A yeast assay based on the gilthead sea bream (teleost fish) estrogen receptor β for monitoring estrogen mimics

Passos, Ana Lúcia; Pinto, Patrícia I. S.; Power, Deborah M.; Canário, Adelino V.M.

doi:10.1016/j.ecoenv.2009.02.004

Cited by 12 publications

(7 citation statements)

References 40 publications

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“…Furthermore, both E 2 and EE 2 have slightly greater binding affinity for the Esr1 subtype compared with Esr2a and Esr2b as described in previous fish studies (Gale et al, 2004;Passos et al, 2009). However, Esr binding studies performed in fish and other species generally agree that the relative binding affinity (RBA) for EE2 can be greater (range of 89-647) than E2 (100) (Denny et al, 2005;Gale et al, 2004;Passos et al, 2009;Shyu et al, 2011); therefore, it is somewhat surprising that EE2 does not produce a greater response (repression of vtgr-Luc activity) based on binding affinity alone. In addition to RBA, there are a number of other factors in play including saturation of the Esrs, differential recruitment of co-regulatory proteins, and cross-talk between the Esrs and other pathways that likely contribute to the observed results.…”

Section: Discussionsupporting

confidence: 67%

Control of Transcriptional Repression of the Vitellogenin Receptor Gene in Largemouth Bass (Micropterus Salmoides) by Select Estrogen Receptors Isotypes

Domínguez

Bisesi²,

Kroll

et al. 2014

Toxicological Sciences

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The vitellogenin receptor (Vtgr) plays an important role in fish reproduction. This receptor functions to incorporate vitellogenin (Vtg), a macromolecule synthesized and released from the liver in the bloodstream, into oocytes where it is processed into yolk. Although studies have focused on the functional role of Vtgr in fish, the mechanistic control of this gene is still unexplored. Here we report the identification and analysis of the first piscine 5' regulatory region of the vtgr gene which was cloned from largemouth bass (Micropterus salmoides). Using this putative promoter sequence, we investigated a role for hormones, including insulin and 17β-estradiol (E2), in transcriptional regulation through cell-based reporter assays. No effect of insulin was observed, however, E2 was able to repress transcriptional activity of the vtgr promoter through select estrogen receptor subtypes, Esr1 and Esr2a but not Esr2b. Electrophoretic mobility shift assay demonstrated that Esr1 likely interacts with the vtgr promoter region through half ERE and/or SP1 sites, in part. Finally we also show that ethinylestradiol (EE2), but not bisphenol-A (BPA), represses promoter activity similarly to E2. These results reveal for the first time that the Esr1 isoform may play an inhibitory role in the expression of LMB vtgr mRNA under the influence of E2, and potent estrogens such as EE2. In addition, this new evidence suggests that vtgr may be a target of select endocrine disrupting compounds through environmental exposures.

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Section: Discussionsupporting

confidence: 67%

Control of Transcriptional Repression of the Vitellogenin Receptor Gene in Largemouth Bass (Micropterus Salmoides) by Select Estrogen Receptors Isotypes

Domínguez

Bisesi²,

Kroll

et al. 2014

Toxicological Sciences

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“…In contrast, the exposure of goldfish scales to E 2 upregulated both transcripts [37], and PCB 118 upregulated cathepsin K, TRAP and RANKL [92]. Several of these contaminants have previously been shown or predicted to bind and/or activate fish ERs (e.g., [87,93,94,95]), suggesting that part of the effect of these EDCs is via a direct action on intracellular ER expression in fish scales.…”

Section: Estrogenic Endocrine Disruption In Mineralized Tissuesmentioning

confidence: 99%

“…Moreover, many EDCs already have identified disruptive actions on fish reproductive tissues and bind and/or transactivate fish ERs (e.g., [16,78,87,94,99,100,101,102,103]), and together, these may be good starting candidates to investigate the impact of EDCs in fish mineralized tissues.…”

Section: Estrogenic Endocrine Disruption In Mineralized Tissuesmentioning

confidence: 99%

Effects of Estrogens and Estrogenic Disrupting Compounds on Fish Mineralized Tissues

2014

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Estrogens play well-recognized roles in reproduction across vertebrates, but also intervene in a wide range of other physiological processes, including mineral homeostasis. Classical actions are triggered when estrogens bind and activate intracellular estrogen receptors (ERs), regulating the transcription of responsive genes, but rapid non-genomic actions initiated by binding to plasma membrane receptors were recently described. A wide range of structurally diverse compounds from natural and anthropogenic sources have been shown to interact with and disrupt the normal functions of the estrogen system, and fish are particularly vulnerable to endocrine disruption, as these compounds are frequently discharged or run-off into waterways. The effect of estrogen disruptors in fish has mainly been assessed in relation to reproductive endpoints, and relatively little attention has been given to other disruptive actions. This review will overview the actions of estrogens in fish, including ER isoforms, their expression, structure and mechanisms of action. The estrogen functions will be considered in relation to mineral homeostasis and actions on mineralized tissues. The impact of estrogenic endocrine disrupting compounds on fish mineralized tissues will be reviewed, and the potential adverse outcomes of exposure to such compounds will be discussed. Current lacunae in knowledge are highlighted along with future research priorities.

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“…The yeast is transfected with an expression vector encoding an ER and a reporter gene vector composed of an estrogen-sensitive promoter. Reporter gene assays using recombinant yeast cells is a rapid screening alternative to live animal or cell cultures studies (Passos et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

In vitro toxicological assessment of Arrabidaea brachypoda (DC.) Bureau: Mutagenicity and estrogenicity studies

Resende

Nogueira

Espanha

et al. 2017

Regulatory Toxicology and Pharmacology

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Arrabidaea brachypoda (DC.) Bureau is a shrub native Cerrado, known as "cipó-una", "tintureiro" or "cervejinha do campo" and popularly used in Southeastern and Northeastern Brazil to treatment of kidney stones and painful joints (arthritis). Nevertheless, scientific information regarding this species is scarce, and there are no reports related to its possible estrogenic and mutagenic effects. Thus, the principal objective of this study was to assess the mutagenic and estrogenic activities of the hydroalcoholic extracts of the leaves, stalks, roots, their respective fractions and isolated compounds of A. brachypoda. The mutagenic activity was evaluated by the Ames test on Salmonella typhimurium strains TA98, TA97a, TA100 and TA102, in the absence (-S9) and presence (+S9) of metabolic activation system. In the RYA was used Saccharomyces cerevisiae engineered strain BY4741 (MATaura3Δ0 leu2Δ0 his3Δ1 met15Δ0) which reproduce the natural pathway of genetic control by estrogens in vertebrate cells; it has the advantage of its simplicity and a high throughput. All extracts and aqueous fraction of leaves A. brachypoda were mutagenic. The crude extract is more active than the fraction, suggesting a synergic effect. Only hydroalcoholic extracts of leaves and roots of A. brachypoda showed significant estrogenic activity, with ERα-dependent transcriptional activation activity. The obtained results in this study showed the presence of compounds capable of interacting with the estrogen receptor and to induce damage in the genetic material. Thus, we demonstrated the risk which the population is subjected due to indiscriminate use of extracts without detailed study.

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A yeast assay based on the gilthead sea bream (teleost fish) estrogen receptor β for monitoring estrogen mimics

Cited by 12 publications

References 40 publications

Control of Transcriptional Repression of the Vitellogenin Receptor Gene in Largemouth Bass (Micropterus Salmoides) by Select Estrogen Receptors Isotypes

Control of Transcriptional Repression of the Vitellogenin Receptor Gene in Largemouth Bass (Micropterus Salmoides) by Select Estrogen Receptors Isotypes

Effects of Estrogens and Estrogenic Disrupting Compounds on Fish Mineralized Tissues

In vitro toxicological assessment of Arrabidaea brachypoda (DC.) Bureau: Mutagenicity and estrogenicity studies

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