A visual detection strategy for SARS-CoV-2 based on dual targets-triggering DNA walker

Zhao, Le; Li, Ciling; Li, Yubin

doi:10.1016/j.snb.2022.133252

Cited by 2 publications

(1 citation statement)

References 43 publications

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“…Another approach is the use of a DNA walker that binds to a silver-coated glass slide, with an enzymatic reaction of exonuclease that allows the release of DNA sequences if the target is present, by hybridization and cleavage of the exonuclease. The fluorescence emitted correlates with the amount of target present, and can be quantified using a smartphone [ 106 ].…”

Section: Microfluidics Integration and Nanomedicine Advancesmentioning

confidence: 99%

Detection of SARS-CoV-2 Based on Nucleic Acid Amplification Tests (NAATs) and Its Integration into Nanomedicine and Microfluidic Devices as Point-of-Care Testing (POCT)

Dorta-Gorrín

Navas-Mendez

Gozalo-Margüello

et al. 2023

IJMS

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The coronavirus SARS-CoV-2 has highlighted the criticality of an accurate and rapid diagnosis in order to contain the spread of the virus. Knowledge of the viral structure and its genome is essential for diagnosis development. The virus is still quickly evolving and the global scenario could easily change. Thus, a greater range of diagnostic options is essential to face this threat to public health. In response to the global demand, there has been a rapid advancement in the understanding of current diagnostic methods. In fact, innovative approaches have emerged, leveraging the benefits of nanomedicine and microfluidic technologies. Although this development has been incredibly fast, several key areas require further investigation and optimization, such as sample collection and preparation, assay optimization and sensitivity, cost effectiveness, scalability device miniaturization, and portability and integration with smartphones. Addressing these gaps in the knowledge and these technological challenges will contribute to the development of reliable, sensitive, and user-friendly NAAT-based POCTs for the diagnosis of SARS-CoV-2 and other infectious diseases, facilitating rapid and effective patient management. This review aims to provide an overview of current SARS-CoV-2 detection methods based on nucleic acid detection tests (NAATs). Additionally, it explores promising approaches that combine nanomedicine and microfluidic devices with high sensitivity and relatively fast ‘time to answer’ for integration into point-of-care testing (POCT).

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Section: Microfluidics Integration and Nanomedicine Advancesmentioning

confidence: 99%