Aberrant Expression of Long Non-coding RNAs in Exosomes in Follicle Fluid From PCOS Patients

Wang, Liping; Fan, Hairui; Zou, Yinggang; Yuan, Qiuyue; Hu, Xuming; Chen, Xujing; C, Zhu; Zhang, Xiaomei; Cui, Hengmi

doi:10.3389/fgene.2020.608178

Cited by 28 publications

(30 citation statements)

References 24 publications

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“…In this research, we found that the expression level of LRH-1 in ovarian granulosa cells in PCOS patients had a significant increase compared with the control group. After the correlation analysis between the expression of LRH-1 with the corresponding clinical data, it was found that there was a positively correlation between the expression level of LRH-1 and the AFC, AMH, LH, and T. As we all known, AFC and AMH are often used as clinical observation indicators that can reflect the ovarian reserve.And The LH and T levels were significantly higher in PCOS patients compared with non-PCOS patients [20].…”

Section: Discussionmentioning

confidence: 81%

LRH-1 high expression in the ovarian granulosa cells of PCOS patients

Yang

Wang

et al. 2021

Endocrine

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Purpose ： Polycystic ovary syndrome (PCOS) is considered as one of the most common endocrine disorder with heterogeneity. There are also reports that liver receptor homolog 1 [LRH-1 or nuclear receptor subfamily 5 group A member 2 (NR5A2)] plays an important role in the reproductive system. But up to now, there are no reports related to the link with PCOS and LRH-1. In this study, we aimed to detect the LRH-1 expression in the ovarian granulosa cell of PCOS patients and explore the potential relationship between LRH-1 and PCOS. Methods：146 follicular fluid sample were collected in this study, including 72 from PCOS patients and 74 from control patients who underwent intracytoplasmic sperm injection (ICSI) or in vitro fertilization-embryo transfer (IVF-ET). The ovarian granulosa cells were extracted from the patient's follicular fluid by magnetic-activated cell sorting (MACS) method, and the real-time quantitative PCR (qRT-PCR) was used to measure the expression of LRH-1 in ovarian granulosa cells. Then we analyzed the correlation between the expression level of LRH-1 and the clinical characteristics of patient by using Pearson Correlation analysis. Results：The expression of LRH-1 was significantly higher in PCOS patients ovarian granulosa cells than that in the control patients [vs(1.38±0.47)vs(1.03±0.32), t=5.327, p<0.0001], and it was positively correlated with antral follicles counting (AFC) (r=0.3607, p<0.0001)and the serum AMH(r=0.2662, p=0.0012)\LH(r=0.2518, p=0.0022)\T(r=0.2516, p=0.0022) in all patients. No statistical significance between LRH-1 and BMI, FSH, HOMA-IR, DHE-S, progesterone.Conclusions ： Compared with the control group, we found that LRH-1 was highly expressed in the ovarian granulosa cells of PCOS patients. Our study has revealed the relationship between the LRH-1 expression and PCOS, which suggested that LRH-1 may play an important role in ovulation disorders.While this finding provided new ideas for the study of the pathogenesis, it also provided a theoretical basis for the clinical diagnosis and treatment for PCOS.

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Section: Discussionmentioning

confidence: 81%

LRH-1 high expression in the ovarian granulosa cells of PCOS patients

Yang

Wang

et al. 2021

Endocrine

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“…were reduced in PCOS [19]. MZF1-AS1 has been reported to inhibit proline synthesis and neuroblastoma progression [31].…”

Section: Discussionmentioning

confidence: 96%

“…3A). The differentially expressed lncRNAs in this study were strictly screened according to the previous research results of Liping Wang et al [19]. There were 29 differentially expressed miRNAs constructed with 2439 differentially expressed lncRNAs in the coexpression network via the data from starBase (Table 3).…”

Section: Construction Of the Mirna-lncrna Coexpression Networkmentioning

confidence: 99%

Role of Exosomal microRNAs and lncRNAs in the Follicular Fluid of Women With Polycystic Ovary Syndrome

Ye¹,

Lin²,

Ding³

et al. 2021

Preprint

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Polycystic ovary syndrome (PCOS) is a complex class of endocrine disorders with insulin resistance, compensatory hyperinsulinaemia and obesity. However, the pathogenesis and therapies of PCOS have not been fully elucidated. Exosomal miRNAs have the potential to serve as biomarkers and therapies for a wide range of medical conditions. In this study, we isolated exosomes from follicular fluid collected from 5 PCOS patients and 5 non-PCOS patients. miRNA cDNA library sequencing identified 124 miRNAs that were significantly upregulated nearly twofold, while 33 miRNAs were significantly downregulated nearly twofold in PCOS follicular fluid exosomes. These miRNA target genes were mainly involved in metabolic pathways, pathways in cancer, the PI3K-Akt signalling pathway, the MAPK signalling pathway, endocytosis, the Ras signalling pathway, the Hippo signalling pathway, and cellular senescence. According to the previously reported exosomal lncRNA data of PCOS follicular fluid, a miRNA and lncRNA coexpression network developed from data from starBase strictly screened 29 differentially expressed miRNAs. This network also helped to identify miRNA signatures associated with metabolic processes in PCOS. Collectively, these results demonstrate the potential pathogenesis of PCOS, and follicular fluid exosomal miRNAs may be efficient targets for the diagnosis and treatment of PCOS in long-term clinical studies.

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“…A high-throughput lncRNAs sequencing study found that there are 1253 upregulated and 613 down-regulated lncRNAs in FF exosomes of patients with PCOS infertility compared with patients with non-PCOS infertility, and nine lncRNAs with significant changes may play an important role in the pathogenesis of PCOS (lncRNA-LINC00173, lncRNA-H19, lncRNA-HDAC6, lncRNA-POP4, lncRNA-PTEN, lncRNAAKT3, lncRNA-DICER1, lncRNA-NF1, and lncRNA-MUM1). Highthroughput sequencing of circRNAs also found the expression of 167 circRNAs in FF exosomes of PCOS patients are significantly upregulated and 245 circRNAs are significantly down-regulated compared with the control group, suggesting that these abnormally expressed circRNAs may play some roles in the study of pathophysiological mechanism of PCOS [76][77][78]. Similarly, proteins in exosomes of FF also changed.…”

Section: Polycystic Ovarian Syndromementioning

confidence: 89%

Roles of Extracellular Vesicles in Human Reproduction

Zhou¹,

Zhang²,

Lian³

et al. 2022

Extracellular Vesicles - Role in Diseases, Pathogenesis and Therapy

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Extracellular vesicles (EVs) are newly identified as cell-to-cell communication mediators that carry and transfer various regulatory molecules. Recent studies have shown that EVs play important roles in normal physiology and pathological conditions of human reproduction. In the female reproductive system, EVs in follicular fluid, oviduct fluid, and uterine luminal fluid are considered as vehicles to regulate follicular development, oocyte maturation and mediate embryo–maternal crosstalk to affect embryo implantation and pregnancy. In the male reproductive system, prostasomes and epididymosomes are involved in regulating sperm maturation, motility, capacitation, acrosome reaction, and fertilization. EVs transmitted cargos also play important roles in reproduction-related pathologies, such as polycystic ovarian syndrome, endometriosis, pregnancy complications, male infertility, and gynecological malignant tumors. In view of the important roles in the reproductive system, EVs may be used as biomarkers or therapeutic targets for reproductive abnormalities and related diseases. In this chapter, we sorted EVs in human reproduction through their physical/pathological functions and mechanisms, and listed several EVs as biomarkers and clinical therapeutic applications in the future.

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Aberrant Expression of Long Non-coding RNAs in Exosomes in Follicle Fluid From PCOS Patients

Cited by 28 publications

References 24 publications

LRH-1 high expression in the ovarian granulosa cells of PCOS patients

LRH-1 high expression in the ovarian granulosa cells of PCOS patients

Role of Exosomal microRNAs and lncRNAs in the Follicular Fluid of Women With Polycystic Ovary Syndrome

Roles of Extracellular Vesicles in Human Reproduction

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