Abce1 orchestrates M-phase entry and cytoskeleton architecture in mouse oocyte

Jiao, Xiangmin; Huang, Chun-Jie; Wu, Di; Zhang, Jiayu; Long, Yuting; Chen, Fan; Li, Xiang; Huo, Li‐Jun

doi:10.18632/oncotarget.16546

Cited by 19 publications

(8 citation statements)

References 30 publications

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“…Defects in spindle assembly and abnormality of chromosome alignments can result in the failure of PBE. (Dumont and Desai 2012;Huang et al 2017;Jiao et al 2017). More importantly, studies have shown that cellular ATP level is a vital event to ensure the normal bipolar spindle assembly and the decrease of ATP content results in various spindle defects and chromosome alignment anomaly (Eichenlaub-Ritter et al 2004;Zhang et al 2006).…”

Section: Discussionmentioning

confidence: 99%

Effects of Acute Fluorene‐9‐Bisphenol Exposure on Mouse Oocyte in vitro Maturation and Its Possible Mechanisms

Jiao

Liang

et al. 2018

Environ and Mol Mutagen

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Fluorene‐9‐bisphenol (BHPF), a substitute of bisphenol A (BPA) used in the production of the so‐called “BPA‐free” plastics, has now been shown to be released from commercial plastic bottles into drinking water and has strong anti‐estrogenic activity in mice, which suggests that BHPF is also an environmental toxin. However, whether BHPF exposure has effects on mouse oocyte development is unknown. In this study, the influence of acute exposure to BHPF (50–150 μM, 12 hr) on mouse oocyte maturation and its possible mechanisms were investigated. Of note, 50‐μM BHPF had no effects on the maturation of mouse oocytes, whereas 100‐ and 150‐μM BHPF significantly blocked germinal vesicle breakdown and led to the failure of first polar body extrusion. Particularly, 100‐μM BHPF exposure severely decreased the cellular adenosine triphosphate in a time‐dependent manner, which finally brought out the loss of spindles. In addition, the actin cytoskeleton was also impaired. The defective mitochondrial dynamics and decreased mitochondrial DNA implied the damage of mitochondria in BHPF‐treated oocytes. Increased PINK1, Beclin1, and LC3B protein level and decreased TOMM20 and TOMM17A protein level illustrated that mitophagy was induced, which also confirmed that BHPF exposure impaired the cellular mitochondria. Moreover, BHPF induced reactive oxygen species accumulation and early apoptosis. Oocyte quality was also impaired by BHPF exposure through altering histone modifications evidenced by increased H3K9me3 and H3K27me3 levels. Collectively, our results indicated that BHPF exposure disrupted mouse oocyte maturation and reduced oocyte quality through affecting cytoskeleton architecture, mitochondrial function, oxidative stress, apoptosis, and histone modifications. Environ. Mol. Mutagen. 60:243–253, 2019. © 2018 Wiley Periodicals, Inc.

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Section: Discussionmentioning

confidence: 99%

Effects of Acute Fluorene‐9‐Bisphenol Exposure on Mouse Oocyte in vitro Maturation and Its Possible Mechanisms

Jiao

Liang

et al. 2018

Environ and Mol Mutagen

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“…One of the hallmarks of the metastatic process is altered cellular motility [12], a complex process requiring dramatic remodeling of the cell cytoskeleton. Because accumulating evidence indicates that ABCE1 plays an important role in cytoskeleton regulation [13–15], PubMed curation was used to filter the remaining eleven genes (excluding ABCE1) by their association with cytoskeleton organization. We found that LCP1 is an actin binding protein that participates in cytoskeleton remodeling [16–18].…”

Section: Resultsmentioning

confidence: 99%

Dual inhibition of ABCE1 and LCP1 by microRNA-96 results in an additive effect in breast cancer mouse model

2019

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MicroRNAs (miRNAs) are short non-coding RNAs that regulate the expression of target genes at the post-transcriptional level. Each miRNA can modulate multiple genes and, as a result, a single miRNA may have a profound effect on a specific biological pathway consisting of several of its target genes. Recent studies have indicated that specific miRNA signatures are correlated with tumor aggressiveness and clinical outcome in breast cancer. We previously demonstrated that miR-96 has a suppressive effect on breast cancer aggressiveness and that this effect was mediated by ABCE1 gene regulation. In this study we investigated whether other miR-96 regulated genes can enhance ABCE1’s anti-cancer effects. We identified one such gene – LCP1 – and proved its negative effect on breast cancer progression. Interestingly, dual inhibition of ABCE1 and LCP1 resulted in an additive effect on cancer cell migration, invasion, and proliferation. Furthermore, in vivo analysis of dual ABCE1 and LCP1 knockdown resulted in significant tumor growth inhibition, decreased metastatic activity, and contributed to survival compared to either gene, separately. This indicates that the combined downregulation of two miR-96 gene targets has an additive effect on reducing cancer aggressiveness. Overall, our work supports seeking more than one target in miRNA-based studies in order to enhance functional effects and better characterize the miRNA wide-spread activity.

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“…Spindle assembly and chromosome segregation are two indispensable events during the progress of meiosis. Defective morphology of spindles and abnormal chromosome alignments could result in aneuploid gametes or the failure of meiosis (Ma et al, 2014;Jiao et al, 2017;Han et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…Spindle assembly and chromosome segregation are both vital courses to keep genomic stability during oocyte meiosis. Errors in these two processes can lead to the failure of meiosis or the generation of aneuploidy (Ma et al, 2014;Jiao et al, 2017;Han et al, 2015). Moreover, human pregnancy loss has strong correlation with fetal aneuploidy which can be induced by defective spindle structure and abnormal chromosome segregation (Webster et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Peer Review #1 of "OLA1 is responsible for normal spindle assembly and SAC activation in mouse oocytes (v0.1)"

2020

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Background. OLA1 is a member of the GTPase protein family, unlike other members, it possess both GTPase and ATPase activities, and can bind and hydrolyze ATP more efficiently than GTP. OLA1 participates in cell proliferation, oxidative response, protein synthesis and tumorigenesis. However, whether OLA1 is also required for oocyte meiosis is still unknown. Methods. In this study, the localization, expression, and functions of OLA1 in the mouse oocyte meiosis were examined. Immunofluorescent and confocal microscopy were used to explore the location pattern of OLA1 in the mouse oocyte. Moreover, nocodazole treatment was used to confirm the spindle-like location of OLA1 during mouse meiosis. Western blot was used to explore the expression pattern of OLA1 in the mouse oocyte. Microinjection of siRNA was used to explore the OLA1 functions in the mouse oocyte meiosis. In addition, chromosome spreading was used to investigate the spindle assembly checkpoint (SAC) activity. Results. Immunofluorescent staining showed that OLA1 evenly distributed in the cytoplasm at germinal vesicle (GV) stage. After meiosis resumption (GVBD), OLA1 co-localized with spindles, which was further identified by nocodazole treatment experiments. Knockdown of OLA1 impaired the germinal vesicle breakdown progression and finally resulted in a lower polar body extrusion rate. Immunofluorescence analysis indicated that knockdown of OLA1 led to abnormal spindle assembly, which was evidenced by multipolar spindles in OLA1-RNAi-oocytes. After 6 h post-GVBD in culture, an increased proportion of oocyte which has precociously entered into anaphase/telephase I (A/TI) was observed in OLA1knockdown oocytes, suggesting that loss of OLA1 resulted in the premature segregation of homologous chromosomes. In addition, the chromosome spread analysis suggested that OLA1 knockdown induced premature anaphase onset was due to the precocious inactivation of SAC. Taken together, we concluded that OLA1 plays important role in GVBD, spindle assembly and SAC activation maintenance in oocyte meiosis.

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Abce1 orchestrates M-phase entry and cytoskeleton architecture in mouse oocyte

Cited by 19 publications

References 30 publications

Effects of Acute Fluorene‐9‐Bisphenol Exposure on Mouse Oocyte in vitro Maturation and Its Possible Mechanisms

Effects of Acute Fluorene‐9‐Bisphenol Exposure on Mouse Oocyte in vitro Maturation and Its Possible Mechanisms

Dual inhibition of ABCE1 and LCP1 by microRNA-96 results in an additive effect in breast cancer mouse model

Peer Review #1 of "OLA1 is responsible for normal spindle assembly and SAC activation in mouse oocytes (v0.1)"

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