A zinc finger protein gene ZFP5 integrates phytohormone signaling to control root hair development in Arabidopsis

Abstract

). † These authors contributed equally to this work. SUMMARYAlthough root hair development in Arabidopsis thaliana has been extensively studied, it remains unknown whether the zinc finger proteins, the largest family of transcription factors in plants, are involved in this process. Here we report that the C2H2 zinc finger protein ZINC FINGER PROTEIN 5 (ZFP5) is a key regulator of root hair initiation and morphogenesis in Arabidopsis. ZFP5 is mainly expressed in root and preferentially in root hair cells. Using both zfp5 mutants and ZFP5 RNAi lines, we show that reduction in ZFP5 function leads to fewer and much shorter root hairs compared to wild-type. Genetic and molecular experiments demonstrate that ZFP5 exerts its effect on root hair development by directly promoting expression of the CAPRICE (CPC) gene. Furthermore, we show that ZFP5 expression is induced by cytokinin, and that ZFP5 mediates cytokinin and ethylene effects on the formation and growth of root hairs. These results suggest that ZFP5 integrates various plant hormone cues to control root epidermal cell development in Arabidopsis.

OsARF16 Is Involved in Cytokinin-Mediated Inhibition of Phosphate Transport and Phosphate Signaling in Rice (Oryza sativa L.)

“…Cytokinin regulates root system architecture (RSA) by disturbing cell division activity and increasing the length of the root hairs at the root tips [33], [34]. In the present study, we measured the number of lateral roots on the main roots and the average lengths of the root hairs on the root tips of both NIP and the osarf16 mutant under the 0.1 µM 6-BA treatment.…”

Multiple phytohormones promote root hair elongation by regulating a similar set of genes in the root epidermis in Arabidopsis

“…For most experiments, seeds were surface-sterilized for 10 min in 30% bleach solution and rinsed five times with sterile distilled water. After vernalization in the dark for 2 d at 4°C, the seeds were germinated and grown vertically on Murashige and Skoog (MS) plates in a growth chamber, as described previously (An et al , 2012). Growth conditions in the chamber were controlled as follows: 22 ± 2°C, 90–120μmol m –2 s –1 , 16h light/8h dark, 68–78% humidity.…”

“…In addition to auxin and ethylene, our previous research revealed cytokinin as a novel root hair development regulator (An et al , 2012). Application of exogenous cytokinin promotes root hair elongation while lines that overexpress a cytokinin oxidase exhibit a short-hair phenotype (An et al , 2012).…”

“…Application of exogenous cytokinin promotes root hair elongation while lines that overexpress a cytokinin oxidase exhibit a short-hair phenotype (An et al , 2012). The signaling of ethylene and cytokinin is integrated by ZINCFINGERPROTEIN 5 (ZFP5), which is a newly characterized C2H2zinc finger protein (An et al , 2012). …”

The Effects of Fluctuations in the Nutrient Supply on the Expression of Five Members of the AGL17 Clade of MADS-Box Genes in Rice

“…The qPCR was conducted in the following cycling conditions: 95°C for 30 s, followed by 40 cycles of 95°C for 10 s and 60°C for 30 s. Melt curve analysis was used to confirm the absence of non-specific amplification products. Relative expression levels were calculated by subtracting the threshold cycle (Ct) values for OsActin (Os03g0718100) from those of the target gene (to give △Ct) and then calculating 2–△Ct as we described before [40]–[42]. RT-PCR experiments were performed with three biological replicates with the representative being shown.…”