ABCE1 plays an essential role in lung cancer progression and metastasis

Tian, Ye; Tian, Xin; Han, Xu; Chen, Yong; Song, Cheng‐Yang; Jiang, Wenjun; Tian, Dean

doi:10.1007/s13277-015-4713-3

Cited by 33 publications

(26 citation statements)

References 24 publications

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“…Rapid growth and increased metastatic potential are two basic characteristics of tumor cells. Recent evidence has indicated that ABCE1 is crucial in tumor progression, prognosis and metastasis [7, 9]. Our MTT and clonogenic assay data showed that A549 cells overexpressing ABCE1 grew much faster and formed more pronounced and a larger number of colonies.…”

Section: Discussionmentioning

confidence: 53%

“…Although an increasing number of studies has demonstrated the role of ABCE1 in tumor cell development, very little is known about the actual mechanisms [7, 25]. Furthermore, the roles of ABCE1 and its Fe-S clusters in carcinogenesis are still undetermined.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, emerging studies have reported increased ABCE1 expression in human tumors [7, 8]. Furthermore, the levels of ABCE1 have been closely linked to clinical stages, which indicates that elevated ABCE1 expression may induce tumor proliferation and promote invasive behavior [9].…”

Section: Introductionmentioning

confidence: 99%

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Deficiency of Functional Iron-Sulfur Domains in ABCE1 Inhibits the Proliferation and Migration of Lung Adenocarcinomas By Regulating the Biogenesis of Beta-Actin In Vitro

Han

Tian³

2017

Cell Physiol Biochem

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Background/Aims: ATP-binding cassette transporter E1 (ABCE1), a unique ABC superfamily member that bears two Fe-S clusters, is essential for metastatic progression in lung cancer. Fe-S clusters within ABCE1 are crucial for ribosome dissociation and translation reinitiation; however, whether these clusters promote tumor proliferation and migration is unclear. Methods: The interaction between ABCE1 and β-actin was confirmed using GST pull-down. The lung adenocarcinoma (LUAD) cell line A549 was transduced with lentiviral packaging vectors overexpressing either wild-type ABCE1 or ABCE1 with Fe-S cluster deletions (ΔABCE1). The role of Fe-S clusters in the viability and migration of cancer cells was evaluated using clonogenic, MTT, Transwell and wound healing assays. Cytoskeletal rearrangement was determined using immunofluorescent techniques. Results: Fe-S clusters were the key domains in ABCE1 involved in binding to β-actin. The proliferative and migratory capacity increased in cells overexpressing ABCE1. However, the absence of Fe-S clusters reversed these effects. A549 cells overexpressing ABCE1 exhibited irregular morphology and increased levels of cytoskeletal polymerization as indicated by the immunofluorescence images. In contrast, cells expressing the Fe-S cluster deletion mutant presented opposing effects. Conclusion: These results demonstrate the indispensable role of Fe-S clusters when ABCE1 participates in the proliferation and migration of LUADs by interacting with β-actin. The Fe-S clusters of ABCE1 may be potential targets for the prevention of lung cancer metastasis.

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Section: Discussionmentioning

confidence: 53%

Section: Discussionmentioning

confidence: 99%

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Deficiency of Functional Iron-Sulfur Domains in ABCE1 Inhibits the Proliferation and Migration of Lung Adenocarcinomas By Regulating the Biogenesis of Beta-Actin In Vitro

Han

Tian³

2017

Cell Physiol Biochem

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“…Our study showed that CXCL1 is implicated in GC overall survival. ABCE1 (ATP-binding cassette E1) plays a crucial role in the metastasis and progress of lung cancers, and therefore it has been suggested as a valuable therapeutic target for the management of these cancers [44]. We have demonstrated in this study that ABCE1 is elevated in GC and is an indicator for the prognosis of GC.…”

Section: Discussionmentioning

confidence: 57%

A Novel Gene Expression-Based Prognostic Scoring System to Predict Survival in Gastric Cancer

Wang¹

2017

Gastroenterology

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Analysis of gene expression patterns in gastric cancer (GC) can help to identify a comprehensive panel of gene biomarkers for predicting clinical outcomes and to discover potential new therapeutic targets. Here, a multi-step bioinformatics analytic approach was developed to establish a novel prognostic scoring system for GC. We first identified 276 genes that were robustly differentially expressed between normal and GC tissues, of which, 249 were found to be significantly associated with overall survival (OS) by univariate Cox regression analysis. The biological functions of 249 genes are related to cell cycle, RNA/ncRNA process, acetylation and extracellular matrix organization. A network was generated for view of the gene expression architecture of 249 genes in 265 GCs. Finally, we applied a canonical discriminant analysis approach to identify a 53-gene signature and a prognostic scoring system was established based on a canonical discriminant function of 53 genes. The prognostic scores strongly predicted patients with GC to have either a poor or good OS. Our study raises the prospect that the practicality of GC patient prognosis can be assessed by this prognostic scoring system.

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“…The study of Wang showed that CXCL1 is also involved in gastric cancer overall survival. ATPbinding cassete E1 (ABCE1) known to play a crucial role in the metastasis of lung cancers, and therefore, a potential therapeutic target in this seting [70], was also elevated in gastric tumors and predicted the prognosis of patients. …”

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confidence: 99%

Molecular Prognostic Factors in Gastric Cancer

Lazăr¹,

Tăban²,

Cornianu³

et al. 2017

Gastric Cancer

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Gastric cancer represents a major health problem worldwide. Literature data have demonstrated that gastric tumors present a high molecular heterogeneity, responsible for the process of carcinogenesis and dissemination. By revealing the molecular subtype of the tumor, it is possible to assess its behavior, the outcome of the patient, and the treatment approach, according to its genetic and epigenetic proile. This chapter aims to highlight some of the many diferent genetic mutations, epigenetic alterations, as well as aberrant signaling pathways involved in the pathogenesis of stomach cancers, each of these molecular abnormalities acting in a speciic stage of the disease. Moreover, the manuscript describes the novel therapeutic agents that target some of these aberrant molecular signaling pathways. Unfortunately, only a few agents are currently part of the standard treatment of gastric cancer, while most of the others remain to prove their therapeutic eicacy in the seting of clinical trials. By discovering the diferent molecular subtypes of gastric cancer, as well as numerous classes of targeted molecular agents, in the future, we would be able to perform an individualized treatment, associated with maximum eiciency and less costs.Keywords: gastric cancer, molecular classiication, gene expressions-based prognostic scoring system, molecular biomarkers, molecular targeted treatment IntroductionDespite a decline in the incidence in past decades, gastric cancer remains a major health problem globally [1,2], being the ifth most common type of cancer worldwide, with almost one million new cases estimated to have occurred in 2012, according to Globocan [3]. Furthermore, stomach cancer represents the third leading cause of cancer death in both sexes worldwide (723,000 deaths) [3].© 2017 The Author(s). Licensee InTech. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.According to the World Health Organization classiication, the vast majority of gastric cancers are adenocarcinomas, divided into papillary, tubular, mucinous (colloid), and poorly cohesive carcinomas (including signet-ring cell carcinoma and other variants) [4]. Although it was proposed a long time ago (1965), the Lauren classiication is still widely used in clinical practice and subdivides gastric carcinomas into intestinal and difuse types, associated with diferent pathogenesis, ways of spreading, and outcome [5]. Unfortunately, these two classiication systems have litle clinical impact, making the development of classiiers that can deine prognosis and guide patient's treatment as an urgent need.Literature data have demonstrated that the development of gastric cancer is associated in the majority of cases with infectious agents such as the Gram-negative spiral bacterium Helicobacter pylori (most often) [6] and Epstein-Barr virus (EBV) (about 9% o...

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ABCE1 plays an essential role in lung cancer progression and metastasis

Cited by 33 publications

References 24 publications

Deficiency of Functional Iron-Sulfur Domains in ABCE1 Inhibits the Proliferation and Migration of Lung Adenocarcinomas By Regulating the Biogenesis of Beta-Actin In Vitro

Deficiency of Functional Iron-Sulfur Domains in ABCE1 Inhibits the Proliferation and Migration of Lung Adenocarcinomas By Regulating the Biogenesis of Beta-Actin In Vitro

A Novel Gene Expression-Based Prognostic Scoring System to Predict Survival in Gastric Cancer

Molecular Prognostic Factors in Gastric Cancer

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