ABA and GA4 dynamic modulates secondary dormancy and germination in Syngonanthus verticillatus seeds

Tong

et al. 2022

JoF

Sanghuangporus baumii is a traditional medicinal fungus that produces pharmacological terpenoids, but natural resources are insufficient for applications, and its growth and development mechanisms are poorly understood. Combining metabolomic and transcriptomic analyses, we found four terpenoid hormones and a central gene, isopentenyl diphosphate isomerase (IDI), involved in growth and development. Additionally, an exogenous hormone test was used to further confirm the importance of the four terpenoid hormones. Finally, hormone content determination and qRT−PCR were performed to explore the growth and development mechanism; we found thatcis-zeatin (CZ) plays a major role in the mycelia stage, trans-zeatin (TZ) and gibberellin A4 (GA4) are important in the primordia stage, GA4 is crucial for the fruiting bodies stage, and abscisic acid (ABA) may be a marker of maturity. The IDI gene was also found to affectterpenoid hormone content by regulating the relative gene transcript levels, thereby controlling morphological changes in S. baumii. Our results revealthe growth and development mechanisms of S. baumii and may promote the breeding and utilisation of high-quality varieties.

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Metabolome and Transcriptome Profiling Reveal That Four Terpenoid Hormones Dominate the Growth and Development of Sanghuangporus baumii

Tong

et al. 2022

JoF

“…Overexpression of the IDI gene does not increase the contents of all terpenoid hormones, mainly due to competition among hormones and tissue-specific expression (Barreto, Herken, Silva, MunneBosch, & Garcia, 2020;Zhang et al, 2014). IT S. baumii CZ, TZ and GA4 levels and related genes were decreased in Myc and Pri stages, which was probably caused by an increase in triterpenoids content caused by high-level expression of triterpenoids biosynthetic genes (Figure 7).…”

Section: Effects Of Four Exogenous Terpenoid Hormones On the Growth And Development Of Wt And It S Baumii Strainsmentioning

confidence: 99%

Metabolome and transcriptome profiling reveals that terpenoid biosynthesis dominates the growth and development of Sanghuangporus baumii

Wang

et al. 2022

Preprint

Sanghuangporus baumii is a traditional medicinal fungus that produces pharmacological terpenoids, but natural resources are insufficient for applications, and growth and development mechanisms are poorly understood. Combining metabolomic and transcriptomic analyses, we identified four terpenoid hormones and a central gene isopentenyl diphosphate isomerase (IDI) involved in growth and development. The importance of the four terpenoid hormones was further confirmed by exogenous hormones addition, we found that Cis-Zeatin (CZ) plays a major role in the mycelia stage, Trans-Zeatin (TZ) and Gibberellin A4 (GA4) are important in the primordia stage, GA4 is crucial for the fruiting bodies stage, and Abscisic Acid (ABA) may be a marker of maturity. Later, hormone content determination and qRT-PCR were used to verify the growth and development mechanism, and finally it was confirmed that IDI gene affects the content of terpenoid hormones content by regulating the transcript level of terpenoid biosynthesis genes, thus affecting the morphological changes of S. baumii. Our results illuminate the growth and development mechanisms of S. baumii, and may promote the breeding and utilisation of high-quality varieties.

“…Like indispensable lubrication in plants lifecycle, various plant hormones regulate plant growth and development synergistically or antagonistically, their relationship woven into a series of complex networks in plants [32,[52][53][54]. Abscisic acid (ABA) and GA work antagonistically in regulating various plant growth and development events such as seed dormancy and germination, root growth, plant height, flowering initiation, and abiotic stress [11,55,56].…”

Section: Osabf1 May Mediate the Antagonistic Relationship Of Aba And Gamentioning

confidence: 99%

OsABF1 Represses Gibberellin Biosynthesis to Regulate Plant Height and Seed Germination in Rice (Oryza sativa L.)

Tang

Wang

et al. 2021

IJMS

Gibberellins (GAs) are diterpenoid phytohormones regulating various aspects of plant growth and development, such as internode elongation and seed germination. Although the GA biosynthesis pathways have been identified, the transcriptional regulatory network of GA homeostasis still remains elusive. Here, we report the functional characterization of a GA-inducible OsABF1 in GA biosynthesis underpinning plant height and seed germination. Overexpression of OsABF1 produced a typical GA-deficient phenotype with semi-dwarf and retarded seed germination. Meanwhile, the phenotypes could be rescued by exogenous GA3, suggesting that OsABF1 is a key regulator of GA homeostasis. OsABF1 could directly suppress the transcription of green revolution gene SD1, thus reducing the endogenous GA level in rice. Moreover, OsABF1 interacts with and transcriptionally antagonizes to the polycomb repression complex component OsEMF2b, whose mutant showed as similar but more severe phenotype to OsABF1 overexpression lines. It is suggested that OsABF1 recruits RRC2-mediated H3K27me3 deposition on the SD1 promoter, thus epigenetically silencing SD1 to maintain the GA homeostasis for growth and seed germination. These findings shed new insight into the functions of OsABF1 and regulatory mechanism underlying GA homeostasis in rice.