ABCG transporters export cutin precursors for the formation of the plant cuticle

Elejalde-Palmett, Carolina; Segundo, Ignacio Martinez San; Garroum, Imène; Charrier, Laurence; Bellis, Damien De; Mucciolo, Antonio; Guerault, Aurore; Liu, Jie; Zeisler‐Diehl, Viktoria; Aharoni, Asaph; Schreiber, Lukas; Bakan, Bénédicte; Clausen, Mads Hartvig; Geisler, Markus; Nawrath, Christiane

doi:10.1016/j.cub.2021.02.056

Cited by 38 publications

(24 citation statements)

References 72 publications

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“…Under low temperatures, four LTPs and three LTPs were up-or downregulated, respectively, which might result in stable cuticular wax secretion. Accumulation of evidence indicated that ABCGs [ATP-binding cassette (ABC) subfamily G members] functioned to plant surface protecting against environmental stresses, and most of the reported members are involved in cutin formation (Elejalde-Palmett et al, 2021;Lee et al, 2021). In banana fruit, four of the five differential ABCG11 members were downregulated.…”

Section: Discussionmentioning

confidence: 99%

Changes of Morphology, Chemical Compositions, and the Biosynthesis Regulations of Cuticle in Response to Chilling Injury of Banana Fruit During Storage

et al. 2021

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The plant cuticle covers almost all the outermost surface of aerial plant organs, which play a primary function in limiting water loss and responding to the environmental interactions. Banana fruit is susceptible to thermal changes with chilling injury below 13°C and green ripening over 25°C. Herein, the changes of surface morphology, chemical compositions of cuticle, and the relative expression of cuticle biosynthesis genes in banana fruit under low-temperature storage were investigated. Banana fruit exhibited chilling injury rapidly with browned peel appearance stored at 4°C for 6 days. The surface altered apparently from the clear plateau with micro-crystals to smooth appearance. As compared to normal ones, the overall coverage of the main cuticle pattern of waxes and cutin monomers increased about 22% and 35%, respectively, in browned banana stored under low temperature at 6 days. Fatty acids (C16–C18) and ω-OH, mid-chain-epoxy fatty acids (C18) dominated cutin monomers. The monomers of fatty acids, the low abundant ω, mid-chain-diOH fatty acids, and 2-hydroxy fatty acids increased remarkably under low temperature. The cuticular waxes were dominated by fatty acids (> C19), n-alkanes, and triterpenoids; and the fatty acids and aldehydes were shifted to increase accompanied by the chilling injury. Furthermore, RNA-seq highlighted 111 cuticle-related genes involved in fatty acid elongation, biosynthesis of very-long-chain (VLC) aliphatics, triterpenoids, and cutin monomers, and lipid-transfer proteins were significantly differentially regulated by low temperature in banana. Results obtained indicate that the cuticle covering on the fruit surface was also involved to respond to the chilling injury of banana fruit after harvest. These findings provide useful insights to link the cuticle on the basis of morphology, chemical composition changes, and their biosynthesis regulations in response to the thermal stress of fruit during storage.

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Section: Discussionmentioning

confidence: 99%

Changes of Morphology, Chemical Compositions, and the Biosynthesis Regulations of Cuticle in Response to Chilling Injury of Banana Fruit During Storage

et al. 2021

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“…ABCG11 likely transports cutin precursors as a homodimer that forms with high affinity (Bird et al, 2007). The heterologous expression of AtABCG11 in protoplasts of Nicotiana benthamiana was observed to lead to the export of free and glycerol-bound hydroxylated fatty acids, consistent with a role in cutin precursor export (Elejalde-Palmett et al, 2021). Nevertheless, the possibility of heterodimerization with other ABCG half-transporters cannot be excluded.…”

Section: Abcg Half-transporters Are Required For Cuticle Formationmentioning

confidence: 90%

“…Although AtABCG32 homologs have been tentatively identified in a few non-vascular plant lineages, it appears that at least one homolog is present in all seed plants ( Philippe et al, 2020b ). A duplication of AtABCG32 has been identified in members of the Solanaceae ( Elejalde-Palmett et al, 2021 ); however, whether the two paralogs have evolved different substrate specificities is still an open question. In the tobacco protoplast system, ABCG32 homologs transport oxygenated cutin precursors without selectivity for the structure at the terminal carbons or the mid-chain position, raising the possibility that these proteins may also transport yet uncharacterized cutin precursors ( Elejalde-Palmett et al, 2021 ).…”

Section: Export Of Cuticular Components By Abc Transportersmentioning

confidence: 99%

Trafficking Processes and Secretion Pathways Underlying the Formation of Plant Cuticles

et al. 2022

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Cuticles are specialized cell wall structures that form at the surface of terrestrial plant organs. They are largely comprised lipidic compounds and are deposited in the apoplast, external to the polysaccharide-rich primary wall, creating a barrier to diffusion of water and solutes, as well as to environmental factors. The predominant cuticle component is cutin, a polyester that is assembled as a complex matrix, within and on the surface of which aliphatic and aromatic wax molecules accumulate, further modifying its properties. To reach the point of cuticle assembly the different acyl lipid-containing components are first exported from the cell across the plasma membrane and then traffic across the polysaccharide wall. The export of cutin precursors and waxes from the cell is known to involve plasma membrane-localized ATP-binding cassette (ABC) transporters; however, other secretion mechanisms may also contribute. Indeed, extracellular vesiculo-tubular structures have recently been reported in Arabidopsis thaliana (Arabidopsis) to be associated with the deposition of suberin, a polyester that is structurally closely related to cutin. Intriguingly, similar membranous structures have been observed in leaves and petals of Arabidopsis, although in lower numbers, but no close association with cutin formation has been identified. The possibility of multiple export mechanisms for cuticular components acting in parallel will be discussed, together with proposals for how cuticle precursors may traverse the polysaccharide cell wall before their assimilation into the cuticle macromolecular architecture.

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“…Direct measurement of the transport of hydrophobic molecules is technically very difficult and requires non-conventional approaches ( Lefevre and Boutry, 2018 ). A recent report, showing ABC-mediated sn2-MAG transport, may set a path for establishing the biochemical action of STR/STR2 ( Elejalde-Palmett et al, 2021 ). Given the central role of AM fungal lipid auxotrophy in explaining the obligate nature of the symbiosis, it is critical to fully understand how lipids are transferred in the PAM.…”

Section: Arbuscule Nutrient Exchangementioning

confidence: 99%

A roadmap of plant membrane transporters in arbuscular mycorrhizal and legume–rhizobium symbioses

et al. 2021

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Most land plants live in close contact with beneficial soil microbes: the majority of land plant species establish symbiosis with arbuscular mycorrhizal fungi, while most legumes, the third largest plant family, can form a symbiosis with nitrogen-fixing rhizobia. These microbes contribute to plant nutrition via endosymbiotic processes that require modulating the expression and function of plant transporter systems. The efficient contribution of these symbionts involves precisely controlled integration of transport, which is enabled by the adaptability and plasticity of their transporters. Advances in our understanding of these systems, driven by functional genomics research, are rapidly filling the gap in knowledge about plant membrane transport involved in these plant-microbe interactions. In this review, we synthesize recent findings associated with different stages of these symbioses, from the pre-symbiotic stage to nutrient exchange, and describe the role of host transport systems in both mycorrhizal and legume-rhizobia symbioses.

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ABCG transporters export cutin precursors for the formation of the plant cuticle

Cited by 38 publications

References 72 publications

Changes of Morphology, Chemical Compositions, and the Biosynthesis Regulations of Cuticle in Response to Chilling Injury of Banana Fruit During Storage

Changes of Morphology, Chemical Compositions, and the Biosynthesis Regulations of Cuticle in Response to Chilling Injury of Banana Fruit During Storage

Trafficking Processes and Secretion Pathways Underlying the Formation of Plant Cuticles

A roadmap of plant membrane transporters in arbuscular mycorrhizal and legume–rhizobium symbioses

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