A β-d-glucan isolated from the fruiting bodies of Hericium erinaceus and its aqueous conformation

Dong, Qun; Jia, Lian-meng; Fang, Jing

doi:10.1016/j.carres.2006.01.022

Cited by 68 publications

(27 citation statements)

References 14 publications

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“…The completeness of methylation was confirmed by the disappearance of the hydroxyl absorption in the IR spectrum (Nujol). Methylated alditol acetates were prepared and analyzed by GC–MS [26].…”

Section: Methodsmentioning

confidence: 99%

A RG-II Type Polysaccharide Purified from Aconitum coreanum Alleviates Lipopolysaccharide-Induced Inflammation by Inhibiting the NF-κB Signal Pathway

Jiang

Shi

et al. 2014

PLoS ONE

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Korean mondshood root polysaccharides (KMPS) isolated from the root of Aconitum coreanum (Lévl.) Rapaics have shown anti-inflammatory activity, which is strongly influenced by their chemical structures and chain conformations. However, the mechanisms of the anti-inflammatory effect by these polysaccharides have yet to be elucidated. A RG-II polysaccharide (KMPS-2E, Mw 84.8 kDa) was isolated from KMPS and its chemical structure was characterized by FT-IR and NMR spectroscopy, gas chromatography–mass spectrometry and high-performance liquid chromatography. The backbone of KMPS-2E consisted of units of [→6) -β-D-Galp (1→3)-β-L-Rhap-(1→4)-β-D-GalpA-(1→3)-β-D-Galp-(1→] with the side chain →5)-β-D-Arap (1→3, 5)-β-D-Arap (1→ attached to the backbone through O-4 of (1→3,4)-L-Rhap. T-β-D-Galp is attached to the backbone through O-6 of (1→3,6)-β-D-Galp residues and T-β-D-Ara is connected to the end group of each chain. The anti-inflammatory effects of KMPS-2E and the underlying mechanisms using lipopolysaccharide (LPS) - stimulated RAW 264.7 macrophages and carrageenan-induced hind paw edema were investigated. KMPS-2E (50, 100 and 200 µg/mL) inhibits iNOS, TLR4, phospho-NF-κB–p65 expression, phosphor-IKK, phosphor-IκB-α expression as well as the degradation of IκB-α and the gene expression of inflammatory cytokines (TNF-α, IL-1β, iNOS and IL-6) mediated by the NF-κB signal pathways in macrophages. KMPS-2E also inhibited LPS-induced activation of NF-κB as assayed by electrophorectic mobility shift assay (EMSA) in a dose-dependent manner and it reduced NF-κB DNA binding affinity by 62.1% at 200µg/mL. In rats, KMPS-2E (200 mg/kg) can significantly inhibit carrageenan-induced paw edema as ibuprofen (200 mg/kg) within 3 h after a single oral dose. The results indicate that KMPS-2E is a promising herb-derived drug against acute inflammation.

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Section: Methodsmentioning

confidence: 99%

A RG-II Type Polysaccharide Purified from Aconitum coreanum Alleviates Lipopolysaccharide-Induced Inflammation by Inhibiting the NF-κB Signal Pathway

Jiang

Shi

et al. 2014

PLoS ONE

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“…In this field, a β-(1→3)-linked D-glucan with single unit glucosyl branches attached at O-6 of every third backbone residue has been isolated from Hericium erinaceus [51]. Heteropolysaccharides have been also isolated from this mushroom; thus, an α-(1→6)-linked D-galactopyranosyl with rhamnose and glucose branches at O-2 has been described [52].…”

Section: Structural Features Of Polysaccharides Occurring In Mushroomsmentioning

confidence: 99%

Structural Features and Healthy Properties of Polysaccharides Occurring in Mushrooms

2012

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Abstract:Polysaccharides from mushrooms have attracted a great deal of attention due to the many healthy benefits they have demonstrated, such as immunomodulation, anticancer activity, prevention and treatment of cardiovascular diseases, antiviral and antimicrobial effects, among others. Isolation and purification of polysaccharides commonly involve several steps, and different techniques are actually available in order to increase extraction yield and purity. Studies have demonstrated that the molecular structure and arrangement significantly influence the biological activity; therefore, there is a wide range of analytical techniques for the elucidation of chemical structures. Different polysaccharides have been isolated from mushrooms, most of them consisting of β-linked glucans, such as lentinan from Lentinus edodes, pleuran from Pleurotus species, schizophyllan from Schizophyllum commune, calocyban from Calocybe indica, or ganoderan and ganopoly from Ganoderma lucidum. This article reviews the main methods of polysaccharide isolation and structural characterization, as well as some of the most important polysaccharides isolated from mushrooms and the healthy benefits they provide.

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“…This mushroom is called Yamabushitake in Japan, and has β glucan and other polysaccharides as components of the cell wall like many other basidiomycetes. 19,20) Recently, Yamabushitake became commercially available in Nagano Prefecture, Japan. However, the chemical properties of this mushroom have not been fully investigated.…”

mentioning

confidence: 99%

Optimization of Microwave-assisted Extraction of Polysaccharides from the Fruiting Body of Mushrooms

大櫛¹,

坂本²,

東³

2006

J. Appl. Glycosci.

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Microwave irradiation in the presence of water was applied for extraction of polysaccharides from the fruiting body of Hericium erinaceum, a mushroom called Yamabushitake. In this study, the effects of microwave irradiation temperature and time on solubilization of polymers in H. erinaceum were investigated. The results indicated that the degree of solubilization of polymers proceeded with an increase in heating temperature up to 200 C, although the polymers solubilized gradually depolymerized with the increase in heating temperature. The results also suggested that lengthening irradiation time had effects on solubilization of polymers similar to the increase in heating temperature. Comparison of the results obtained by microwaveassisted extraction with those obtained by extraction using conventional external heating indicated that extractability of the former extraction for 5 min at 140 C was almost equivalent to that of the latter extraction for 6 h at 100 C. This result suggests that microwave irradiation has an advantage for extraction of polysaccharides from the fruiting body of mushrooms in terms of extraction time.

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A β-d-glucan isolated from the fruiting bodies of Hericium erinaceus and its aqueous conformation

Cited by 68 publications

References 14 publications

A RG-II Type Polysaccharide Purified from Aconitum coreanum Alleviates Lipopolysaccharide-Induced Inflammation by Inhibiting the NF-κB Signal Pathway

A RG-II Type Polysaccharide Purified from Aconitum coreanum Alleviates Lipopolysaccharide-Induced Inflammation by Inhibiting the NF-κB Signal Pathway

Structural Features and Healthy Properties of Polysaccharides Occurring in Mushrooms

Optimization of Microwave-assisted Extraction of Polysaccharides from the Fruiting Body of Mushrooms

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