A wheat caffeic acid 3-O-methyltransferase TaCOMT-3D positively contributes to both resistance to sharp eyespot disease and stem mechanical strength

Wang, Minxia; Zhu, Xinna; Wang, Ke; Lu, Chungui; Luo, Meiying; Shan, Tianlei; Zhang, Zengyan

doi:10.1038/s41598-018-24884-0

Cited by 77 publications

(50 citation statements)

References 54 publications

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“…Nevertheless, duplication seems to have been followed by some divergence in expression pattern, possibly reflecting subfunctionalization in different tissues or cell types (Ober, ). Several COMT s previously identified in wheat (Jung et al ., ; Ma and Xu, ; Wang et al ., ) are homologues of the barley COMT genes. COMT duplication events have also been noted in ryegrass ( Lolium perenne ) (van Parijs et al ., ).…”

Section: Discussionmentioning

confidence: 99%

“…Barley, brachypodium and rice sequences with >40% identity to maize ZmCOMT (M73235) (Collazo et al, 1992) were retrieved from sequence databases and used for phylogenetic analysis along with published COMT genes from perennial ryegrass (Heath et al, 1998), sorghum (Bout and Vermerris, 2003), alfalfa (Zubieta et al, 2002), switchgrass (Fu et al, 2011), sugarcane (Jung et al, 2012;Selman-Housein et al, 1999), arabidopsis COMT and COMT-like genes from (Raes et al, 2003), several wheat COMT or OMT genes (Jung et al, 2008;Ma and Xu, 2008;Wang et al, 2018), and two Clarkia brewer genes (Wang and Pichersky, 1999). After importing aligned sequences into MEGA7, a maximum likelihood (ML) tree was constructed with JTT (Jones et al, 1992) + G + I as the model with five discrete gamma categories.…”

Section: Sequence Retrieval and Phylogenetic Analysismentioning

confidence: 99%

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RNAi‐suppression of barley caffeic acidO‐methyltransferase modifies lignin despite redundancy in the gene family

Daly

McClellan

Maluk

et al. 2018

Plant Biotechnology Journal

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Caffeic acid O-methyltransferase (COMT), the lignin biosynthesis gene modified in many brown-midrib high-digestibility mutants of maize and sorghum, was targeted for downregulation in the small grain temperate cereal, barley (Hordeum vulgare), to improve straw properties. Phylogenetic and expression analyses identified the barley COMT orthologue(s) expressed in stems, defining a larger gene family than in brachypodium or rice with three COMT genes expressed in lignifying tissues. RNAi significantly reduced stem COMT protein and enzyme activity, and modestly reduced stem lignin content while dramatically changing lignin structure. Lignin syringyl-to-guaiacyl ratio was reduced by ~50%, the 5-hydroxyguaiacyl (5-OH-G) unit incorporated into lignin at 10--15-fold higher levels than normal, and the amount of p-coumaric acid ester-linked to cell walls was reduced by ~50%. No brown-midrib phenotype was observed in any RNAi line despite significant COMT suppression and altered lignin. The novel COMT gene family structure in barley highlights the dynamic nature of grass genomes. Redundancy in barley COMTs may explain the absence of brown-midrib mutants in barley and wheat. The barley COMT RNAi lines nevertheless have the potential to be exploited for bioenergy applications and as animal feed.

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Section: Discussionmentioning

confidence: 99%

Section: Sequence Retrieval and Phylogenetic Analysismentioning

confidence: 99%

RNAi‐suppression of barley caffeic acidO‐methyltransferase modifies lignin despite redundancy in the gene family

Daly

McClellan

Maluk

et al. 2018

Plant Biotechnology Journal

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“…In a citrus breeding program, this could be utilized for early selection of seedlings in the juvenile phase, which are expected to accumulate a higher amount of PMFs in fruit, resulting in a shortening of the breeding period. The OMT multigene family has been identified in many plant species, including alfalfa (Maxwell et al, 1993), American golden saxifrage (Gauthier et al, 1995), Arabidopsis (Zhang et al, 1997), barley (Christensen et al, 1998;Zhou et al, 2008), corn (Zhou et al, 2008), Madagascar periwinkle (Cacace et al, 2003), mango (Chidley et al, 2016), peppermint (Willits et al, 2004), rice (Kim et al, 2006), vanilla (Li et al, 2006), and wheat (Wang et al, 2018;Zhou et al, 2006). Recently, five OMT genes for methylation of flavones were isolated from Shiikuwasha (Itoh et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Accumulation of Polymethoxyflavones and <i>O</i>-methyltransferase Gene Expression in Various Citrus Cultivars

et al. 2020

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Polymethoxyflavones (PMFs) are naturally occurring O-methylated flavones predominantly accumulated in different citrus tissues that show numerous human-health promoting activities. O-methyltransferase (OMT) family members are involved in the biosynthesis of PMFs in plant species including citrus. Although the distribution of PMFs has been determined in citrus cultivars, the relation between the accumulation of PMFs and the related OMT gene expression in leaves and flavedos (exocarps) of citrus cultivars remains to be clarified. In this study, we investigated the accumulation of nobiletin and tangeretin in the leaves and flavedos of various citrus cultivars widely grown in Japan. As expected, the accumulation of nobiletin and tangeretin varied among the citrus cultivars and the two tissues, and nobiletin and tangeretin accumulated at higher levels in flavedos than in leaves. In particular, we clearly demonstrated that the nobiletin content showed a significant and positive correlation (r = 0.824, P = 0.00182) between leaves and flavedos. Based on these results, the concentration of PMFs in leaves could be utilized as an early selection marker for seedlings in the juvenile phase that are expected to accumulate a higher amount of PMFs in fruit, resulting in a shortening of the breeding period. We also identified two novel candidate genes, CreOMT1 and CreOMT4, putatively involved in PMF biosynthesis in citrus. Notably, the expression of CreOMT1 showed a significant correlation (r = 0.700, P = 0.0243) with the nobiletin content in the flavedos of 10 citrus cultivars. Our results provide valuable information for developing new citrus cultivars that could accumulate higher PMFs and insight into elucidating the PMF biosynthetic pathway in citrus in the future.

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“…The SAM-dependent methyltransferases genes were identi ed to play important role in sweet potato (Ipomoea batatas) in response to salt stress (Liu et al 2015). In wheat, TaCOMT-3D contributes to stem mechanical support (Wang et al 2018). Another TaCOMT gene was also observed with constitutive expression in stem along with leaf and root (Ma 2009).…”

Section: Omts Are Involved In Plant Growth Abiotic Stress Tolerancementioning

confidence: 99%

Biodiverse Responses of O-methyltransferase Genes to Salt Stress and Fiber Development of Gossypium Species

Hafeez

Gě

Zhāng

et al. 2020

Preprint

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O-methyltransferases (OMTs) are an important group of enzymes that catalyze the transfer of a methyl group from S-adenosyl-L-methionine to their acceptor substrates. OMTs are divided into several groups according to their structural features. In Gossypium species, they are involved in phenolics and flavonoid pathways. Phenolics defend the cellulose fiber from dreadful external conditions of biotic and abiotic stresses, promoting strength and growth of plant cell wall. In this study, an OMT gene family, containing a total of 192 members, has been identified and characterized in three main Gossypium species, G. hirsutum, G. arboreum and G. raimondii. Cis-regulatory elements analysis suggested important roles of OMT genes in growth, development, and defense against stresses. Transcriptome data of different fiber developmental stages in Chromosome Substitution Segment Lines (CSSLs), Recombination Inbred Lines (RILs) with excellent fiber quality, and standard genetic cotton cultivar TM-1 demonstrate that up-regulation of OMT genes at different fiber developmental stages, and abiotic stress treatments have some significant correlations with fiber quality formation, and with salt stress response. Quantitative RT-PCR results revealed that GhOMT43 and GhOMT27 genes had a specific expression in response to salt stress while GhOMT16, GhOMT55, and GhOMT33 in fiber elongation and secondary cell wall stages. Our results indicated that these genes might contribute to salt tolerance or fiber quality traits respectively in Gossypium.

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A wheat caffeic acid 3-O-methyltransferase TaCOMT-3D positively contributes to both resistance to sharp eyespot disease and stem mechanical strength

Cited by 77 publications

References 54 publications

RNAi‐suppression of barley caffeic acidO‐methyltransferase modifies lignin despite redundancy in the gene family

RNAi‐suppression of barley caffeic acidO‐methyltransferase modifies lignin despite redundancy in the gene family

Accumulation of Polymethoxyflavones and <i>O</i>-methyltransferase Gene Expression in Various Citrus Cultivars

Biodiverse Responses of O-methyltransferase Genes to Salt Stress and Fiber Development of Gossypium Species

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