A wheat genomic DNA fragment reduces pollen transmission of maize transgenes by reducing pollen viability

Scott, M. Paul; Peterson, J. M.; Moran, Daniel L.; Sangtong, Varaporn; Smith, LaTrice

doi:10.1007/s11248-006-9055-x

Cited by 5 publications

(3 citation statements)

References 26 publications

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“…Similarly, differential pollen transmission of the transgene between opaque and translucent kernels could result in a similar underrepresentation of the transgene in the opaque pool. While transgenes have been observed to exhibit abnormal pollen transmission (Scott et al, 2007), it is unlikely that the degree of pollen transmission would be altered by opaque2 , a mutation that primarily effects seed phenotypes.…”

Section: Resultsmentioning

confidence: 99%

Maize Transgenes Containing Zein Promoters are Regulated by opaque2

2011

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Understanding epistatic interactions between transgenes and native genes is critical for predicting the response of transgenes to different genetic backgrounds and environments. Our objective was to determine if predicted epistatic interactions occur between maize (Zea mays L.) transgenes carrying zein promoters and opaque2, a transcriptional regulator of zein genes. Expression of the transgenes was significantly decreased in kernels containing the opaque2 mutation. Native zein proteins in these kernels were reduced as well. Thus, transgene expression mirrored expression of the native gene that contributed the promoter of the transgene. This work demonstrates that information about native gene expression may be useful for predicting transgene expression, reducing the risk of unintended consequences of transgene expression.

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Section: Resultsmentioning

confidence: 99%

Maize Transgenes Containing Zein Promoters are Regulated by opaque2

2011

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“…Twenty randomly selected sibling kernels from one ear representing each event were photographed and volume and weight measurements were taken for estimates of density. Following measurements, kernels were screened by dot blot immuno assay as described previously (Scott et al 2007) to identify which kernels contained a-lactalbumin.…”

Section: Evaluation Of Kernel Phenotypesmentioning

confidence: 99%

“…Another limitation of plant proteins is that they can be poorly digested by animals or can cause allergic reactions (Nordlee et al 1996;Scott and Bicar 2003). Milk proteins have been proposed as alternative proteins for expression in plants for nutritional improvement (Chong et al 1997;Yang et al 2002) because they are a natural component of animal diets and they have excellent nutritional parameters (Renner 1988;Matthews and Hughes 1993).…”

Section: Introductionmentioning

confidence: 99%

Transgenic maize endosperm containing a milk protein has improved amino acid balance

et al. 2007

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In order to meet the protein nutrition needs of the world population, greater reliance on plant protein sources will become necessary. The amino acid balance of most plant protein sources does not match the nutritional requirements of monogastric animals, limiting their nutritional value. In cereals, the essential amino acid lysine is deficient. Maize is a major component of human and animal diets worldwide and especially where sources of plant protein are in critical need such as sub-Saharan Africa. To improve the amino acid balance of maize, we developed transgenic maize lines that produce the milk protein α-lactalbumin in the endosperm. Lines in which the transgene was inherited as a single dominant genetic locus were identified. Sibling kernels with or without the transgene were compared to determine the effect of the transgene on kernel traits in lines selected for their high content of α-lactalbumin. Total protein content in endosperm from transgene positive kernels was not significantly different from total protein content in endosperm from transgene negative kernels in three out of four comparisons, whereas the lysine content of the lines examined was 29-47% greater in endosperm from transgene positive kernels. The content of some other amino acids was changed to a lesser extent. Taken together, these changes resulted in the transgenic endosperms having an improved amino acid balance relative to non-transgenic endosperms produced on the same ear. Kernel appearance, weight, density and zein content did not exhibit substantial differences in kernels expressing the transgene when compared to non-expressing siblings. Assessment of the antigenicity and impacts on animal health will be required in order to determine the overall value of this technology. Abstract In order to meet the protein nutrition needs of the world population, greater reliance on plant protein sources will become necessary. The amino acid balance of most plant protein sources does not match the nutritional requirements of monogastric animals, limiting their nutritional value. In cereals, the essential amino acid lysine is deficient. Maize is a major component of human and animal diets worldwide and especially where sources of plant protein are in critical need such as sub-Saharan Africa. To improve the amino acid balance of maize, we developed transgenic maize lines that produce the milk protein a-lactalbumin in the endosperm. Lines in which the transgene was inherited as a single dominant genetic locus were identified. Sibling kernels with or without the transgene were compared to determine the effect of the transgene on kernel traits in lines selected for their high content of a-lactalbumin. Total protein content in endosperm from transgene positive kernels was not significantly different from total protein content in endosperm from transgene negative kernels in three out of four comparisons, whereas the lysine content of the lines examined was 29-47% greater in endosperm from transgene positive kernels. The content of some other...

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Characterizing Transgene Inheritance

Bicar

2009

Methods in Molecular Biology

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The analysis of transgene inheritance is an important step in the molecular and genetic characterization of transgenes. In this manuscript, two approaches to characterize the inheritance of transgenes are described. The first approach is based on the expression of the transgene phenotype and the second is based on the analysis of transgene DNA. Instructions on how to make crosses and develop breeding populations are outlined and the importance of these breeding populations in the analysis of transgene inheritance is explained. The number of individuals needed to determine segregation ratios and the statistic used to test these ratios are described. Examples of inheritance patterns that deviate from known expectations are provided and the possible causes of these deviations are discussed.

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A wheat genomic DNA fragment reduces pollen transmission of maize transgenes by reducing pollen viability

Cited by 5 publications

References 26 publications

Maize Transgenes Containing Zein Promoters are Regulated by opaque2

Maize Transgenes Containing Zein Promoters are Regulated by opaque2

Transgenic maize endosperm containing a milk protein has improved amino acid balance

Characterizing Transgene Inheritance

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