A water-soluble polysaccharide from the roots of Eremurus spectabilis M. B. subsp. spectabilis: Extraction, purification and structural features

Beigi, Masoumeh; Jahanbin, Kambiz

doi:10.1016/j.ijbiomac.2019.01.178

Cited by 22 publications

(11 citation statements)

References 40 publications

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“…Intense through-bond resonances between H1 (5.36 ppm), C2 (74.4 ppm), C3 (76.0 ppm), C4 (80.1 ppm), and C5 (74.1 ppm) of α -D-Glc p were observed in the HMBC spectrum ( Figure S4 ), proving the presence of α-1,4-D-glucan (glycogen) [ 70 , 71 ]. The following fragments were also identified: 1,4- β -D-Glc p , 1,4- β -D-Man p , 1,4,6- β -D-Man p , and β -D-Man p [ 72 , 73 ]. Based on inter-glycosidic interactions in the HMBC spectrum, it was found that β -1,4-D-Glc p and β -1,4,6-D-Man p were linked together by 1,4-glycosidic bonds [ 72 ].…”

Section: Resultsmentioning

confidence: 99%

“…The following fragments were also identified: 1,4- β -D-Glc p , 1,4- β -D-Man p , 1,4,6- β -D-Man p , and β -D-Man p [ 72 , 73 ]. Based on inter-glycosidic interactions in the HMBC spectrum, it was found that β -1,4-D-Glc p and β -1,4,6-D-Man p were linked together by 1,4-glycosidic bonds [ 72 ]. A cross-peak was observed between C1 of β -1,4-D-Glc p and H4 of β -1,4,6-D-Man p at δ 105.7/3.84 ppm in the HMBC spectrum.…”

Section: Resultsmentioning

confidence: 99%

“…No definite interactions were found in the 2D spectra to prove that β -1,4,6-D-Man p was replaced at O -6 by Man or the presence of 1,4-linked β -D-homomanan due to the low Man content. Both types of binding exist in glucomannans [ 72 ].…”

Section: Resultsmentioning

confidence: 99%

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Structural Features and Immunomodulatory Effects of Water-Extractable Polysaccharides from Macrolepiota procera (Scop.) Singer

Georgiev

Vašíček

Dzhambazov

et al. 2022

JoF

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Macrolepiota procera (MP) is an edible mushroom used in the treatment of diabetes, hypertension and inflammation. However, the structure and biological effects of its polysaccharides (PSs) are unclear. This study investigates the structural features of a PS complex from MP (MP-PSC), its immunomodulatory activities and effects on probiotic and pathogenic bacteria. MP-PSC was obtained by boiling water, and PSs were characterized by 2D NMR spectroscopy. The immunomodulatory effects on blood and derived neutrophils, other leukocytes, and murine macrophages were studied by flow cytometry, chemiluminescence, spectrophotometry, and ELISA. The total carbohydrate content of MP-PSC was 74.2%, with glycogen occupying 36.7%, followed by β-D-glucan, α-L-fuco-2-(1,6)-D-galactan, and β-D-glucomannan. MP-PSC (200 μg/mL) increased the number of CD14+ monocyte cells in the blood, after ex vivo incubation for 24 h. It dose-dependently (50–200 μg/mL) activated the spontaneous oxidative burst of whole blood phagocytes, NO, and interleukin 6 productions in RAW264.7 cells. MP-PSC exhibited a low antioxidant activity and failed to suppress the oxidative burst and NO generation, induced by inflammatory agents. It (2.0%, w/v) stimulated probiotic co-cultures and hindered the growth and biofilm development of Escherichia coli, Streptococcus mutans and Salmonella enterica. MP PSs can be included in synbiotics to test their immunostimulating effects on compromised immune systems and gut health.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Structural Features and Immunomodulatory Effects of Water-Extractable Polysaccharides from Macrolepiota procera (Scop.) Singer

Georgiev

Vašíček

Dzhambazov

et al. 2022

JoF

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“…The signals at 98.71–104.32 ppm were mainly assigned to C‐1 of SSPS‐N‐b, and the signals at 60.47–66.40 ppm were mainly assigned to C‐6 of SSPS‐N‐b. The HSQC (Figure 5c) results suggested that the signals at 104.32/4.58 ppm (C‐1/H‐1), 77.62/3.60 ppm (C‐4/H‐4) and 60.50/3.86 ppm (C‐6/H‐6) were assigned to β‐1,4‐ d ‐Glc p ; the signals at 100.11/4.69 ppm (C‐1/H‐1), 76.51/3.76 ppm (C‐4/H‐4) and 60.47/3.86 ppm (C‐6/H‐6) were assigned to β‐1,4‐ d ‐Man p ; and the signals at 104.32/4.59 ppm (C‐1/H‐1), 77.59/4.11 ppm (C‐4/H‐4) and 60.65/3.73 ppm (C‐6/H‐6) were assigned to β‐1,4‐ d ‐Gal p (Beigi & Jahanbin, 2019; Chan et al, 2020; Vilaro et al, 2017). However, some weak signals at 4.96/98.71 ppm (C‐1/H‐1) and 66.40/3.91 ppm (C‐6/H‐6) indicated the presence of β‐1,4,6‐ d ‐Man p , and the signal at 70.42/4.00 ppm was attributed to the C‐4/H‐4 of β‐ d ‐Glc p .…”

Section: Resultsmentioning

confidence: 99%

Purification, characterization, and antioxidant activity of polysaccharides from Okara

Wang

Shi

et al. 2022

Food Processing Preservation

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A neutral polysaccharide (SSPS-N) and an acidic polysaccharide (SSPS-A) were isolated from Okara by anion-exchange chromatography. The physical and chemical properties of polysaccharides were studied by HPLC, FT-IR, and SEM. The results showed that SSPS-N might be a galactomannans with a lamella-like structure, and SSPS-A might be an arabinogalactan with a complete porous structure. Furthermore, a new neutral soybean soluble polysaccharide (SSPS-N-b) with a molecular weight of 8.6 kDa was isolated from SSPS-N, and its fine structure was analyzed by GC-MS, enzymatic analysis, and NMR. The results showed that SSPS-N-b was a mixture of β-1,4galactan and glucomannan, which (1→4)βd-linked mannose and (1→4)βd-linked glucose were connected alternately, and it was in parallel with β-1,4-galactan. The antioxidant activity results showed that β-1,4-Galactan in SSPS-N-b was identified as the main activity domain. This result will be utilized for the development of SSPS as novel functional foods and medicine.

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“…The collected fractions were monitored by the phenol–sulfuric acid colorimetric method at 490 nm (Dubois, Gilles, Hamilton, Rebers, & Smith, F., 1956). Fractions, which corresponded to the major peak, were pooled, dialyzed, and lyophilized to result white pure polysaccharide (RMEP) and used for further study (Beigi & Jahanbin, 2019).…”

Section: Methodsmentioning

confidence: 99%

Production, medium optimization, and structural characterization of an extracellular polysaccharide produced byRhodotorula minuta ATCC 10658

Samadlouie

Jahanbin

jalali

2020

Food Science & Nutrition

Self Cite

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Several strains of microorganism are capable of converting carbohydrates into extracellular polysaccharide. The preset research is a first effort made to optimize extracellular polysaccharide (CRMEP) by Rhodotorula minuta ATCC 10658 using one factor at time and response surface methods. One factor at time was applied in the initial screening of substrates prior to optimization study. Of all the substrates examined, starch as carbon source and defatted soy bean powder as protein source were discovered to be best for CRMEP production. Response surface analysis revealed that 15 g/L starch and 30g/L defatted soy bean powder were the optimal chemical conditions. The model predicted 13.22 g/L for CRMEP, which went along with the experimentally observed result. Purification of CRMEP by anion‐exchange column of DEAE‐cellulose yielded RMEP. Structural investigation indicated that the main chain of RMEP was composed of (1 → 3) and (1 → 4)‐linked mannopyranosyl residues, with branches attached to O‐6 of some (1 → 3)‐linked mannopyranosyl residues. The branches were composed of Glcp‐(1 → residues.

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A water-soluble polysaccharide from the roots of Eremurus spectabilis M. B. subsp. spectabilis: Extraction, purification and structural features

Cited by 22 publications

References 40 publications

Structural Features and Immunomodulatory Effects of Water-Extractable Polysaccharides from Macrolepiota procera (Scop.) Singer

Structural Features and Immunomodulatory Effects of Water-Extractable Polysaccharides from Macrolepiota procera (Scop.) Singer

Purification, characterization, and antioxidant activity of polysaccharides from Okara

Production, medium optimization, and structural characterization of an extracellular polysaccharide produced byRhodotorula minuta ATCC 10658

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