A workflow pipeline to distinguish the ambiguous umbilical cord bloods of boy–girl twins

Cai, Jing; Wan, Qian

doi:10.1007/s13577-022-00803-4

Cited by 2 publications

(1 citation statement)

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“…This criterion was based on the basic fact that CXCR3 is on the X chromosome and TSPY1 is on the Y chromosome. The detailed principles of this sex determination method was published before [10][11][12].Based on the criteria and data, it was easy to distinguish male samples from female samples. In addition, the sex type of two Klinefelter syndrome positive samples was judged to be male (Table 4 was the example).…”

Section: Resultsmentioning

confidence: 99%

A method of screening for Klinefelter syndrome by detecting amniotic fluid punctures

Xiang,

Wan

2023

Preprint

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We aimed to develop a new method of screening for Klinefelter Syndrome by detecting amniotic fluid punctures to complement the current methods. Two commercially available genomic DNA extracted from the amniotic fluid puncture of the pregnant woman with the Klinefelter syndrome fetus, two genomic DNAs extracted from two healthy females and four genomic DNAs extracted from four healthy males were used as the qPCR template DNAs and the commercially available Sybr green qPCR master mix were used; we designed and synthesized 5 pairs of qPCR primers respectively corresponding to IL-10 gene on 1# chromosome, STAT1 gene on 2# chromosome, CXCR3 gene on X chromosome, TSPY1 gene on Y chromosome and LINC00458 on 13# chromosome. We then performed Sybr green qPCR measurement. We processed the qPCR data by mathematical calculation and finally formed a new algorithm. Using the new algorithm, we easily distinguished the Klinefelter syndrome samples out of the normal male samples. We developed a new method of screening for Klinefelter syndrome for the male fetus by detecting amniotic fluid punctures to complement the current methods.

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Section: Resultsmentioning

confidence: 99%