear equation as lgI = 2.02148 + 0.05852 × C (lgI represents the logarithms of the peak intensity at 1510 cm −1 , C is the melamine concentrations) and R 2 = 0.9852 in the melamine concentration ranging from 2.5 to 15.0 µg/mL. In a traditional enzyme-linked immunosorbent assay (ELISA) or the immuno-based SERS detection of melamine, monoclonal or polyclonal antibodies are inevitably used to establish the assay [14,27,28]. In comparison with commercial Fe3O4 nanoparticles, 5-aminoorotic acid, and Au nanoparticles, these antibodies are often tens of thousands of times more expensive and require careful handling and disposal because they are sensitive to temperature and pH value.…”

Biotechnological tools for diagnosis of equine infectious diseases

“…West nile virus was detected using biosensors and microfluidic systems, a linear, 15 amino acid fragment of domain III of WNV was successfully used as an antigen on an amperometric immunosensor (Ionescu et al, 2007). Neng et al (2010) reported that, a surface enhanced Raman scattering immunoassay was shown to be highly sensitive for the detection of anti-WNV immunoglobulin. Hu et al (2004) developed a genetically biotinylated single chain fragment variable antibody (scFv) against Venezuelan equine encephalitis virus (VEE).…”

Surface‐Enhanced Raman Scattering‐Based Bioimaging

“…The labeled approach is highly specific because it exhibits little to no signal interference from other Raman-active species as the SERS enhancement decays exponentially from the surface of the nanoparticles, making any other contributions virtually undetectable compared to the Raman-active molecules adsorbed on the nanoparticle surface. gold nanostructures with Raman reporters (called SERS probes henceforth) have been extensively investigated for cancer [18,[32][33][34][35] and other disease [36,37] biomarker detection, as well as bacterial [38][39][40][41] and viral [42,43] microorganism [42][43][44] detection. common with any other optical bioimaging modality, which employs exogenous contrast agents, ultrabright probes are paramount for deep and high-resolution SERS-based bioimaging.…”