ABC Spotlight on paper-based strips analytics

Gauglitz, Günter

doi:10.1007/s00216-017-0707-x

Cited by 14 publications

(8 citation statements)

References 10 publications

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“…The results showed that an optimal separation with the best sensitivity was obtained with water/methanol/acetic acid (94/5/1, v / v / v (A)) and methanol/water/acetic acid (97/2/1, v / v / v (B)), both adjusted with 5 mM ammonium acetate. These results are in accordance with previously reported LC-MS/MS methods for multi-mycotoxin analysis [ 40 ].…”

Section: Resultssupporting

confidence: 93%

“…The two most intense product ions were selected for the MRM transitions of the MS method after applying different collision energies ( Table 1 ). Based on previous studies carried out in the same laboratory [ 24 , 39 , 40 ], an Acquity UPLC ® HSS T3 [1.8 µm, 2.1 mm × 100 mm] chromatographic column was chosen, demonstrating proper retention times for all the analyzed mycotoxins. Different mobile phases for an optimal separation, including mixtures of water with a volatile organic acid or ammonium acetate with an organic solvent (methanol) were tested [ 24 , 41 ].…”

Section: Resultsmentioning

confidence: 99%

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Volumetric Absorptive Microsampling as an Alternative Tool for Biomonitoring of Multi-Mycotoxin Exposure in Resource-Limited Areas

Vidal

Belova

Stove

et al. 2021

Toxins

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Biomonitoring of biological samples arises as an effective tool to evaluate the exposure to mycotoxins in the population. Owing to the wide range of advantages, there is a growing interest in the use of non- and minimally invasive alternative sampling strategies, such as dried blood spot sampling or volumetric absorptive microsampling (VAMS). A VAMS-based multi-mycotoxin method was developed and validated for 24 different mycotoxins. Method validation was based on the Bioanalytical Method Validation Guideline of the Food and Drug Administration from the United States and for most of the studied mycotoxins, the results of the performance characteristics were in agreement with the criteria of the European Commission Decision 2002/657/EC. The recovery for the different mycotoxins was not haematocrit dependent and remained acceptable after storing the VAMS for 7 and 21 days at refrigeration temperature (4 °C) and room temperature, demonstrating that VAMS could be applied to assess mycotoxin exposure in blood in resource-limited areas, where there may be a delay between sampling and analysis. Finally, a comparison between VAMS and a procedure for liquid whole blood analysis, performed on 20 different blood samples, did not result in missed exposed cases for VAMS. Moreover, both methods detected similar levels of ochratoxin A, ochratoxin alpha, zearalenone and aflatoxin B1. Given all the benefits associated with VAMS and the developed method, VAMS sampling may serve as an alternative to conventional venous sampling to evaluate multiple mycotoxin exposure.

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Section: Resultssupporting

confidence: 93%

Section: Resultsmentioning

confidence: 99%

Volumetric Absorptive Microsampling as an Alternative Tool for Biomonitoring of Multi-Mycotoxin Exposure in Resource-Limited Areas

Vidal

Belova

Stove

et al. 2021

Toxins

View full text Add to dashboard Cite

show abstract

“…The materials used were gelatin as extracellular matrix and titanium oxide and silver nanoparticles. The device can measure in vitro contractile effects by cardiotoxicity of nanoparticles that affect calcium signal to sarcomere [ 108 ]. Cardiomyocytes clusters in spheroid geometry are part of an experiment for drug testing in a microfluidic device.…”

Section: Microfluidic: Organ-on-a-chipmentioning

confidence: 99%

Organs-on-a-Chip Module: A Review from the Development and Applications Perspective

et al. 2018

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In recent years, ever-increasing scientific knowledge and modern high-tech advancements in micro- and nano-scales fabrication technologies have impacted significantly on various scientific fields. A micro-level approach so-called “microfluidic technology” has rapidly evolved as a powerful tool for numerous applications with special reference to bioengineering and biomedical engineering research. Therefore, a transformative effect has been felt, for instance, in biological sample handling, analyte sensing cell-based assay, tissue engineering, molecular diagnostics, and drug screening, etc. Besides such huge multi-functional potentialities, microfluidic technology also offers the opportunity to mimic different organs to address the complexity of animal-based testing models effectively. The combination of fluid physics along with three-dimensional (3-D) cell compartmentalization has sustained popularity as organ-on-a-chip. In this context, simple humanoid model systems which are important for a wide range of research fields rely on the development of a microfluidic system. The basic idea is to provide an artificial testing subject that resembles the human body in every aspect. For instance, drug testing in the pharma industry is crucial to assure proper function. Development of microfluidic-based technology bridges the gap between in vitro and in vivo models offering new approaches to research in medicine, biology, and pharmacology, among others. This is also because microfluidic-based 3-D niche has enormous potential to accommodate cells/tissues to create a physiologically relevant environment, thus, bridge/fill in the gap between extensively studied animal models and human-based clinical trials. This review highlights principles, fabrication techniques, and recent progress of organs-on-chip research. Herein, we also point out some opportunities for microfluidic technology in the future research which is still infancy to accurately design, address and mimic the in vivo niche.

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“…Concerning the reaction format, the single-step synthetic route was applied, which was not only simple and fast but also generated super-porosity for the selectivity of monolith [23]. Therefore, the optimization factors of the ratio of the template/functional monomer/cross-linker, the reaction time and the reaction temperature were investigated in the preparation of the molecularly imprinted monolith [24,25].…”

Section: Preparation Of the Molecularly Imprinted Monolithmentioning

confidence: 99%

Determination of sulfamerazine in aquatic products by molecularly imprinted capillary electrochromatography

Qin

Jin²,

Gao

et al. 2019

R. Soc. open sci.

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A molecularly imprinted monolith was prepared and evaluated for the special selective separation of sulfamerazine (SMR) by capillary electrochromatography (CEC). The single-step in situ polymerization method was applied through thermally immobilized vinyl groups of itaconic acid and a derivatization capillary column using SMR as the template. The monolith with optimal selectivity and permeability was performed at 45°C for 7 h according to the molar ratios of 1 : 4 : 10 (template/functional monomer/cross-linker). Under the optimized separation conditions of 75% acetonitrile in 20 mM phosphate buffer with pH 5.0, 15 kV applied voltage and 20°C column temperature, the imprinted monolith showed strong recognition ability for SMR and high column performance. Finally, the molecularly imprinted monolith coupled with the CEC method was successfully developed for the quantification of SMR in aquatic products, which was properly validated by a good linear relationship, recoveries and limit of detection. The coupling technique of the molecularly imprinted technology and CEC achieved pre-treatment enrichment and separation analysis in only one miniaturized chromatographic column.

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ABC Spotlight on paper-based strips analytics

Cited by 14 publications

References 10 publications

Volumetric Absorptive Microsampling as an Alternative Tool for Biomonitoring of Multi-Mycotoxin Exposure in Resource-Limited Areas

Volumetric Absorptive Microsampling as an Alternative Tool for Biomonitoring of Multi-Mycotoxin Exposure in Resource-Limited Areas

Organs-on-a-Chip Module: A Review from the Development and Applications Perspective

Determination of sulfamerazine in aquatic products by molecularly imprinted capillary electrochromatography

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